Si, N-codoped carbon dots: preparation and application in iron overload diagnosis
- 24 Downloads
Heteroatom doping is a straightforward and smart strategy to improve the fluorescence efficiency of carbon dots (CDs). We synthesized the Si, N-codoped CDs (SiNCDs) with high quantum yield up to 29.7% through one-step hydrothermal method. The linear range for Fe3+ was between 0 and 200 μM, and the limit of detection was about 5 μM, which presented potential for Fe quantification in serum to diagnose Fe overload. In addition, the SiNCDs demonstrated good selectivity to Fe3+ among high concentrations of metal ions, amino acids and H2O2, so there is no need to mix additional reagents as the colorimetric method does in clinic, making SiNCDs more competitive in clinical application. Furthermore, we explored the practicability of SiNCDs by detecting Fe in serum from five healthy volunteers and three patients suffering Fe overload. The recovery rate was from 87.1 to 113.6%, which confirmed the application prospect of SiNCDs in clinical diagnostics.
This work was supported by the National Natural Science Foundation of China (81701789), Medical Research Foundation in Guangdong Province (A2016079) and Natural Science Foundation of Guangdong Province (2017A030310361).
- 2.Netz DJA, Stith CM, Stumpfig M, Kopf G, Vogel D, Genau HM, Stodola JL, Lill R, Burgers PMJ, Pierik AJ (2012) Eukaryotic DNA polymerases require an iron-sulfur cluster for the formation of active complexes Nat. Chem Biol 8:125Google Scholar
- 5.Kohgo Y, Niitsu Y, Kondo H, Kato J, Tsushima N, Sasaki K, Hirayama M, Numata T, Nishisato T, Urushizaki I (1987) Serum transferrin receptor as a new index of erythropoiesis. Blood 70:1955Google Scholar
- 6.Prieto J, Barry M, Sherlock S (1975) Serum ferritin in patients with iron overload and with acute and chronic liver diseases. Gastroenterology 68:525Google Scholar
- 7.Stevens RG, Graubard BI, Micozzi MS, Neriishi K, Blumberg BS (1994) Moderate elevation of body iron level and increased risk of cancer occurrence and death Int. J Cancer 56:364Google Scholar
- 8.Serum Iron Archived 2006-10-28 at the Wayback Machine. University of Illinois Medical Center. https://en.wikipedia.org/wiki/Serum_iron#cite_ref-uimc_1-2. Accessed 6 July 2006
- 11.Forman DT, Vye MV (1980) Immunoradiometric serum ferritin concentration compared with stainable bone-marrow iron as indices to iron stores. Clin Chem 26:145Google Scholar
- 12.Dupuy AM, Debarge L, Poulain M, Badiou S, Ross M, Cristol JP (2009) Determination of serum ferritin using immunoturbidimetry or chemiluminescent detection in comparison with radioimmunoassay a compendium of a methodological juxtaposition. Clin Lab 55:207Google Scholar
- 13.Ceriotti F, Ceriotti G (1980) Improved direct specific determination of serum iron and total iron-binding capacity. Clin Chem 26:327Google Scholar
- 26.Moulder JF, Stickle WF, Sobel PE, Bomben KD (1992) Handbook of X-ray photoelectron spectroscopy-a reference book of standard spectra for identification and interpretation of XPS data, 2nd edn. Perkin-Elmer Corporation, Eden PrairieGoogle Scholar
- 28.NCFCL Standards (1998) Determination of serum iron and total iron-binding capacity; approved standard NCCLS Publ. H17-A 48Google Scholar