Journal of Materials Science

, Volume 55, Issue 11, pp 4830–4847 | Cite as

Antibacterial Al-doped ZnO coatings on PLA films

  • Daniele ValeriniEmail author
  • Loredana Tammaro
  • Fulvia Villani
  • Antonella Rizzo
  • Ivana Caputo
  • Gaetana Paolella
  • Giovanni Vigliotta
Materials for life sciences


Antimicrobial surfaces can play a key role in many fields, like biomedical applications and food packaging, where it is fundamental to prevent clinical infections, foodborne diseases, or quality loss of food and beverages. Materials used for such kind of applications should be biocompatible or avoid harmful effects on human health. Additionally, since a wide variety of tools and components operating in contact with human tissues or foodstuff are made of plastic-based materials, it is important to investigate antibacterial surfaces applied in conjunction with eco-friendly bio-based plastics. For all these reasons, here we propose the use of novel composites made of nanostructured aluminum-doped zinc oxide antimicrobial coatings on polylactide films. Their surface topography is inspected at sub- and supra-micron length scales, and its influence on wettability and antibacterial activity is investigated, together with FTIR analysis for identification of molecular vibrations. Their strong antimicrobial performance is assessed against four different bacterial strains, and their safety is evaluated on two different human cell lines. The results here presented identify AZO-coated PLA films as very appealing eco-friendly antibacterial systems for food packaging and biomedical applications.


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Auras R, Harte B, Selke S (2004) An overview of polylactides as packaging materials. Macromol Biosci 4:835–864. CrossRefGoogle Scholar
  2. 2.
    Tawakkal ISMA, Cran MJ, Miltz J, Bigger SW (2014) A review of poly(lactic acid)-based materials for antimicrobial packaging. J Food Sci 79:R1477–R1490. CrossRefGoogle Scholar
  3. 3.
    He X, Hwang H-M (2016) Nanotechnology in food science: Functionality, applicability, and safety assessment. J Food Drug Anal 24:671–681. CrossRefGoogle Scholar
  4. 4.
    Garcia CV, Shin GH, Kim JT (2018) Metal oxide-based nanocomposites in food packaging: applications, migration, and regulations. Trends Food Sci Technol 82:21–31. CrossRefGoogle Scholar
  5. 5.
    Gold K, Slay B, Knackstedt M, Gaharwar AK (2018) Antimicrobial activity of metal and metal-oxide based nanoparticles. Adv Ther 1:1700033. CrossRefGoogle Scholar
  6. 6.
    Raghunath A, Perumal E (2017) Metal oxide nanoparticles as antimicrobial agents: a promise for the future. Int J Antimicrob Agents 49:137–152. CrossRefGoogle Scholar
  7. 7.
    Khezerlou A, Alizadeh-Sani M, Azizi-Lalabadi M, Ehsani A (2018) Nanoparticles and their antimicrobial properties against pathogens including bacteria, fungi, parasites and viruses. Microb Pathog 123:505–526. CrossRefGoogle Scholar
  8. 8.
    Nair S, Sasidharan A, DivyaRani VV, Menon D, Nair S, Manzoor K, Raina S (2009) Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells. J Mater Sci Mater Med 20:S235–S241. CrossRefGoogle Scholar
  9. 9.
    Kumar R, Umar A, Kumar G, SinghNalwa H (2017) Antimicrobial properties of ZnO nanomaterials: a review. Ceram Int 43:3940–3961. CrossRefGoogle Scholar
  10. 10.
    Sruthi S, Ashtami J, Mohanan PV (2018) Biomedical application and hidden toxicity of Zinc oxide nanoparticles. Mater Today Chem 10:175–186. CrossRefGoogle Scholar
  11. 11.
    Valerini D, Tammaro L, Di Benedetto F, Vigliotta G, Capodieci L, Terzi R, Rizzo A (2018) Aluminum-doped zinc oxide coatings on polylactic acid films for antimicrobial food packaging. Thin Solid Films 645:187–192. CrossRefGoogle Scholar
  12. 12.
    Saxena V, Chandra P, Pandey LM (2018) Design and characterization of novel Al-doped ZnO nanoassembly as an effective nanoantibiotic. Appl Nanosci 8:1925–1941. CrossRefGoogle Scholar
  13. 13.
    Chidhambaram N (2019) Augmented antibacterial efficacies of the aluminium doped ZnO nanoparticles against four pathogenic bacteria. Mater Res Express 6:075061. CrossRefGoogle Scholar
  14. 14.
    Denes E, Barrière G, Poli E, Lévêque G (2018) Alumina biocompatibility. J Longterm Eff Med 28:9–13. CrossRefGoogle Scholar
  15. 15.
    Commission Regulation (EU) No 10/2011 of 14 January 2011 on plastic materials and articles intended to come into contact with foodGoogle Scholar
  16. 16.
    Murariu M, Doumbia A, Bonnaud L, Dechief AL, Paint Y, Ferreira M, Campagne C, Devaux E, Dubois P (2011) High-performance polylactide/ZnO nanocomposites designed for films and fibers with special end-use properties. Biomacromol 12:1762–1771. CrossRefGoogle Scholar
  17. 17.
    Pantani R, Gorrasi G, Vigliotta G, Murariu M, Dubois P (2013) PLA-ZnO nanocomposite films: water vapor barrier properties and specific end-use characteristics. Eur Polym J 49:3471–3482. CrossRefGoogle Scholar
  18. 18.
    Martucciello S, Paolella G, Muzashvili T, Skhirtladze A, Pizza C, Caputo I, Piacente S (2018) Steroids from Helleborus caucasicus reduce cancer cell viability inducing apoptosis and GRP78 down-regulation. Chem Biol Interact 279:43–50. CrossRefGoogle Scholar
  19. 19.
    Varshosaz J, Hassanzadeh F, SadeghiAliabadi H, Nayebsadrian M, Banitalebi M, Rostami M (2014) Synthesis and characterization of folate-targeted dextran/retinoic acid micelles for doxorubicin delivery in acute leukemia. Biomed Res Int 2014:525684. CrossRefGoogle Scholar
  20. 20.
    Copinet A, Bertrand C, Govindin S, Coma V, Couturier Y (2004) Effects of ultraviolet light (315 nm), temperature and relative humidity on the degradation of polylactic acid plastic films. Chemosphere 55:763–773. CrossRefGoogle Scholar
  21. 21.
    Ho K-LG, Pometto AL III (1999) Effects of electron-beam irradiation and ultraviolet light (365 nm) on polylactic acid plastic films. J Environ Polym Degrad 7:93–100. CrossRefGoogle Scholar
  22. 22.
    Saravanakumar K, Ravichandran K (2012) Synthesis of heavily doped nanocrystalline ZnO: Al powders using a simple soft chemical method. J Mater Sci: Mater Electron 23:1462–1469. CrossRefGoogle Scholar
  23. 23.
    Djelloul A, Aida MS, Bougdira J (2010) Photoluminescence, FTIR and X-ray diffraction studies on undoped and Al-doped ZnO thin films grown on polycrystalline α-alumina substrates by ultrasonic spray pyrolysis. J Lumin 130:2113–2117. CrossRefGoogle Scholar
  24. 24.
    Wan C, Tan H, Jin S, Yang H, Tang M, He J (2008) Highly conductive Al-doped tetra-needle-like ZnO whiskers prepared by a solid state method. Mater Sci Eng B 150:203–207. CrossRefGoogle Scholar
  25. 25.
    Vasconcelos DCL, Nunes EHM, Vasconcelos WL (2012) AES and FTIR characterization of sol–gel alumina films. J Non-Cryst Solids 358:1374–1379. CrossRefGoogle Scholar
  26. 26.
    Ström G, Fredriksson M, Stenius P (1987) Contact angles, work of adhesion, and interfacial tensions at a dissolving Hydrocarbon surface. J Colloid Interface Sci 119:352–361. CrossRefGoogle Scholar
  27. 27.
    Hsueh Y-H, Ke W-J, Hsieh C-T, Lin K-S, Tzou D-Y, Chiang C-L (2015) ZnO nanoparticles affect bacillus subtilis cell growth and biofilm formation. PLoS ONE 10:e0128457. CrossRefGoogle Scholar
  28. 28.
    Cao F, Zhang L, Wang H, You Y, Wang Y, Gao N, Ren J, Qu X (2019) Defect-rich adhesive nanozymes as efficient antibiotics for enhanced bacterial inhibition. Angew Chem Int Ed 58:16236–16242. CrossRefGoogle Scholar
  29. 29.
    Reddy KM, Feris K, Bell J, Wingett DG, Hanley C, Punnoose A (2007) Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems. Appl Phys Lett 90:213902. CrossRefGoogle Scholar
  30. 30.
    Siddiqi KS, Rahman AU, Husen TA (2018) Properties of zinc oxide nanoparticles and their activity against microbes. Nanoscale Res Lett 13:141. CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Laboratory for Functional Materials and Technologies for Sustainable Applications (SSPT-PROMAS-MATAS)ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic DevelopmentBrindisiItaly
  2. 2.Nanomaterials and Devices Laboratory (SSPT-PROMAS-NANO)ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic DevelopmentPortici, NaplesItaly
  3. 3.Department of Chemistry and Biology “A. Zambelli” (DCB)University of SalernoFiscianoItaly

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