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Journal of Materials Science

, Volume 54, Issue 13, pp 10055–10064 | Cite as

A polymer dots fluorescent sensor for detection of alkaline phosphatase activity and inhibitor evaluation

  • Jimei Ma
  • Xin Cheng
  • Fangfang Peng
  • Na Zhang
  • Ruifeng Li
  • Linhao Sun
  • Zi-Long Li
  • Hong JiangEmail author
Polymers
  • 35 Downloads

Abstract

Development of a highly sensitive and selective detection method for alkaline phosphatase (ALP) is meaningful in food safety and clinic diagnosis. In this study, we have developed a new fluorescent sensor for ALP activity detection with polymer dots (PDs) based on inner filter effect. The high quantum yield PDs are prepared by 1,4-benzoquinone and ethylenediamine via addition reaction and autopolymerization in aqueous phase under mild conditions. This PDs sensor technology is an effective turn-off fluorescence strategy based on inner filter effect between PDs and p-nitrophenol generated from the ALP enzymatic substrate p-nitrophenylphosphate. The fluorescent assay demonstrates a good linear relationship within 0.1–20 U/L (R2 = 0.998) and a low detection limit of 0.01 U/L. The analysis results of serum samples and inhibitor screening assay suggest that our proposed sensing approach can be used for ALP activity detection in biochemical applications and for the screening of ALP inhibitors in drug discovery.

Notes

Acknowledgements

This work was supported by the Fundamental Research Funds for the Central Universities, China (2662018JC011), and the National Nature Science Foundation of China (21402056).

Supplementary material

10853_2019_3565_MOESM1_ESM.docx (76 kb)
Supplementary material 1 (DOCX 76 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry, College of ScienceHuazhong Agricultural UniversityWuhanChina

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