Biomass-codoped carbon dots: efficient fluorescent probes for isocarbophos ultrasensitive detection and for living cells dual-color imaging
- 25 Downloads
Biomass quantum dots, which are green, cheap and easy to prepare, are promising fluorescence probes. In this study, we prepared biomass nitrogen-codoped carbon dots (B-NCdots) through pyrolysis, using natural soybean as the starting material and introduced the B-NCdots as green fluorescent probes for the ultrasensitive detection of organophosphorus pesticides (OPs). The B-NCdots obtained emitted blue fluorescence, which was quenched by Cu(II) ions. Combining this with an acetylcholinesterase-based cascading enzymatic reaction that produces thiocholine, which can able to cause a reaction with the Cu(II) ions and restore fluorescence. However, OPs can inhibit both the activity of acetylcholinesterase and the recovery of fluorescence. Therefore, a fluorescence “off–on–off” method based on the B-NCdots probes was developed to detect isocarbophos (ICP). A wider linear detection range was achieved from 1.0 × 10−9 to 1.0 × 10−4 M (Limit of Detection = 3.0 × 10−10). The method developed was successfully applied to the detection of ICP in broccoli samples. The average recoveries were in the range of 94.0–103.0% with a relative standard deviation of less than 3.4%. More importantly, the B-NCdots–Cu(II) ion fluorescent probes were also used successfully in dual-color imaging of living cells, indicating that the green fluorescent probes have potential applications in agricultural and biomedical fields.
This work is financially supported by Natural Science Foundation of Guangxi Province (Grant No. 2017GXNSFAA198274).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 9.Akbarzade S, Chamsaz M, Rounaghi GH, Ghorbani M (2018) Zero valent Fe-reduced graphene oxide quantum dots as a novel magnetic dispersive solid phase microextraction sorbent for extraction of organophosphorus pesticides in real water and fruit juice samples prior to analysis by gas chromatography-mass spectrometry. Anal Bioanal Chem 410:429–439CrossRefGoogle Scholar
- 22.Wu N, Liu C, Xu D, Liu J, Liu W, Liu H, Zhang J, Xie W, Guo Z (2019) Ultrathin high-performance electromagnetic wave absorbers with facilely fabricated hierarchical porous Co/C crabapples. J Mater Chem C, in press, https://doi.org/10.1039/c8tc04984j
- 28.Zhao Z, An H, Lin J, Feng M, Murugadoss V, Ding T, Liu H, Shao Q, Mai X, Wang N, Gu H, Angaiah S, Guo Z (2019) Progress on the photocatalytic reduction removal of chromium contamination. Chem Rec, in press, https://doi.org/10.1002/tcr.201800153
- 30.Du H, An Y, Zhang X, Wei Y, Hou L, Liu B, Liu H, Zhang J, Wang N, Umar A, Guo Z (2019) Hydroxyapatite (HA) modified nanocoating enhancement on AZ31 Mg alloy by combined surface mechanical attrition treatment and electrochemical deposition approach. J Nanosci Nanotechnol 19:810–818CrossRefGoogle Scholar