Journal of Materials Science: Materials in Medicine

, Volume 18, Issue 2, pp 353–361

In vivo evaluation of an injectable Macroporous Calcium Phosphate Cement

  • Sergio del Valle
  • Natalia Miño
  • Fernando Muñoz
  • Antonio González
  • Josep A. Planell
  • Maria-Pau Ginebra


Although Calcium Phosphate Cements (CPC) are highly biocompatible and osteconductive materials, its resorption rate still remains too slow for some applications. In this work the introduction of Macroporosity in an injectable CPC is evaluated as a way to accelerate resorption and to increase bone ingrowth. A Macroporous and a standard CPC were injected just after preparation in a defect drilled in rabbit femur for their in vivo evaluation. The foaming agent used was Albumen, which gave up to a 75% porosity. Sodium Alginate was added to promote the cohesion of the foamed paste after implantation. In the case of the Macroporous Cement, bone growth and neovascularisation was observed inside the pores of the material, not only at the margins of the cement but also in some central pores. After 12 weeks of implantation, the residual material volume of the Macroporous Cement was approximately 35% of the initial value, whereas only the outer layers of non-Macroporous CPC were resorbed, being the residual material volume close to 100%. The higher resorption rate was due to the higher surface contact with body fluids which increased the dissolution rate, and to the enhancement of the cellular activity


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  1. 1.
    G. DACULSI, O. LABOUX, O. MALARD and P. WEISS, J. Mater. Sci. Mater. Med. 14 (2003) 195.CrossRefGoogle Scholar
  2. 2.
    M. BOHNER, Injury 31 (2000) D37.CrossRefGoogle Scholar
  3. 3.
    M. BOHNER, U. GBURECK and J. E. BARRALET, Biomaterials 26 (2005) 6423.CrossRefGoogle Scholar
  4. 4.
    E. F. BURGUERA, H. H. XU and M. D. WEIR, J. Biomed. Mater. Res. B Appl. Biomater. 77 (2006) 126.Google Scholar
  5. 5.
    M. P. GINEBRA, A. RILLIARD, E. FERNANDEZ, C. ELVIRA, J. SAN ROMAN and J. A. PLANELL, J. Biomed. Mater. Res. 57 (2001) 113.CrossRefGoogle Scholar
  6. 6.
    I. KHAIROUN, M. G. BOLTONG, F. C. DRIESSENS and J. A. PLANELL, J. Mater. Sci. Mater. Med. 9 (1998) 425.CrossRefGoogle Scholar
  7. 7.
    G. LEWIS, J. Biomed. Mater. Res. B Appl. Biomater. 76 (2006) 456.Google Scholar
  8. 8.
    M. P. GINEBRA, T. TRAYKOVA and J. A. PLANELL, Biomaterials 27 (2006) 2171.CrossRefGoogle Scholar
  9. 9.
    J. A. JANSEN, J. E. DERUIJTER, H. G. SCHAEKEN, J. P. C. M. VANDERWAERDEN, J. A. PLANELL and F. C. M. DRIESSENS, J. Mater. Sci.-Mater. Med. 6 (1995) 653.CrossRefGoogle Scholar
  10. 10.
    D. APELT, F. THEISS, A. O. EL WARRAK, K. ZLINSZKY, R. BETTSCHART-WOLFISBERGER, M. BOHNER, S. MATTER, J. A. AUER and B. VON RECHENBERG, Biomaterials 25 (2004) 1439.CrossRefGoogle Scholar
  11. 11.
    E. P. FRANKENBURG, S. A. GOLDSTEIN, T. W. BAUER, S. A. HARRIS and R. D. POSER, J. Bone Joint Surg. Am. 80 (1998) 1112.Google Scholar
  12. 12.
    S. TAKAGI and L. C. CHOW, J. Mater. Sci.-Mater. Med. 12 (2001) 135.CrossRefGoogle Scholar
  13. 13.
    Y. J. YIN, F. YE, S. CAI, K. D. YAO, J. F. CUI and X. F. SONG, J. Mater. Sci.-Mater. Med. 14 (2003) 255.CrossRefGoogle Scholar
  14. 14.
    H. H. XU and C. G. SIMON, JR., J. Biomed. Mater. Res. A 69 (2004) 267.Google Scholar
  15. 15.
    B. R. CONSTANTZ, B. M. BARR, I. C. ISON, M. T. FULMER, J. BAKER, L. MCKINNEY, S. B. GOODMAN, S. GUNASEKAREN, D. C. DELANEY, J. ROSS and R. D. POSER, J. Biomed. Mater. Res. 43 (1998) 451.CrossRefGoogle Scholar
  16. 16.
    A. ALMIRALL, J. A. DELGADO, M. P. GINEBRA and J. A. PLANELL, Bioceramics 16 (254-2) (2004) 253.Google Scholar
  17. 17.
    A. ALMIRALL, G. LARRECQ, J. A. DELGADO, S. MARTINEZ, J. A. PLANELL and M. P. GINEBRA, Biomaterials 25 (2004) 3671.CrossRefGoogle Scholar
  18. 18.
    M. P. GINEBRA, J. A. DELGADO, A. ALMIRALL, I. HARR, S. DEL VALLE and J. A. PLANELL, J. Biomed. Mater. Res. Part A, Accepted (2006).Google Scholar
  19. 19.
    M. P. GINEBRA, E. FERNANDEZ, E. A. DE MAEYER, R. M. VERBEECK, M. G. BOLTONG, J. GINEBRA, F. C. DRIESSENS and J. A. PLANELL, J. Dent. Res. 76 (1997) 905.CrossRefGoogle Scholar
  20. 20.
    E. FERNANDEZ, M. G. BOLTONG, M. P. GINEBRA, F. C. M. DRIESSENS, O. BERMUDEZ and J. A. PLANELL, J. Mater. Sci. Lett. 15 (1996) 1004.CrossRefGoogle Scholar
  21. 21.
    D. W. JACKSON, P. A. LALOR, H. M. ABERMAN and T. M. SIMON, J. Bone Joint Surg. Am. 83-A (2001) 53.Google Scholar
  22. 22.
    A. M. PARFITT, M. K. DREZNER, F. H. GLORIEUX, J. A. KANIS, H. MALLUCHE, P. J. MEUNIER, S. M. OTT and R. R. RECKER, J. Bone Miner. Res. 2 (1987) 595.CrossRefGoogle Scholar
  23. 23.
    R. P. DEL REAL, E. OOMS, J. G. WOLKE, M. VALLET-REGI and J. A. JANSEN, J. Biomed. Mater. Res. A 65 (2003) 30.Google Scholar
  24. 24.
    R. O. HUSE, P. QUINTEN RUHE, J. G. C. WOLKE and J. A. JANSEN, Clini. Oral Impl. Res. 15 (2004) 741.CrossRefGoogle Scholar
  25. 25.
    Y. MIYAMOTO, K. ISHIKAWA, H. FUKAO, M. SAWADA, M. NAGAYAMA, M. KON and K. ASAOKA, Biomaterials 16 (1995) 855.CrossRefGoogle Scholar
  26. 26.
    Y. MIYAMOTO, K. ISHIKAWA, M. TAKECHI, T. TOH, T. YUASA, M. NAGAYAMA and K. SUZUKI, J. Biomed. Mater. Res. 48 (1999) 36.CrossRefGoogle Scholar
  27. 27.
    K. KURASHINA, H. KURITA, M. HIRANO, A. KOTANI, C. P. KLEIN and K. DE GROOT, Biomaterials 18 (1997) 539.CrossRefGoogle Scholar
  28. 28.
    B. FLAUTRE, C. DELECOURT, M. C. BLARY, P. VAN LANDUYT, J. LEMAITRE and P. HARDOUIN, Bone 25 (1999) 35S.CrossRefGoogle Scholar
  29. 29.
    O. GAUTHIER, I. KHAIROUN, J. BOSCO, L. OBADIA, X. BOURGES, C. RAU, D. MAGNE, J. M. BOULER, E. AGUADO, G. DACULSI and P. WEISS, J. Biomed. Mater. Res. A 66 (2003) 47.Google Scholar
  30. 30.
    O. GAUTHIER, E. GOYENVALLE, J. M. BOULER, J. GUICHEUX, P. PILET, P. WEISS and G. DACULSI, J. Mater. Sci. Mater. Med. 12 (2001) 385.CrossRefGoogle Scholar
  31. 31.
    S. F. HULBERT, KLAWITTE.JJ, F. A. YOUNG, R. S. MATHEWS, C. D. TALBERT, J. C. MORRISON, J. C. CHAPIN, F. M. KING, T. L. BRIDGES and C. N. WILSON, Amer. Cer. Soci. Bull.. 49 (1970) 482.Google Scholar
  32. 32.
    R. HOLMES and H. HAGLER, J. Cranio-Maxillofacial Surgery 16 (1988) 199.CrossRefGoogle Scholar
  33. 33.
    B. FLAUTRE, M. DESCAMPS, C. DELECOURT, M. C. BLARY and P. HARDOUIN, J. Mater. Sci. Mater. Med. 12 (2001) 679.CrossRefGoogle Scholar
  34. 34.
    H. YUAN, K. KURASHINA, J. D. DE BRUIJN, Y. LI, K. DE GROOT and X. ZHANG, Biomaterials 20 (1999) 1799.CrossRefGoogle Scholar
  35. 35.
    R. Z. LEGEROS, S. LIN, R. ROHANIZADEH, D. MIJARES and J. P. LEGEROS, J. Mater. Sci. Mater. Med. 14 (2003) 201.CrossRefGoogle Scholar
  36. 36.
    B. BOURGEOIS, O. LABOUX, L. OBADIA, O. GAUTHIER, E. BETTI, E. AGUADO, G. DACULSI and J. M. BOULER, J. Biomed. Mater. Res. A 65 (2003) 402.Google Scholar
  37. 37.
    J. X. LU, A. GALLUR, B. FLAUTRE, K. ANSELME, M. DESCAMPS, B. THIERRY and P. HARDOUIN, J. Biomed. Mater. Res. 42 (1998) 357.CrossRefGoogle Scholar
  38. 38.
    C. T. WONG, W. W. LU, W. K. CHAN, K. M. CHEUNG, K. D. LUK, D. S. LU, A. B. RABIE, L. F. DENG and J. C. LEONG, J. Biomed. Mater. Res. A 68 (2004) 513.Google Scholar

Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Sergio del Valle
    • 1
  • Natalia Miño
    • 2
  • Fernando Muñoz
    • 2
  • Antonio González
    • 2
  • Josep A. Planell
    • 1
  • Maria-Pau Ginebra
    • 1
  1. 1.Biomaterials Division, Department of Materials Science and Metallurgy, Biomedical Engineering Research CentreTechnical University of Catalonia (UPC)BarcelonaSpain
  2. 2.Unidad de Cirugía, Dpto. Ciencias Clínicas VeterinariasUniversidad de Santiago de Compostela, Campus universitarioLugoSpain

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