In vitro and in vivo biocompatibility of graded hydroxyapatite–zirconia composite bioceramic

  • Renfu Quan
  • Disheng Yang
  • Xiaochun Wu
  • Hongbin Wang
  • Xudong Miao
  • Wei Li


To obtain bioceramics with good osteoinductive ability and mechanical strength, graded hydroxyapatite–zirconia (HA–ZrO2) composite bioceramics were prepared in this work. The biocompatibility of the bioceramics was investigated in vitro based on acute toxicity and cytotoxicity tests and hemolysis assay. Results showed the studied graded HA–ZrO2 had little toxicity to mouse and L929 mouse fibroblasts. Also, hemolysis assay indicated a good blood compatibility of the bioceramics. Based on the results of in vitro tests, animal experiments were performed on white New Zealand rabbits by implantation into hip muscles and femur. It was found that the graded HA–ZrO2 composite bioceramics exhibited superior osteoinductive ability, which may be a promising bioceramics implant.



The financial support from the Ministry of Science and Technology of China is gratefully acknowledged.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Renfu Quan
    • 1
    • 2
  • Disheng Yang
    • 1
  • Xiaochun Wu
    • 3
  • Hongbin Wang
    • 3
  • Xudong Miao
    • 3
  • Wei Li
    • 3
  1. 1.Department of OrthopaedicsSecond Affiliated Hospital, Zhejiang UniversityHangzhouChina
  2. 2.Department of OrthopaedicsXiaoshan Traditional Chinese Medical Hospital, Zhejiang UniversityHangzhouChina
  3. 3.School of Materials Science and EngineeringShanghai UniversityShanghaiChina

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