Journal of Materials Science: Materials in Medicine

, Volume 17, Issue 2, pp 161–167

In vitro apatite forming ability of type I collagen hydrogels containing bioactive glass and silica sol-gel particles

  • David Eglin
  • Sonia Maalheem
  • Jacques Livage
  • Thibaud Coradin


Type I collagen hydrogel containing bioactive glass (CaO-P2O5-SiO2) and silica sol-gel micrometric particles were prepared and their in vitroapatite-forming ability in simulated body fluid assessed. X-ray diffraction and scanning electron microscopy analysis showed that bioactive glass particles entrapment in collagen matrix did not inhibit calcium phosphate formation and induced morphology variations on the crystalline phase precipitated on the hydrogel surface. The silica—collagen hydrogel composite precipitated calcium phosphate whereas silica particles and collagen hydrogel alone did not, indicating a possible synergetic effect between collagen and silica on the apatite-forming ability. Mechanisms of calcium phosphate precipitation and its relevance to biomaterial development are discussed.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    C. H. LEE, A. SINGLA and Y. LEE, Int. J. Pharm. 221 (2001) 1.CrossRefGoogle Scholar
  2. 2.
    C. A. SCOTCHFORD, M. G. CASCONE, S. DOWNES and P. GIUSTI, Biomaterials 19 (1998) 1.CrossRefGoogle Scholar
  3. 3.
    M. KIKUCHI, T. IKOMA, S. ITOH, H. N. MATSUMOTO, Y. KOYAMA, K. TAKAKUDA, K. SHINOMIYA and J. TANAKA, Comp. Sci. Tech. 64 (2004) 819.CrossRefGoogle Scholar
  4. 4.
    C. M. SERRE, M. PAPILLARD, P. CHAVASSIEUX and G. BOIVIN, Biomaterials 14 (1993) 97.CrossRefGoogle Scholar
  5. 5.
    M. MURATA, B. Z. HUANG, T. SHIBATA, S. IMAI, N. NAGAI and M. ARISUE, Int. J. Oral Maxillofac. Surg. 28 (1999) 232.CrossRefGoogle Scholar
  6. 6.
    G. BERGER, R. SAUER, G. STEINBORN, J. HINKEL, R. SCHUBERT, M. BIEDERMANN and E. WINKEL, Patent Ger. (East) DD 296065 A5 19911121 (1991) p. 11.Google Scholar
  7. 7.
    H. POHUNKOVA and M. ADAM, Biomaterials 16 (1995) 67.CrossRefGoogle Scholar
  8. 8.
    L. L. HENCH and H. PASCHALL, J. Biomed. Mater. Res. 8 (1974) 49.CrossRefGoogle Scholar
  9. 9.
    L. L. HENCH, Cur. Op. Sol. Stat. Mat. Sci. 2 (1997) 604.Google Scholar
  10. 10.
    M. M. PEREIRA, A. E. CLARK and L. L. HENCH, J. Am. Ceram. Soc. 78 (1995) 2463.CrossRefGoogle Scholar
  11. 11.
    M. M. GIRAUD-GUILLE, L. BESSEAU, D. HERBAGE and P. GOUNON, J. Struct. Biol. 113 (1994) 99.CrossRefGoogle Scholar
  12. 12.
    C. J. BRINKER and G. SCHERRER, “The Physics and Chemistry of Sol-Gel Processing” (Academic, Boston, 1990).Google Scholar
  13. 13.
    T. JAAKKOLA, J. RICH, T. TIRRI, M. JOKINEN, J. SEPPALA and A. YLI-URPO, Biomaterials 25 (2004) 575.CrossRefGoogle Scholar
  14. 14.
    T. KOKUBO, H. KUSHITANI, S. SAKKA, T. KITSUGI and T. YAMAMURO, J. Biomed Mater. Res. 24 (1990) 721.Google Scholar
  15. 15.
    JCPDS-International Centre for Diffraction Data, and American Society for Testing and Materials. Powder diffraction file. Swarthmore, PA, 2003.Google Scholar
  16. 16.
    T. CORADIN, D. EGLIN and J. LIVAGE, Spectroscopy 18 (2004) 567.Google Scholar
  17. 17.
    P. LI, C. OHTSUKI, T. KOKUBO, K. NAKANISHI, N. SOGA and K. DE GROOT, J. Biomed. Mater. Res. 28 (1994) 7.Google Scholar
  18. 18.
    H. B. WEN, J. MORIANDAN-OLDAK and A. G. Fincham, Biomaterials 22 (1999) 1717.Google Scholar
  19. 19.
    M. IIJIMA, Y. MORIWAKI and Y. KUBOKI, Cur. Top. Cry. Gro. Res. 2 (1995) 1.Google Scholar
  20. 20.
    E. K. GIRIJA, Y. YOKOGAWA and F. NAGATA, J. Mater. Sci.: Mater. Med. 15 (2004) 593.CrossRefGoogle Scholar
  21. 21.
    C. COMBES and C. REY, Biomaterials 23 (2002) 2817.CrossRefGoogle Scholar
  22. 22.
    K. HATA, T. KOKUBO, T. NAKAMURA and T. YAMAMURA, J. Am. Ceram. Soc. 78 (1995) 1049.CrossRefGoogle Scholar
  23. 23.
    S. HAYAKAWA, K. TSURU, C. OHTSUKI and A. OSAKA, J. Am. Ceram. Soc. 82 (1999) 2155.Google Scholar
  24. 24.
    A. E. PORTER, N. PATEL, J. N. SKEPPER, S. M. BEST and W. BONFIELD, Biomaterials 25 (2004) 3303.Google Scholar
  25. 25.
    E. M. CARLISLE, Science 167 (1970) 279.Google Scholar
  26. 26.
    W. J. LANDIS, D. D. LEE, J. T. BRENNA, S. CHANDRA and G. H. MORRISON, Calcif. Tissue Int. 38 (1986) 52.Google Scholar
  27. 27.
    D. EGLIN, T. CORADIN, M. M. GIRAUD-GUILLE, C. HELARY and J. LIVAGE, Biomed. Mater. Engin. 15 (2005) 43.Google Scholar
  28. 28.
    I. D. XYNOS, M. V. J. HUKKANENE, L. D. K. BUTTERY, L. L. HENCH and J. M. POLAK, Calcified Tissue Int. 67 (2000) 321.Google Scholar
  29. 29.
    T. CORADIN, M. M. GIRAUD-GUILLE, C. HELARY, J. LIVAGE and C. SANCHEZ, Mater. Res. Soc. Symp. Proc. 726 (2002) 79.Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • David Eglin
    • 1
  • Sonia Maalheem
    • 1
  • Jacques Livage
    • 1
  • Thibaud Coradin
    • 1
  1. 1.Laboratoire de Chimie de la Matière Condensée, CNRS-UMR 7574Université Pierre et Marie CurieParisFrance

Personalised recommendations