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Journal of Materials Science: Materials in Medicine

, Volume 16, Issue 9, pp 807–819 | Cite as

Osteogenic differentiation of mesenchymal progenitor cells in computer designed fibrin-polymer-ceramic scaffolds manufactured by fused deposition modeling

  • Jan-Thorsten SchantzEmail author
  • Arthur Brandwood
  • Dietmar Werner Hutmacher
  • Hwei Ling Khor
  • Katharina Bittner
Article

Abstract

Biomimetic scaffolds offer great potentials in the development of bone analogs for tissue engineering. The studies presented in this paper focus specifically on the osteogenic potential of the novel PCL/CaP matrices and its degradation behavior. Biodegradable Polymer-ceramic Scaffolds were fabricated using the solid free form fabrication technology: Fused Deposition Modeling (FDM). The scaffold architecture was characterized by a honeycomb-like design and a complete interconnectivity of the pores. Human mesenchymal stem cells (MSCs) were seeded together with fibrin glue into PCL/CaP scaffolds and cultured in vitro for periods of up to eight weeks. Cellular adhesion, proliferation and osteogenic differentiation were assessed in these constructs using a range of histological and microscopic techniques. In additional experiments, degradation was assessed by measuring mass loss, diameter change, molecular weight change and by scanning electron micrographs. MSCs were able to adhere, migrate, and differentiate along the osteogenic lineage with in these scaffolds. The PCL/CaP scaffolds showed up to 27 fold increased degradation of compared to PCL scaffolds.

Keywords

Mesenchymal Stem Cell Osteogenic Differentiation Scan Electron Micrographs Fibrin Glue Human Mesenchymal Stem Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Jan-Thorsten Schantz
    • 1
    • 2
    Email author
  • Arthur Brandwood
    • 5
  • Dietmar Werner Hutmacher
    • 1
    • 3
  • Hwei Ling Khor
    • 1
    • 4
  • Katharina Bittner
    • 6
  1. 1.Division of BioengineeringNational University of SingaporeSingapore
  2. 2.Division of Plastic SurgeryNational University of SingaporeSingapore
  3. 3.Department of Orthopedic SurgeryNational University of SingaporeSingapore
  4. 4.Department of Material ScienceNational University of SingaporeSingapore
  5. 5.Graduate School of Biomedical EngineeringUniversity of New South WalesSydneyAustralia
  6. 6.Baxter BiosurgeryMunichGermany

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