Synthesis of dual-functionalized poly(vinyl alcohol)/poly(acrylic acid) electrospun nanofibers with enzyme and copper ion for enhancing anti-biofouling activities
- 37 Downloads
The aim of this study was to synthesize dual-functionalized poly(vinyl alcohol) (PVA)/poly(acrylic acid) (PAA) electrospun nanofibers with enzyme and copper ion (Cu(II)) for enhancing anti-biofouling activities. The PVA/PAA nanofibers were successfully synthesized by co-electrospinning (voltage = 17 kV; tip-to-collector distance = 15 cm) and cross-linked by heat treatment. The PVA/PAA nanofibers were functionalized through adsorbing Cu(II) onto the nanofibers to prepare the PVA/PAA-Cu(II) nanofibers. Three proteases (proteinase K, trypsin, and α-chymotrypsin) and a quorum quenching enzyme (acylase I) were tested for biofilm reduction that α-chymotrypsin effectively inhibited the biofilm formation and removed biofilms of Pseudomonas aeruginosa and Staphylococcus aureus. The PVA/PAA nanofibers were dual-functionalized with α-chymotrypsin and Cu(II) to obtain PVA/PAA-Cu(II)-α nanofibers. Degradation tests for extracellular polymeric substances (EPS) extracted from P. aeruginosa indicated that the PVA/PAA-Cu(II)-α nanofibers could degrade the EPS proteins up to 0.26 mg mL−1 for 300 min, which was higher than that of free α-chymotrypsin. For anti-biofouling tests, the log number of planktonic and sessile cells of P. aeruginosa was the lowest in the PVA/PAA-Cu(II)-α nanofibers. The anti-biofouling activities of the PVA/PAA-Cu(II)-α nanofibers could be attributed to the effects of both Cu(II) (killing planktonic and sessile cells) and α-chymotrypsin (degrading the EPS protein in biofilm).
This work was supported by the National Research Foundation of Korea, funded by the Ministry of Education, Republic of Korea (Grant number 2017-081271).
- 1.Cloete TE, de Kwaadsteniet M, Botes M, Lopez-Romero JM, Hierrezuelo-Leon J, Cakmakci M, Koyuncu I, Garrido-Perez E (2010) Nanotechnology in water treatment applications. Caister Academic Press, NorfolkGoogle Scholar
- 4.Stewart PS, McFeters GA, Huang CT (2000) Biofilm control by antimicrobial agents. In: Bryers JD (ed) Biofilms II: process analysis and application. Wiley, New YorkGoogle Scholar
- 6.Molobela P, Cloete TE, Beukes M (2010) Protease and amylase enzymes for biofilm removal and degradation of extracellular polymeric substances (EPS) produced by Pseudomonas fluorescens bacteria. Afr J Microbiol Res 4:1515–1524Google Scholar
- 23.Thurman RB, Gerba CP, Bitton G (1989) The molecular mechanisms of copper and silver ion disinfection of bacteria and viruses. Crit Rev Environ Sci Technol 18:295–315Google Scholar
- 28.Xiao SL, Shen MW, Hui M, Guo R, Zhu MF, Wang SY, Shi XY (2010) Fabrication of water-stable electrospun polyacrylic acid-based nanofibrous mats for removal of copper (II) ions in aqueous solution. J Appl Polym Sci 116:2409–2417Google Scholar
- 30.Zmantar T, Kouidhi B, Miladi H, Mahdouani K, Bakhrouf A (2010) A microtiter plate assay for Staphylococcus aureus biofilm quantification at various pH levels and hydrogen peroxide supplementation. New Microbiol 33:137–145Google Scholar
- 31.O’Toole GA, Pratt LA, Watnick PI, Newman DK, Weaver VB, Kolter R (1999) Genetic approaches to study of biofilms. Academic Press San Diego, CaliforniaGoogle Scholar