Macroporous hydrogels based on 2-hydroxyethyl methacrylate
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Four series of macroporous hydrogels based on crosslinked copolymers of 2-hydroxyethyl methacrylate (HEMA)—sodium methacrylate (MANa), copolymer HEMA—[2-(methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl), terpolymer HEMA—MANa—MOETACl and on a polyelectrolyte complex were used as carriers for immobilization of proteins, chicken egg white albumin and avidin. The adsorption capacity of the hydrogels for the two proteins, kinetics and pH dependence of albumin adsorption and desorption were studied. The morphology of the hydrogels with and without immobilized albumin was studied by low-vacuum scanning electron microscopy.
KeywordsScanning Electron Microscopy Albumin Immobilization Adsorption Capacity Methacrylate
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