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Detection of ultra-trace levels of insulin by Fe3O4@MoS2/rGO-GCE as a sensor based on isoelectric points

  • Nahid Askari
  • Amirkhosro Beheshti-Marnani
  • Mohammad Bagher AskariEmail author
  • Tahereh Rohani
Article
  • 14 Downloads

Abstract

A transition metal dichalcogenide (TMD) composite consisted of MoS2 coated magnetite nanoparticles, hybridized with reduced graphene oxide, loaded on the surface of a glassy carbon electrode (Fe3O4@MoS2/rGO-GCE) was applied for detection of trace amounts of in vitro insulin based on isoelectric points of the modifier and the insulin. The nanocomposite was characterized by X-ray diffraction (XRD), energy dispersive X-Ray analysis (EDX), field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM). Differential pulse voltammetry (DPV) was applied for quantitative analysis of insulin and a wide linear range of insulin concentration (10–1800 nM) was obtained along with good linearity (r2 = 0.992), significant limit of detection (5.4 nM) and grate reproducibility (RSD% = 2.03). Based on our knowledge, it is the first report for detection of insulin as a bio-macromolecule in partnership with a composite of transition metal dichalcogenides.

Notes

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Nahid Askari
    • 1
  • Amirkhosro Beheshti-Marnani
    • 2
  • Mohammad Bagher Askari
    • 3
    • 4
    Email author
  • Tahereh Rohani
    • 2
  1. 1.Department of Biotechnology, Institute of Sciences and High Technology and Environmental SciencesGraduate University of Advanced TechnologyKermanIran
  2. 2.Department of ChemistryPayame Noor University (PNU)TehranIran
  3. 3.Department of Physics, Faculty of SciencesUniversity of GuilanRashtIran
  4. 4.Department of PhysicsPayame Noor University (PNU)TehranIran

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