The method of surface PEGylation influences leukocyte adhesion and activation

  • Z. Ademovic
  • B. Holst
  • R. A. Kahn
  • I. Jørring
  • T. Brevig
  • J. Wei
  • X. Hou
  • B. Winter-Jensen
  • P. Kingshott
Article

Abstract

The influence of different surface modifications with poly(ethyleneglycol) (PEG) layers on the adsorption of fibrinogen and the adhesion and activation of macrophage-like human leukocytes was investigated. Poly(ethylene terephthalate) (PET) was modified using pulsed AC plasma polymerization with two types of starting monomers to generate: 1) a reactive acid surface using maleic anhydride (MAH) as monomer, and 2) a PEG-like surface using diethyleneglycol methyl vinyl ether (DEGVE) as monomer. The MAH surface was used as a reactive platform to graft linear chains of non-fouling mPEG via an intermediate layer of poly(ethyleneimine) (PEI) under lower critical solution temperature (LCST) conditions of the mPEG. The DEGVE monomer is used to create PEG-like layers by use of low power plasma conditions. The ability of the surfaces to resist protein adsorption was investigated quantitatively using 125I-radiolabeled human fibrinogen, and the conformation of the adsorbed protein was tested using an anti-fibrinogen monoclonal antibody in an enzyme-linked immunosorbent assay. The results showed that PEGylated surfaces adsorbed significantly less (up to 90% less) fibrinogen, and that unfolding of adsorbed fibrinogen was more pronounced on the linear mPEG layers than on the PEG-like plasma polymer surfaces. Adhesion of in-vitro differentiated macrophage-like U937 cells was reduced on both the PEG-like plasma polymer surfaces and the linear mPEG layers compared to the unmodified PET surface, but cells adhering to the PEG-like plasma polymer surfaces secreted less tumor necrosis factor-α (TNF- α) than cells adhering to the linear mPEG layers. In conclusion, the method for preparing non-fouling surfaces for long-term implanted devices influence surface-induced cellular responses of the host.

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Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • Z. Ademovic
    • 1
  • B. Holst
    • 2
  • R. A. Kahn
    • 2
  • I. Jørring
    • 2
  • T. Brevig
    • 2
  • J. Wei
    • 1
  • X. Hou
    • 3
  • B. Winter-Jensen
    • 1
  • P. Kingshott
    • 1
  1. 1.Danish Polymer CentreRisø National LaboratoryRoskildeDenmark
  2. 2.Bioneer A/SMammalian Cell BiologyHørsholmDenmark
  3. 3.Nuclear Research DepartmentRisø National LaboratoryRoskildeDenmark

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