Advertisement

Journal of Materials Science

, Volume 54, Issue 9, pp 6815–6825 | Cite as

Full-color carbon dots with multiple red-emission tuning: on/off sensors, in vitro and in vivo multicolor bioimaging

  • Feng Huo
  • Wenfei Liang
  • Yuran Tang
  • Wei ZhangEmail author
  • Xiaohong Liu
  • Desheng Pei
  • Huabin Wang
  • Weijiao Jia
  • Panpan Jia
  • Feng Yang
Chemical routes to materials
  • 29 Downloads

Abstract

Carbon-based luminescent materials have attracted much attention of many researchers in recent years for its better optical properties and biological applications. In this paper, we report the full-color CDs (from blue to red) with better quality red emissive by a facile and efficient synthesis route. The CDs show multiple red-emission tuning, unique nature of up-conversion photoluminescent under lower excitation and single-particle photoluminescent. The luminescence mechanism was also explored preliminarily. Especially, the results demonstrate that full-color CDs with multiple red wavelength tuning were achieved successfully by using only one precursor and this phenomenon was reasonably explored by using solvatochromism mechanism instead of only excitation-dependent or emission tuning. Moreover, the multicolor FL emission of CDs is caused by single particles rather than a mixture with diverse photoluminescent, which can be expanded to precision manufacture field on luminance, but not just limited to multidimensions biosensing. In addition, UCPL under lower excitation implies the more important potential applications in surface layer bioimaging, microarray detection and light activation technology in vivo and in vitro. The full-color CDs show high quantum yield (73.89%) and superior anti-photobleaching. Multicolor bioimaging, in vitro and in vivo, indicates its low toxicity in Hela cells and zebrafish. Besides, the as-prepared CDs were successfully used for multidimensions sensing, as Fe3+ metal ion sensing (on/off).

Notes

Acknowledgements

This work was supported by research funding from the National Natural Science Foundation of China (Grant No. 61575196), Major Project of Science & Technology Department in Sichan Province (2016JY0168), Youth Innovation Promotion Association of CAS (2015316), the National High Technology Research and Development Program of China (863 Program 2015AA021107), Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing (No. CSTCCXLJRC201714 to D.S.P.).

Compliance with ethical standards

Conflict of interest

All authors declare that there is no conflict of interest.

Supplementary material

10853_2019_3370_MOESM1_ESM.docx (5.1 mb)
Supplementary material 1 (DOCX 5185 kb)

References

  1. 1.
    Sheila NB, Gary AB (2010) Lumineszierende Kohlenstoff-Nanopunkte: nanolichtquellen mit zukunft. Angew Chem Int Ed 122:6876–6896Google Scholar
  2. 2.
    Shin HJ, Clair S, Kim Y, Kawai M (2009) Substrate-induced array of quantum dots in a single-walled carbon nanotube. Nat Nanotechnol 4:567–570Google Scholar
  3. 3.
    Zhang J, Cheng F, Li J, Zhu JJ, Lu Y (2016) Fluorescent nanoprobes for sensing and imaging of metal ions: recent advances and future perspectives. Nano Today 11:309–329Google Scholar
  4. 4.
    Hildebrandt N, Spillmann CM, Algar WR et al (2017) Energy transfer with semiconductor quantum dot bioconjugates: a versatile platform for biosensing, energy harvesting, and other developing applications. Chem Rev 117:536–711Google Scholar
  5. 5.
    Leturcq R, Stampfer C, Inderbitzin K et al (2009) Franck–Condon blockade in suspended carbon nanotube quantum dots. Nat Phys 5:327–331Google Scholar
  6. 6.
    Wang Y, Hu R, Lin G, Roy I, Yong KT (2013) Functionalized quantum dots for biosensing and bioimaging and concerns on toxicity. ACS Appl Mater Interfaces 5:2786–2799Google Scholar
  7. 7.
    Xue Q, Zhang H, Zhu M et al (2017) Photoluminescent Ti3C2 MXene quantum dots for multicolor cellular imaging. Adv Mater 29:1604847–1604852Google Scholar
  8. 8.
    Hofmann MS, Glückert JT, Noé J, Bourjau C, Dehmel R, Högele A (2013) Bright, long-lived and coherent excitons in carbon nanotube quantum dots. Nat Nanotechnol 8:502–505Google Scholar
  9. 9.
    Das SK, Liu Y, Yeom S, Kim DY, Richards CI (2014) Single-particle fluorescence intensity fluctuations of carbon nanodots. Nano Lett 14:620–625Google Scholar
  10. 10.
    Qu S, Zhou D, Li D et al (2016) Toward efficient orange emissive carbon nanodots through conjugated sp(2)-domain controlling and surface charges engineering. Adv Mater 28:3516–3521Google Scholar
  11. 11.
    Liu Q, Guo BD, Rao ZY, Zhang BH, Gong JR (2013) Strong two-photon-induced fluorescence from photostable, biocompatible nitrogen-doped graphene quantum dots for cellular and deep-tissue imaging. Nano Lett 13:2436–2441Google Scholar
  12. 12.
    Lee J, Wong D, Velasco JJ et al (2016) Imaging electrostatically confined Dirac fermions in graphene quantum dots. Nat Phys 12:1032–1036Google Scholar
  13. 13.
    Dong YQ, Wang RX, Li H, Shao JW, Chi YW, Lin XM, Chen GN (2012) Polyamine-functionalized carbon quantum dots for chemical sensing. Carbon 50:2810–2815Google Scholar
  14. 14.
    Wang F, Liu X (2014) Multicolor tuning of lanthanide-doped nanoparticles by single wavelength excitation. Acc Chem Res 47:1378–1385Google Scholar
  15. 15.
    Liu R, Wu D, Liu S, Koynov K, Knoll W, Li Q (2009) An aqueous route to multicolor photoluminescent carbon dots using silica spheres as carriers. Angew Chem Int Ed 48:4598–4601Google Scholar
  16. 16.
    Hola K, Zhang Y, Wang Y, Giannelis EP, Zboril R, Rogach AL (2014) Carbon dots—emerging light emitters for bioimaging, cancer therapy and optoelectronics. Nano Today. 9:590–603Google Scholar
  17. 17.
    Stich MIJ, Schaeferling M, Wolfbeis OS (2009) Multicolor fluorescent and permeation-selective microbeads enable simultaneous sensing of pH, oxygen, and temperature. Adv Mater 21:2216–2220Google Scholar
  18. 18.
    Zhu S, Meng Q, Wang L et al (2013) Highly photoluminescent carbon dots for multicolor patterning, sensors, and bioimaging. Angew Chem Int Ed 52:4045–4049Google Scholar
  19. 19.
    Kalytchuk S, Polakova K, Wang Y, Froning JP, Cepe K, Rogach AL, Zboril R (2017) Carbon dot nanothermometry: intracellular photoluminescence lifetime thermal sensing. ACS Nano 11:1432–1442Google Scholar
  20. 20.
    Li W, Zhang Z, Kong B et al (2013) Simple and green synthesis of nitrogen-doped photoluminescent carbonaceous nanospheres for bioimaging. Angew Chem Int Ed 125:8309–8313Google Scholar
  21. 21.
    Chen D, Yang D, Dougherty CA et al (2017) In vivo targeting and positron emission tomography imaging of tumor with intrinsically radioactive metal-organic frameworks nanomaterials. ACS Nano 11:4315–4327Google Scholar
  22. 22.
    Lei Y, Tang J, Shi H et al (2016) Nature-inspired smart DNA nanodoctor for activatable in vivo cancer imaging and in situ drug release based on recognition-triggered assembly of split aptamer. Anal Chem 88:11699–11706Google Scholar
  23. 23.
    Zheng M, Liu S, Li J et al (2014) Integrating oxaliplatin with highly luminescent carbon dots: an unprecedented theranostic agent for personalized medicine. Adv Mater 26:3554–3560Google Scholar
  24. 24.
    Chen Q, Xu L, Liang C, Wang C, Peng R, Liu Z (2016) Photothermal therapy with immune-adjuvant nanoparticles together with checkpoint blockade for effective cancer immunotherapy. Nat Commun 7:13193–13202Google Scholar
  25. 25.
    Zhang SC, Kang LX, Wang X et al (2017) Arrays of horizontal carbon nanotubes of controlled chirality grown using designed catalysts. Nature 543:234–238Google Scholar
  26. 26.
    Dong Y, Pang H, Yang HB et al (2013) Carbon-based dots co-doped with nitrogen and sulfur for high quantum yield and excitation-independent emission. Angew Chem Int Ed 125:7954–7958Google Scholar
  27. 27.
    Pan LL, Sun S, Zhang A et al (2015) Truly fluorescent excitation-dependent carbon dots and their applications in multicolor cellular imaging and multidimensional sensing. Adv Mater 27:7782–7787Google Scholar
  28. 28.
    Alexander PD, Mariia OD (2016) The origin of emissive states of carbon nanoparticles derived from ensemble-averaged and single-molecular studies. Nanoscale 8:14057–14069Google Scholar
  29. 29.
    Alice S, Michela G, Gianpiero B et al (2018) Disentangling size effects and spectral inhomogeneity in carbon nanodots by ultrafast dynamical hole-burning. Nanoscale 10:15317–15323Google Scholar
  30. 30.
    Yu K, Lee JM, Kim J, Kim G et al (2014) Semiconducting polymers with nanocrystallites interconnected via boron-doped carbon nanotubes. Nano Lett 14:7100–7106Google Scholar
  31. 31.
    Jiang K, Sun S, Zhang L, Lu Y, Wu A, Cai C, Lin H (2015) Red, green, and blue luminescence by carbon dots: full-color emission tuning and multicolor cellular imaging. Angew Chem Int Ed 54:5360–5363Google Scholar
  32. 32.
    Sciortino A, Marino E, Dam B, Schall P, Cannas M, Messina F (2016) Solvatochromism unravels the emission mechanism of carbon nanodots. J Phys Chem Lett 7:3419–3423Google Scholar
  33. 33.
    Qu S, Liu XY, Guo XY, Chu MH, Zhang LG, Shen DZ (2014) Amplified spontaneous green emission and lasing emission from carbon nanoparticles. Adv Funct Mater 24:2689–2695Google Scholar
  34. 34.
    Qu S, Wang XY, Lu QP, Liu XY, Wang LJ (2012) A biocompatible fluorescent ink based on water-soluble luminescent carbon nanodots. Angew Chem Int Ed 51:12215–12218Google Scholar
  35. 35.
    Wang J, Wang CF, Chen S (2012) Amphiphilic egg-derived carbon dots: rapid plasma fabrication, pyrolysis process, and multicolor printing patterns. Angew Chem Int Ed 51:9297–9301Google Scholar
  36. 36.
    Dong YQ, Shao JW, Chen CQ, Li H, Wang RX, Chi YW, Lin XM, Chen GN (2012) Blue luminescent graphene quantum dots and graphene oxide prepared by tuning the carbonization degree of citric acid. Carbon 50:4738–4743Google Scholar
  37. 37.
    Kong B, Zhu A, Ding C, Zhao X, Li B, Tian Y (2012) Carbon dot-based inorganic–organic nanosystem for two-photon imaging and biosensing of pH variation in living cells and tissues. Adv Mater 24:5844–5848Google Scholar
  38. 38.
    Kwon OS, Park SJ, Lee JS et al (2012) Multidimensional conducting polymer nanotubes for ultrasensitive chemical nerve agent sensing. Nano Lett 12:2797–2802Google Scholar
  39. 39.
    Chen K, Shu Q, Schmittel M (2015) Design strategies for lab-on-a-molecule probes and orthogonal sensing. Chem Soc Rev 44:136–160Google Scholar
  40. 40.
    Xie SL, Bian WP, Wang C, Junaid M, Zou JX, Pei DS (2016) A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish. Sci Rep 6:34555–34564Google Scholar
  41. 41.
    Xu HB, Zhou SH, Xiao LL, Wang HH, Li SZ, Yuan QH (2015) Fabrication of a nitrogen-doped graphene quantum dot from MOF-derived porous carbon and its application for highly selective fluorescence detection of Fe3+. J Mater Chem C 3:291–297Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Chemistry and Chemical Engineering, Analytical Testing Center, Institute of Micro/Nano Intelligent SensingNeijiang Normal UniversityNeijiangPeople’s Republic of China
  2. 2.Chongqing Institute of Green and Intelligent TechnologyChinese Academy of SciencesChongqingPeople’s Republic of China
  3. 3.Chongqing Youth Vocational & Technical CollegeChongqingPeople’s Republic of China
  4. 4.Superconductivity and New Energy R&D Center, Key Laboratory of Advanced Technology of Materials (Ministry of Education of China)Southwest Jiaotong UniversityChengduPeople’s Republic of China

Personalised recommendations