Phospholipids as implant coatings

  • R. Willumeit
  • A. Schuster
  • P. Iliev
  • S. Linser
  • F. Feyerabend
Article
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Abstract

Bio-interfaces such as bio-membranes are of outmost importance for a variety of live processes. Among them are cell-interactions which take place in, on or through cell membranes. Therefore we propose to cover metallic surfaces with phospholipids to facilitate cell-material interaction. Four lipids, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2- oleoyl-sn-glycero-3-[phospho-L-serine] (POPS) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol) (POPG), were applied to four metallic growth substrates with different surface structure, roughness and porosity. The interaction of the osteosarcoma cell line MG-63 was investigated in terms of cell adhesion and viability (MTT (methylthiazolyldiphenyl-tetrazolium bromide) assay). While POPS in general had a negative influence, the most suitable combination in terms of viability per adherent MG-63 is the coating of porous Ti6Al4V material with the phospholipids POPE or POPC. The analysis of viability of mouse macrophages RAW 264.7 and their tumor necrosis factor α (TNF-α) release showed that the adhesion and viability is worst on POPS while the TNF-α release was highest. To elucidate the potential of phospholipids to prevent or support bacterial growth, the bacterial number of Gram positive and Gram negative bacteria was investigated. For lipid concentrations higher than 1 mM in solution a growth stimulating effect independent of the lipid type was detected. On a lipid coated surface the number of bacteria was reduced by 81%, 74% and 51% for POPC, POPG and POPE.

Keywords

Lipid Lipid Layer Tissue Culture Plastic Culture Substrate Lipid Coating 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • R. Willumeit
    • 1
    • 2
  • A. Schuster
    • 1
  • P. Iliev
    • 1
  • S. Linser
    • 1
  • F. Feyerabend
    • 1
    • 2
  1. 1.GKSS Research CentreInstitute for Materials ResearchGeesthachtGermany
  2. 2.Department of Macromolecular Structure ResearchGKSS Research Centre, Institute for Materials ResearchGeesthachtGermany

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