In vitro and in vivo biocompatibility of graded hydroxyapatite–zirconia composite bioceramic

  • Renfu Quan
  • Disheng Yang
  • Xiaochun Wu
  • Hongbin Wang
  • Xudong Miao
  • Wei Li
Article

Abstract

To obtain bioceramics with good osteoinductive ability and mechanical strength, graded hydroxyapatite–zirconia (HA–ZrO2) composite bioceramics were prepared in this work. The biocompatibility of the bioceramics was investigated in vitro based on acute toxicity and cytotoxicity tests and hemolysis assay. Results showed the studied graded HA–ZrO2 had little toxicity to mouse and L929 mouse fibroblasts. Also, hemolysis assay indicated a good blood compatibility of the bioceramics. Based on the results of in vitro tests, animal experiments were performed on white New Zealand rabbits by implantation into hip muscles and femur. It was found that the graded HA–ZrO2 composite bioceramics exhibited superior osteoinductive ability, which may be a promising bioceramics implant.

Notes

Acknowledgements

The financial support from the Ministry of Science and Technology of China is gratefully acknowledged.

References

  1. 1.
    P. R. A. KUMAR, H. K. VARMA and T. V. KUMARY, Acta Biomater. 1 (2005) 545CrossRefGoogle Scholar
  2. 2.
    R. KUMAR, P. CHEANG and K. A. KHOR, Biomaterials 24 (2003) 2611CrossRefGoogle Scholar
  3. 3.
    H. W. KIM, Y. M. KONG, C. J. BAE, Y. J. NOH and H. E. KIM, Biomaterials 25 (2004) 2919CrossRefGoogle Scholar
  4. 4.
    X. G. MIAO, Y. M. CHEN, H. B. GUO and K. A. KHOR, Ceram. Int. 30 (2004) 1793CrossRefGoogle Scholar
  5. 5.
    V. V. SILVA, F. S. LAMEIRAS and R. Z. DOMINGUES, Compos. Sci. Technol. 61 (2001) 301CrossRefGoogle Scholar
  6. 6.
    K. YOSHIDA, K. HASHIMOTO, Y. TODA, S. UDAGAWA and T. KANAZAWA, J. Eur. Ceram. Soc. 26 (2006) 515CrossRefGoogle Scholar
  7. 7.
    R. KUMAR, P. CHEANG and K. A. KHOR, J. Mater. Proc. Technol. 140 (2003) 420CrossRefGoogle Scholar
  8. 8.
    R. R. RAO and T. S. KANNAN, Mater. Sci. Eng. C 20 (2002) 187CrossRefGoogle Scholar
  9. 9.
    M. SIVAKUMAR and I. MANJUBALA, Mater. Lett. 50 (2001) 199CrossRefGoogle Scholar
  10. 10.
    B. Y. CHOU and E. CHANG, Scripta Mater. 45 (2001) 487CrossRefGoogle Scholar
  11. 11.
    S. PADILLA, J. ROMAN, S. SANCHEZ-SALCEDO and M. VALLET-REGI, Acta Biomater. 2 (2006) 331CrossRefGoogle Scholar
  12. 12.
    H. W. KIM and H. E. KIM, J. Biomed. Mater. Res. Part B 77B (2006) 323CrossRefGoogle Scholar
  13. 13.
    Y. M. KONG, C. J. BAE, S. H. LEE, H. W. KIM and H. E. KIM, Biomaterials 26 (2005) 509CrossRefGoogle Scholar
  14. 14.
    T. SUZUKI, S. FUJIBAYASHI, Y. NAKAGAWA, I. NODA and T. NAKAMURA, Biomaterials 27 (2006) 996CrossRefGoogle Scholar
  15. 15.
    N. KAWASHIMA, K. SOETANTO, K. WATANABE, K. ONO and T. MATSUNO, Colloid Surf. B 10 (1997) 23CrossRefGoogle Scholar
  16. 16.
    T. SUZUKI et al., J. Biosci. Bioeng. 89 (2000) 18CrossRefGoogle Scholar
  17. 17.
    R. B. HEIMANN, H. KURZWEG, D. G. IVEY and M. L. WAYMAN, J. Biomed. Mater. Res. 43 (1998) 441CrossRefGoogle Scholar
  18. 18.
    S. FOPPIANO, S. J. MARSHALL, E. SAIZ, A. P. TOMSIA and G. W. MARSHALL, Acta Biomater. 2 (2006) 133CrossRefGoogle Scholar
  19. 19.
    E. S. AHN, N. J. GLEASON, A. NAKAHIRA and J. Y. YING, Nano Lett. 1 (2001) 149CrossRefGoogle Scholar
  20. 20.
    M. INUZUKA, et al., Solid State Ionics 172 (2004) 509CrossRefGoogle Scholar
  21. 21.
    V. V. SILVA, F. S. LAMEIRAS and R. Z. DOMINGUES, Ceram. Int. 27 (2001) 615CrossRefGoogle Scholar
  22. 22.
    A. Y. SADL, M. A. SHOKRGOZAR, S. S. HOMAELGOHAR, M. HOSSELNALLPOUR, A. KHAVANDL and J. JAVADPOUR, J. Mater. Sci.: Mater. Med. 17 (2006) 407CrossRefGoogle Scholar
  23. 23.
    H. SIMANK, M. STUBER, R. FRAHM, L. HELBIG, H. V. LENTHE and R. MULLER, Biomaterials 27 (2006) 3988CrossRefGoogle Scholar
  24. 24.
    R. F. QUAN, D. S. YANG, X. C. WU, H. B. WANG, X. D. MIAO and W. LI, J. Biomater. Appl. in pressGoogle Scholar
  25. 25.
    B. J. NABLO and M. H. SCHOENFISCH, Biomaterials 26 (2005) 4405CrossRefGoogle Scholar
  26. 26.
    L.-S. WAN, Z.-K. XU, X.-J. HUANG, Z.-G. WANG and J.-L. WANG, Polymer 46 (2005) 7715CrossRefGoogle Scholar
  27. 27.
    L.-S. WAN, Z.-K. XU, X.-J. HUANG, Z.-G. WANG and P. YE, Macromol. Biosci. 5 (2005) 229CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Renfu Quan
    • 1
    • 2
  • Disheng Yang
    • 1
  • Xiaochun Wu
    • 3
  • Hongbin Wang
    • 3
  • Xudong Miao
    • 3
  • Wei Li
    • 3
  1. 1.Department of OrthopaedicsSecond Affiliated Hospital, Zhejiang UniversityHangzhouChina
  2. 2.Department of OrthopaedicsXiaoshan Traditional Chinese Medical Hospital, Zhejiang UniversityHangzhouChina
  3. 3.School of Materials Science and EngineeringShanghai UniversityShanghaiChina

Personalised recommendations