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Macroporous hydrogels based on 2-hydroxyethyl methacrylate

Part III Hydrogels as carriers for immobilization of proteins
  • J. MicháalekEmail author
  • M. Přáadný
  • A. Artyukhov
  • M. šlouf
  • K. SmetanaJr.
Article

Abstract

Four series of macroporous hydrogels based on crosslinked copolymers of 2-hydroxyethyl methacrylate (HEMA)—sodium methacrylate (MANa), copolymer HEMA—[2-(methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl), terpolymer HEMA—MANa—MOETACl and on a polyelectrolyte complex were used as carriers for immobilization of proteins, chicken egg white albumin and avidin. The adsorption capacity of the hydrogels for the two proteins, kinetics and pH dependence of albumin adsorption and desorption were studied. The morphology of the hydrogels with and without immobilized albumin was studied by low-vacuum scanning electron microscopy.

Keywords

Scanning Electron Microscopy Albumin Immobilization Adsorption Capacity Methacrylate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • J. Micháalek
    • 1
    • 3
    Email author
  • M. Přáadný
    • 1
    • 3
  • A. Artyukhov
    • 1
    • 2
  • M. šlouf
    • 1
  • K. SmetanaJr.
    • 3
    • 4
  1. 1.Institute of Macromolecular ChemistryAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  2. 2.D. I. Mendeleev University of Chemical Technology of RussiaMoscowRussia
  3. 3.Center for Cell Therapy and Tissue RepairCharles UniversityPrague 6Czech Republic
  4. 4.Institute of Anatomy, 1st Faculty of MedicineCharles UniversityPrague 2Czech Republic

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