Journal of Materials Science: Materials in Medicine

, Volume 16, Issue 12, pp 1131–1136 | Cite as

Soluble phosphate glass fibres for repair of bone-ligament interface

  • M. Bitar
  • J. C. Knowles
  • M. P. Lewis
  • V. SalihEmail author


Phosphate-based fibres of the generic composition (CaO)0.46–(Na2O) n –(Fe2O3) y –(P2O5)0.50 have been evaluated, in vitro, as three dimensional scaffolds for tissue engineering of the hard-soft tissue interface by assessing the fibre solubility and growth and functional gene expression of human cells. Primary human osteoblasts and fibroblasts were seeded onto scaffolds and maintained in culture for up to 21 days. Fluorescent immunolabeling revealed the spread cell morphology and significant proliferation pattern on these fibres, particularly on the 3 mol% Fe2O3-containing formulation. Real-time quantitative Polymerase Chain Reaction (rtQ-PCR) analysis of gene expression using TaqMan® Probes was preformed and it has been established that committed cell differentiation was maintained by both cell types, and was strongly related to the 3 mol% Fe2O3 glass composition. These novel, readily manufactured, soluble glass fibres offer a biocompatible and biochemically favourable alternative in the search for suitable degradable materials used in Tissue Engineering.


Fe2O3 Na2O Quantitative Polymerase Chain Reaction Phosphate Glass Soluble Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • M. Bitar
    • 1
  • J. C. Knowles
    • 1
  • M. P. Lewis
    • 1
  • V. Salih
    • 1
    Email author
  1. 1.Department of Biomaterials/Tissue Engineering, Eastman Dental InstituteUniversity College London (UCL)LondonUK

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