Behaviour of MG-63 osteoblast-like cells on wood-based biomorphic SiC ceramics coated with bioactive glass

  • A. de Carlos
  • J. P. Borrajo
  • J. Serra
  • P. González
  • B. León
Article

Abstract

The aim of this study was to test the in vitro cytotoxicity of wood-based biomorphic Silicon Carbide (SiC) ceramics coated with bioactive glass, using MG-63 human osteoblast-like cells, with a view to their application in bone implantology. To better understand the scope of this study, it should be taken into account that biomorphic SiC ceramics have only recently been developed and this innovative product has important properties such as interconnected porosity, high strength and toughness, and easy shaping.

In the solvent extraction test, all the extracts had almost no effect on cellular activity even at 100% concentration, and cells incubated in the bioactive glass-coated SiC ceramics extracts showed a proliferation rate similar to that of the Thermanox control. There were no significant differences when the cellular attachment response of the cells on the wood-based biomorphic SiC ceramics, uncoated or coated with bioactive glass, was compared to the one exhibited by reference materials like Ti6Al4V and bulk bioactive glass. This fact looks very promising for biomedical applications.

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References

  1. 1.
    A. E. PORTER, P. TAAK, L. W. HOBBS, M. J. COATHUP, G. W. BLOOM and M. SPECTOR, Biomaterials 25 (2004) 5199.CrossRefGoogle Scholar
  2. 2.
    M. S. CUNE, I. P. VAN ROSSEN, C. DE PUTTER and R. P. J. WILS, Clin. Oral Implants Res. 7 (1996) 345.CrossRefGoogle Scholar
  3. 3.
    P. GRANT, B. ROGAARD and L. NORDSLETTEN, J. Arthroplasty 19 (2004) 274.CrossRefGoogle Scholar
  4. 4.
    G. Y. LEE, A. SRIVASTAVA, D. D. D’LIMA, P. PULIDO and C. W. COLWELL JR, Key Eng Mater 284–286 (2005) 1069.CrossRefGoogle Scholar
  5. 5.
    S. L. WHEELER, Int. J. Oral Maxillofac Implants 11 (1996) 340.Google Scholar
  6. 6.
    P. GONZÁLEZ, J. SERRA, S. LISTE, S. CHIUSSI, B. LEÓN, M. PÉREZ-AMOR, J. MARTÍNEZ-FERNÁNDEZ, A. R. DE RELLANO-LÓPEZ and F. M. VARELA-FERIA, Biomaterials 24 (2003) 4827.CrossRefGoogle Scholar
  7. 7.
    W. CAO and L. L. HENCH, Ceram Int. 22 (1996) 493.CrossRefGoogle Scholar
  8. 8.
    S. FUJIBAYASHI, M. NEO, H-M KIM, T. KOKUBO and T. NAKAMURA, Biomaterials 24 (2003) 1349.CrossRefGoogle Scholar
  9. 9.
    L. L. HENCH and O. H. ANDERSSON, edited by L. L. HENCH and J. WILSON, (World Scientific, Singapore) (1993) p. 41.Google Scholar
  10. 10.
    C. M. COTELL, D. B. CHRISEY, K. S. GRABOWSKI, J. A. SPRAGUE and C. R. GOSSET, J. Appl. Biomater. 3 (1992) 87.CrossRefGoogle Scholar
  11. 11.
    F. J. GARCÍA-SANZ, M. B. MAYOR, J. L. ARIAS, J. POU, B. LEÓN and M. PÉREZ-AMOR, J. Mater. Sci: Mater. Med. 8 (1997) 861.CrossRefGoogle Scholar
  12. 12.
    B. MAYOR, J. ARIAS, S. CHIUSSI, F. GARCIA, J. POU, B. LEÓN FONG and M. PÉREZ-MOR, Thin Solid Films 317 (1998) 363.CrossRefGoogle Scholar
  13. 13.
    H. ZENG, W. R. LACEFIELD and S. MIROV, J. Biomed. Mater. Res. 50 (2000) 248.CrossRefGoogle Scholar
  14. 14.
    L. D’ALESSIO, R. TEGHIL, M. ZACCAGNINO, I. ZACCARDO, D. FERRO and V. MAROTTA, Appl. Surf Sci. 138–139 (1999) 527.CrossRefGoogle Scholar
  15. 15.
    L. D’ALESSIO, D. FERRO, V. MAROTTA, A. SANTAGATA, R. TEGHIL and M. ZACCAGNINO, Appl. Surf Sci. 183 (2001) 10.CrossRefGoogle Scholar
  16. 16.
    J. SERRA, P. GONZALEZ, S. CHIUSSI, B. LEÓN and M. PÉREZ-AMOR, Key Eng. Mater. 192–195 (2001) 635.Google Scholar
  17. 17.
    P. GONZÁLEZ, J. SERRA, S. LISTE, S. HHIUSSI, B. LEÓN and M. PÉREZ-AMOR, Vacuum 67 (2002) 647.CrossRefGoogle Scholar
  18. 18.
    J. SERRA, S. LISTE, P. GONZÁLEZ, C. SERRA, J. P. BORRAJO, S. CHIUSSI, B. LEÓN and M. PÉREZ-AMOR, Appl. Phys. A-Mater. 79 (2004) 983.Google Scholar
  19. 19.
    S. LISTE, J. SERRA, P. GONZÁLEZ, J. P. BORRAJO, S. CHIUSSI, B. LEÓN and M. PÉREZ-AMOR, Thin Solid Films 453–454 (2004) 224.CrossRefGoogle Scholar
  20. 20.
    S. LISTE, P. GONZÁLEZ, J. SERRA, J. P. BORRAJO, S. CHIUSSI, B. LEÓN, M. PÉREZ-AMOR, J. G. LÓPEZ, F. J. FERRER, Y. MORILLA and M. A. RESPALDIZA, Thin Solid Films 453–454 (2004) 219.CrossRefGoogle Scholar
  21. 21.
    H. M. KIM, T. MIYAZAKI, T. KOKUBO and T. NAKAMURA, Key Eng. Mater. 192–195 (2001) 47.Google Scholar
  22. 22.
    T. KOKUBO, H. M. KIM and M. KAWASHITA, Biomaterials 24 (2003) 2161.CrossRefGoogle Scholar
  23. 23.
    F. LUSQUIÑOS, A. DE CARLOS, J. POU, J. L. ARIAS, M. BOUTINGUIZA, B. LEÓN, M. PÉREZ-AMOR, F. C. M. DRIESSENS, K. HING, I. GIBSON, S. BEST and W. BONFIELD, J. Biomed. Mater. Res. 64A (2003) 630.CrossRefGoogle Scholar
  24. 24.
    J. SERRA, P. GONZÁLEZ, S. LISTE, S. CHIUSSI, B. LEРN, M. PÉREZ-AMOR, H. O. YLÄNEN and M. HUPA, J. Mater. Sci.: Mater. Med. 13 (2002) 1221.CrossRefGoogle Scholar
  25. 25.
    J. P. BORRAJO, S. LISTE, J. SERRA, P. GONZÁLEZ, S. CHIUSSI, B. LEÓN, M. PÉREZ-AMOR, H. O. YLÄNEN and M. HUPA, Key Eng. Mater. 254–256 (2004) 23.CrossRefGoogle Scholar
  26. 26.
    M. AMARAL, M. A. COSTA, M. A. LOPES, R. F. SILVA, J. D. SANTOS and M. H. FERNANDES, Biomaterials 23 (2002) 4897.CrossRefGoogle Scholar
  27. 27.
    T. V. KUMARI, U. VASUDEV, A. KUMAR and B. MENON, Trends Biomater Artif Organs 15 (2002) 37.Google Scholar
  28. 28.
    N. PRICE, S. P. BENDALL, C. FRONDOZA, R. H. JINNAH and D. S. HUNGERFORD, J. Biomed. Mater. Res. 37 (1997) 394.CrossRefGoogle Scholar
  29. 29.
    M. P. FERRAZ, J. C. KNOWLES, I. OLSEN, F. J. MONTEIRO and J. D. SANTOS, Biomaterials 21 (2000) 813.CrossRefGoogle Scholar
  30. 30.
    W. C. A. VROUWENVELDER, C. G. GROOT and K. GROOT, Biomaterials 13 (1992) 382.CrossRefGoogle Scholar
  31. 31.
    W. C. A. VROUWENVELDER, C. G. GROOT and K. GROOT, J. Biomed. Mater. Res. 27 (1993) 465.CrossRefGoogle Scholar
  32. 32.
    T. MATSUDA and J. E. DAVIES, Biomaterials 8 (1987) 275.CrossRefGoogle Scholar
  33. 33.
    P. DUCHEYNE, A. EL-GHANNAM and I. SHAPIRO, J. Cell Biochem. 56 (1994) 162.CrossRefGoogle Scholar
  34. 34.
    A. OLIVA, A. SALERNO, B. LOCARDI, V. RICCIO, F. DELLA RAGIONE, P. IARDINO and V. ZAPPIA, Biomaterials 19 (1998) 1019.CrossRefGoogle Scholar
  35. 35.
    I. D. XYNOS, A. J. EDGAR, L. D. K. BUTTERY, L. L. HENCH and J. M. POLAK, J. Biomed. Mater. Res. 55 (2001) 151.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • A. de Carlos
    • 1
  • J. P. Borrajo
    • 2
  • J. Serra
    • 2
  • P. González
    • 2
  • B. León
    • 2
  1. 1.Dpto. Bioquímica, Genética e InmunologíaUniversity of VigoVigoSpain
  2. 2.Dpto. Física AplicadaUniversity of VigoVigoSpain

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