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Journal of Materials Science: Materials in Medicine

, Volume 16, Issue 12, pp 1121–1124 | Cite as

Hydroxyapatite scaffolds for bone tissue engineering made by 3D printing

  • Barbara Leukers
  • Hülya Gülkan
  • Stephan H. Irsen
  • Stefan Milz
  • Carsten Tille
  • Matthias Schieker
  • Hermann Seitz
Article

Abstract

Nowadays, there is a significant need for synthetic bone replacement materials used in bone tissue engineering (BTE). Rapid prototyping and especially 3D printing is a suitable technique to create custom implants based on medical data sets. 3D printing allows to fabricate scaffolds based on Hydroxyapatite with complex internal structures and high resolution. To determine the in vitro behaviour of cells cultivated on the scaffolds, we designed a special test-part. MC3T3-E1 cells were seeded on the scaffolds and cultivated under static and dynamic setups. Histological evaluation was carried out to characterise the cell ingrowth. In summary, the dynamic cultivation method lead to a stronger population compared to the static cultivation method. The cells proliferated deep into the structure forming close contact to Hydroxyapatite granules.

Keywords

Hydroxyapatite Rapid Prototype Bone Tissue Engineering Replacement Material Dynamic Cultivation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Barbara Leukers
    • 1
  • Hülya Gülkan
    • 2
  • Stephan H. Irsen
    • 1
  • Stefan Milz
    • 3
  • Carsten Tille
    • 1
  • Matthias Schieker
    • 2
  • Hermann Seitz
    • 1
  1. 1.Research Center CaesarBonn
  2. 2.Experimental Surgery and Regenerative Medicine, Department of Surgery—DowntownUniversity of MunichMünchen
  3. 3.AO Research InstituteDavosSwitzerland

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