Effect of the dual incorporation of fullerene and polyethyleneimine moieties into SBA-15 materials as platforms for drug delivery
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Mesostructured SBA-15 silica materials have been successfully dual functionalized with polyethyleneimine (PEI) groups and C60 fullerene moieties to allow an evaluation of their properties as nanovehicles for controlled drug delivery. Methylprednisolone sodium succinate was selected as a model drug for adsorption on the surface of functionalized SBA-15 silica materials. The resulting dual-functionalized SBA-15 silica materials exhibit mesoscopic arrangements, although with a remarkable reduction in their textural properties as compared to pure silica SBA-15. The adsorption capacity of methylprednisolone on functionalized SBA-15-PEI improved remarkably compared with that of raw SBA-15, while the drug release rate slowed, as the amount of PEI anchored in the SBA-15 increased. The strong attractive electrostatic interactions between methylprednisolone and the silica surfaces of SBA-15-PEI materials, measured by zeta potential, account for these results. In a second step, wherein C60 fullerene species in combination with PEI were grafted to the silica, the results establish that the steric effects and hydrophobicity of the C60 moieties hinder methylprednisolone transport within the silica pores. The kinetic parameters obtained from the drug release profiles, fitted to four kinetic models, show that the incorporation of C60 species yields lower methylprednisolone release rates from SBA-15-PEI-C60 materials than from SBA-15-PEI materials. Additionally, the incorporation of fullerene groups into PEI-modified materials provides an increment in cell viability. Confocal microscopy evidences the cellular internalization of the dual-functionalized mesoporous SBA-15 materials inside the plasmatic membrane.
The financial support of the Spanish government (CTQ2014-57858-R and CTQ2017-88642-R) is gratefully acknowledged. J. Ortiz-Bustos thanks the “Ministerio de Economía y Competitividad” of Spain (BES-2012-056525) for the research internship at the Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, Finland. The authors thank Dr. Victor de la Peña from the IMDEA Energy Institute, Photoactivated processes Unit, for helping in XPS discussion.
- 1.Vallet-Regí M, Colilla M, Izquierdo-Barba I, Manzano M (2018) Mesoporous silica nanoparticles for drug delivery: current insights. Molecules 23:1–19Google Scholar
- 4.Morales V, Gutiérrez-Salmerón M, Balabasquer M, Ortiz-Bustos J, Chocarro-Calvo A, García-Jiménez C, García-Muñoz RA (2016) New drug-structure-directing agent concept: inherent pharmacological activity combined with templating solid and hollow-shell mesostructured silica nanoparticles. Adv Funct Mater 26(40):7291–7303CrossRefGoogle Scholar
- 8.García-Muñoz RA, Morales V, Linares M, González PE, Sanz R, Serrano DP (2014) Influence of the structural and textural properties of ordered mesoporous materials and hierarchical zeolitic supports on the controlled release of methylprednisolone hemisuccinate. J Mater Chem B 45:7996–8004CrossRefGoogle Scholar
- 14.Ortiz-Bustos J, Martín A, Morales V, Sanz R, García-Muñoz RA (2017) Surface-functionalization of mesoporous SBA-15 silica materials for controlled release of methylprednisolone sodium hemisuccinate: influence of functionality type and strategies of incorporation. Micropor Mesopor Mat 240:236–245CrossRefGoogle Scholar
- 28.Taranejoo S, Liu J, Verma P, Hourigan K (2015) A review of the developments of characteristics of PEI derivatives for gene delivery applications. J Appl Polym Sci 42096:1–8Google Scholar
- 33.Moussa F (2018)  Fullerene and derivatives for biomedical applications. In: Nayaran R (ed) Nanobiomaterials. Elsevier, pp 113–136Google Scholar
- 42.Bruschi ML (2015) Strategies to modify the drug release from pharmaceutical systems. Woodhead Publishing, Cambridge, pp 63–86Google Scholar
- 47.Desai D, Sen Karaman D, Prabhakar N, Tadayon S, Duchanoy A, Toivola DM, Rajput S, Näreoja T, Rosenholm JM (2014) Design considerations for mesoporous silica nanoparticulate systems in facilitating biomedical applications. Mesoporous Biomater 1:16–43Google Scholar
- 59.Hoffman R, Benz EJ, Silberstein LE, Heslop H, Weitz J, Anastasi J (2018) Aplastic anemia. In: Hoffman R, Benz E, Heslop H, Weitz J (eds) Hematology: basic principles and practice. Elsevier, Amsterdam. ISBN 978-0-323-35762-3Google Scholar