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Journal of Materials Science

, Volume 54, Issue 7, pp 5381–5398 | Cite as

Effect of hexaammineruthenium chloride and/or horseradish peroxidase on the performance of hydrogen peroxide (bio)sensors: a comparative study

  • Dilek Söğüt Özdemir
  • Ceren Kaçar
  • Berna Dalkıran
  • Semahat KüçükkolbaşıEmail author
  • Pınar Esra Erden
  • Esma Kılıç
Chemical routes to materials
  • 112 Downloads

Abstract

A comparison of the performances of three hydrogen peroxide (bio)sensors, based on the use of modified glassy carbon electrodes (GCE), is reported. GCE was modified with carboxylated carbon nanotubes (c-MWCNT), graphene (GR), titanium dioxide nanoparticles (TiO2) and hexaammineruthenium chloride (RUT) for the sensor design. In biosensor construction, coupling agents N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide and N-hydroxyl succinimide were used for the immobilization of horseradish peroxidase (HRP) onto TiO2–c-MWCNT–GR–RUT and TiO2–c-MWCNT–GR modified GCEs. The modified electrodes were characterized by scanning electron microscopy, atomic force microscopy, cyclic voltammetry and electrochemical impedance spectroscopy methods. Electrode composition and critical working conditions such as pH and applied potential were optimized. TiO2–c-MWCNT–GR–RUT/GCE and HRP/TiO2–c-MWCNT–GR–RUT/GCE exhibited better analytical performance than HRP/TiO2–c-MWCNT–GR/GCE in terms of detection limit and sensitivity. Moreover, TiO2–c-MWCNT–GR–RUT/GCE sensor showed a sensitivity for H2O2 reduction 1.96 times higher than achieved with HRP/TiO2–c-MWCNT–GR/GCE configuration. The (bio)sensors were also applied to the determination of H2O2 in a disinfector sample, and satisfied results were obtained.

Notes

Acknowledgements

This work was supported by the Research Foundation of Selcuk University (No: 16201004).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Chen S, Yuan R, Chai Y, Hu F (2013) Electrochemical sensing of hydrogen peroxide using metal nanoparticles: a review. Microchim Acta 180(1–2):15–32Google Scholar
  2. 2.
    Chen X, Guo C, Kong J (2012) Oxidative stress in neurodegenerative diseases. Neural Regen Res 7(5):376–385Google Scholar
  3. 3.
    Wang Q, Li M, Szunerits S, Boukherroub R (2014) Environmentally friendly reduction of graphene oxide using tyrosine for nonenzymatic amperometric H2O2 detection. Electroanal 26(1):156–163Google Scholar
  4. 4.
    Çelik Kazıcı H, Caglar A, Aydogmus T, Aktas N, Kivrak H (2018) Microstructured prealloyed Titanium–Nickel powder as a novel nonenzymatic hydrogen peroxide sensor. J Colloid Interface Sci 530:353–360Google Scholar
  5. 5.
    Hong J, Maguhn J, Freitag D, Kettrup A (1998) Determination of H2O2 and organic peroxides by high-performance liquid chromatography with post-column UV irradiation, derivatization and fluorescence detection. Fresenius J Anal Chem 361:124–128Google Scholar
  6. 6.
    Towne V, Will M, Oswald B, Zhao Q (2014) Complexities in horseradish peroxidase-catalyzed oxidation of dihydroxyphenoxazine derivatives: appropriate ranges for pH values and hydrogen peroxide concentrations in quantitative analysis. Anal Biochem 334(2):290–296Google Scholar
  7. 7.
    Chen W, Li B, Xu C, Wang L (2009) Chemiluminescence flow biosensor for hydrogen peroxide using DNAzyme immobilized on eggshell membrane as a thermally stable biocatalyst. Biosens Bioelectron 24(8):2534–2540Google Scholar
  8. 8.
    Hurdis EC, Romeyn H (1954) Accuracy of determination of hydrogen peroxide by cerate oxidimetry. Anal Chem 26(2):320–325Google Scholar
  9. 9.
    Nogueira RFP, Oliveira MC, Paterlini WC (2005) Simple and fast spectrophotometric determination of H2O2 in photo-Fenton reactions using metavanadate. Talanta 66(1):86–91Google Scholar
  10. 10.
    Chen W, Cai S, Ren QQ, Wen W, Zhao YD (2012) Recent advances in electrochemical sensing for hydrogen peroxide: a review. Analyst 137:49–58Google Scholar
  11. 11.
    Wang L, Xu M, Xie Y, Song Y, Wang L (2018) A nonenzymatic electrochemical H2O2 sensor based on macroporous carbon/polymer foam/PtNPs electrode. J Mater Sci 53:10946–10954.  https://doi.org/10.1007/s10853-018-2386-1 Google Scholar
  12. 12.
    Sarika C, Shivakumar MS, Krishnamurthy G, Murthy BN, Lekshmi IC (2017) A novel amperometric catechol biosensor based on a-Fe2O3 nanocrystals-modified carbon paste electrode. Artif Cell Nanomed Biotechnol 45(3):625–634Google Scholar
  13. 13.
    Zhang R, Chen W (2017) Recent advances in graphene-based nanomaterials for fabricating electrochemical hydrogen peroxide sensors. Biosens Bioelectron 89:249–268Google Scholar
  14. 14.
    Scandurra G, Arena A, Ciofi C, Saitta G (2013) Electrical characterization and hydrogen peroxide sensing properties of gold/nafion: polypyrrole/MWCNTs electrochemical devices. Sensors 13:3878–3888Google Scholar
  15. 15.
    Yang J, Xiang H, Shuai L, Gunasekaran S (2011) A sensitive enzymeless hydrogen-peroxide sensor based on epitaxially-grown Fe3O4 thin film. Anal Chim Acta 708(1–2):44–51Google Scholar
  16. 16.
    Zhang X, Li L, Peng X, Chen R, Huo K, Chu PK (2013) Non-enzymatic hydrogen peroxide photoelectrochemical sensor based on WO3 decorated core–shell TiC/C nanofibers electrode. Electrochim Acta 108:491–496Google Scholar
  17. 17.
    Chen X, Wu G, Cai Z, Oyama M, Chen X (2014) Advances in enzyme-free electrochemical sensors for hydrogen peroxide, glucose, and uric acid. Microchim Acta 181(7–8):689–705Google Scholar
  18. 18.
    Wang H, Wang H, Li T, Ma J, Li K, Zuo X (2017) Silver nanoparticles selectively deposited on graphene-colloidal carbon sphere composites and their application for hydrogen peroxide sensing. Sens Actuators B Chem 239:1205–1212Google Scholar
  19. 19.
    Benedet J, Lu D, Cizek K, Belle JL, Wang J (2009) Amperometric sensing of hydrogen peroxide vapor for security screening. Anal Bioanal Chem 395(2):371–376Google Scholar
  20. 20.
    Lu D, Zhang Y, Lin S, Wang L, Wang C (2013) Synthesis of PtAu bimetallic nanoparticles on graphene–carbon nanotube hybrid nanomaterials for nonenzymatic hydrogen peroxide sensor. Talanta 112:111–116Google Scholar
  21. 21.
    Han Y, Zheng J, Dong S (2013) A novel nonenzymatic hydrogen peroxide sensor based on Ag–MnO2–MWCNTs nanocomposites. Electrochim Acta 90:35–43Google Scholar
  22. 22.
    Kaçar C, Erden PE, Pekyardımcı Ş, Kılıç E (2013) An Fe3O4-nanoparticles-based amperometric biosensor for creatine determination. Artif Cell Nanomed Biotechnol 41:2–7Google Scholar
  23. 23.
    Li J, Hu H, Li H, Yao C (2017) Recent developments in electrochemical sensors based on nanomaterials for determining glucose and its byproduct H2O2. J Mater Sci 52:10455–10469.  https://doi.org/10.1007/s10853-017-1221-4 Google Scholar
  24. 24.
    Yang W, Ratinac KR, Ringer SP, Thordarson P, Gooding JJ, Braet F (2010) Carbon nanomaterials in biosensors: should you use nanotubes or graphene? Angew Chem Int Ed 49(12):2114–2138Google Scholar
  25. 25.
    Shi X, Gu W, Li B, Chen N, Zhao K, Xian Y (2014) Enzymatic biosensors based on the use of metal oxide nanoparticles. Microchim Acta 181(1–2):1–22Google Scholar
  26. 26.
    Zhu J, Liu X, Wang X, Huo X, Yan R (2015) Preparation of polyaniline–TiO2 nanotube composite for the development of electrochemical biosensors. Sens Actuators B Chem 221:450–457Google Scholar
  27. 27.
    Kaçar C, Erden PE, Kılıç E (2017) Graphene/poly (vinylferrocene) composite based amperometric biosensor for l-lysine determination. Electroanal 29(9):2114–2124Google Scholar
  28. 28.
    Lu Q, Dong X, Li LJ, Hu X (2010) Direct electrochemistry-based hydrogen peroxide biosensor formed from single-layer graphene nanoplatelet–enzyme composite film. Talanta 82(4):1344–1348Google Scholar
  29. 29.
    Qian L, Yang X (2006) Composite film of carbon nanotubes and chitosan for preparation of amperometric hydrogen peroxide biosensor. Talanta 68(3):721–727Google Scholar
  30. 30.
    Salimi A, Hallaj R, Soltanian S, Mamkhezri H (2007) Nanomolar detection of hydrogen peroxide on glassy carbon electrode modified with electrodeposited cobalt oxide nanoparticles. Anal Chim Acta 594(1):24–31Google Scholar
  31. 31.
    Wang L, Wang E (2004) A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized on colloidal Au modified ITO electrode. Electrochem Commun 6(2):225–229Google Scholar
  32. 32.
    Niu X, Yang W, Guo H, Ren J, Gao J (2013) Highly sensitive and selective dopamine biosensor based on 3, 4, 9, 10-perylene tetracarboxylic acid functionalized graphene sheets/multi-wall carbon nanotubes/ionic liquid composite film modified electrode. Biosens Bioelectron 41:225–231Google Scholar
  33. 33.
    Kaçar C, Dalkiran B, Erden PE, Kılıç E (2014) An amperometric hydrogen peroxide biosensor based on Co3O4 nanoparticles and multiwalled carbon nanotube modified glassy carbon electrode. Appl Surf Sci 311:139–146Google Scholar
  34. 34.
    Dalkıran B, Erden PE, Kılıç E (2017) Graphene and tricobalt tetraoxide nanoparticles based biosensor for electrochemical glutamate sensing. Artif Cell Nanomed Biotechnol 45(2):340–348Google Scholar
  35. 35.
    Hwa KY, Subramani B (2014) Synthesis of zinc oxide nanoparticles on graphene–carbon nanotube hybrid for glucose biosensor applications. Biosens Bioelectron 62:127–133Google Scholar
  36. 36.
    Jin E, Bian X, Lu X, Wang C (2012) Fabrication of multiwalled carbon nanotubes/polypyrrole/Prussian blue ternary composite nanofibers and their application for enzymeless hydrogen peroxide detection. J Mater Sci 47:4326–4331.  https://doi.org/10.1007/s10853-012-6283-8 Google Scholar
  37. 37.
    Erden PE, Kaçar C, Öztürk F, Kılıç E (2015) Amperometric uric acid biosensor based on poly (vinylferrocene)-gelatin-carboxylated multiwalled carbon nanotube modified glassy carbon electrode. Talanta 134:488–495Google Scholar
  38. 38.
    Niyomdecha S, Limbut W, Numnuam A, Asawatreratanakul P, Kanatharana P, Thavarungkul P (2017) A novel BOD biosensor based on entrapped activated sludge in a porous chitosan-albumin cryogel incorporated with graphene and methylene blue. Sensor Actuators B Chem 241:473–481Google Scholar
  39. 39.
    Erden PE, Zeybek B, Pekyardimcı Ş, Kiliç E (2013) Amperometric carbon paste enzyme electrodes with Fe3O4 nanoparticles and 1, 4-benzoquinone for glucose determination. Artif Cell Nanomed Biotechnol 41(3):165–171Google Scholar
  40. 40.
    Chaubey A, Malhotra B (2002) Mediated biosensors. Biosens Bioelectron 17(6–7):441–456Google Scholar
  41. 41.
    Astier Y, Canters GW, Davis JJ, Hill HA, Verbeet MP, Wijma HJ (2005) Sensing nitrite through a pseudoazurin–nitrite reductase electron transfer relay. Chem Phys Chem 26(6):1114–1120Google Scholar
  42. 42.
    Loew N, Tsugawa W, Nagae D, Kojima K, Sode K (2017) Mediator preference of two different fad-dependent glucose dehydrogenases employed in disposable enzyme glucose sensors. Sensors 17(11):2636Google Scholar
  43. 43.
    Kamyabi MA, Hajari NJ (2017) Low potential and non-enzymatic hydrogen peroxide sensor based on copper oxide nanoparticle on activated pencil graphite electrode. J Brazil Chem Soc 28(5):808–818Google Scholar
  44. 44.
    Zhang Y, Yuan R, Chai Y, Xiang Y, Hong C, Ran X (2010) An amperometric hydrogen peroxide biosensor based on the immobilization of HRP on multi-walled carbon nanotubes/electro-copolymerized nano-Pt-poly (neutral red) composite membrane. Biochem Eng J 51(3):102–109Google Scholar
  45. 45.
    Campuzano S, Serra B, Pedrero M, de Villena FJM, Pingarrón JM (2003) Amperometric flow-injection determination of phenolic compounds at self-assembled monolayer-based tyrosinase biosensors. Anal Chim Acta 494(1–2):187–197Google Scholar
  46. 46.
    Yang L, Ren X, Tang F, Zhang L (2009) A practical glucose biosensor based on Fe3O4 nanoparticles and chitosan/nafion composite film. Biosens Bioelectron 25:889–895Google Scholar
  47. 47.
    Yao H, Hu NJ (2010) pH-switchable bioelectrocatalysis of hydrogen peroxide on layer-by-layer films assembled by concanavalin A and horseradish peroxidase with electroactive mediator in solution. J Phys Chem B 114(9):3380–3386Google Scholar
  48. 48.
    Metzker G, de Aguiar I, Martins SC, Schultz MS, Vasconcellos LCG, Franco DW (2014) Electrochemical and chemical aspects of ruthenium (II) and (III) ammines in basic solution: the role of the ruthenium (IV) species. Inorg Chim Acta 416:142–146Google Scholar
  49. 49.
    Dervisevic M, Custiuc E, Çevik E, Şenel M (2015) Construction of novel xanthine biosensor by using polymeric mediator/MWCNT nanocomposite layer for fish freshness detection. Food Chem 181:277–283Google Scholar
  50. 50.
    Liu S, Ju H (2003) Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode. Biosens Bioelectron 19(3):177–183Google Scholar
  51. 51.
    Çelik AC, Öztürk F, Erden PE, Kaçar C, Kılıç E (2015) Amperometric lactate biosensor based on carbon paste electrode modified with benzo [c] cinnoline and multiwalled carbon nanotubes. Electroanal 27(12):2820–2828Google Scholar
  52. 52.
    Laviron E (1979) General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems. J Electroanal Chem Interfacial Electrochem 101(1):19–28Google Scholar
  53. 53.
    Ruzgas T, Gorton L, Emneus J, Marko-Varga G (1995) Kinetic models of horseradish peroxidase action on a graphite electrode. J Electroanal Chem 391(1–2):41–49Google Scholar
  54. 54.
    Devi R, Batra B, Lata S, Yadav S, Pundir CS (2015) A method for determination of xanthine in meat by amperometric biosensor based on silver nanoparticles/cysteine modified Au electrode. Process Biochem 48(2):242–249Google Scholar
  55. 55.
    Rocchitta G, Spanu A, Babudieri S, Latte G, Madeddu G, Galleri G, Nuvoli S, Bagella P, Demartis MI, Fiore V, Manetti R, Serra PA (2016) Enzyme biosensors for biomedical applications: strategies for safeguarding analytical performances in biological fluids. Sens (Basel) 6:780Google Scholar
  56. 56.
    Korkut S, Keskinler B, Erhan E (2008) An amperometric biosensor based on multiwalled carbon nanotube-poly(pyrrole)-horseradish peroxidase nanobiocomposite film for determination of phenol derivatives. Talanta 76:1147–1152Google Scholar
  57. 57.
    Abdelwahab AA, Shim YB (2014) Nonenzymatic H2O2 sensing based on silver nanoparticles capped polyterthiophene/MWCNT nanocomposite. Sens Actuators B Chem 201:51–58Google Scholar
  58. 58.
    Wang MY, Shen T, Wang M, Zhang DE, Tong ZW, Chen J (2014) One-pot synthesis of α-Fe2O3 nanoparticles-decorated reduced graphene oxide for efficient nonenzymatic H2O2 biosensor. Sens Actuators B Chem 190:645–650Google Scholar
  59. 59.
    Xin Y, Fu-bing X, Hong-wei L, Feng W, Di-zhao C, Zhao-yang W (2013) A novel H2O2 biosensor based on Fe3O4–Au magnetic nanoparticles coated horseradish peroxidase and graphene sheets–Nafion film modified screen-printed carbon electrode. Electrochim Acta 109:750–755Google Scholar
  60. 60.
    Guler M, Turkoglu V, Basi Z (2017) Determination of malation, methidathion, and chlorpyrifos ethyl pesticides using acetylcholinesterase biosensor based on Nafion/Ag@ rGO-NH2 nanocomposites. Electrochim Acta 240:129–135Google Scholar
  61. 61.
    Apetrei C, Rodríguez-Méndez ML, De Saja JA (2011) Amperometric tyrosinase based biosensor using an electropolymerized phosphate-doped polypyrrole film as an immobilization support. Application for detection of phenolic compounds. Electrochim Acta 56(24):8919–8925Google Scholar
  62. 62.
    Chen C, Hong X, Xu T, Chen A, Lu L, Gao Y (2016) Hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase onto a poly (aniline-co-N-methylthionine) film. Synth Metal 212:123–130Google Scholar
  63. 63.
    Xiang C, Zou Y, Sun LX, Xu F (2009) Direct electrochemistry and enhanced electrocatalysis of horseradish peroxidase based on flowerlike ZnO–gold nanoparticle–Nafion nanocomposite. Sens Actuators B Chem 136(1):158–162Google Scholar
  64. 64.
    Liu SQ, Ju HX (2002) Renewable reagentless hydrogen peroxide sensor based on direct electron transfer of horseradish peroxidase immobilized on colloidal gold-modified electrode. Anal Biochem 307(1):110–116Google Scholar
  65. 65.
    Kristine FJ, Johnson CR, O’Donnell S, Shepherd RE (1980) Reduction of hydrogen peroxide by ruthenium (II) ammine complexes: the surprisingly identical mechanism for hexaammineruthenium (2+), pentaammine (aquo) ruthenium (2+), and pentaammine (1-methylimidazole) ruthenium (2+). Inorg Chem 19(8):2280–2284Google Scholar
  66. 66.
    Zhao W, Wang H, Qin X, Wang X, Zhao Z, Miao Z, Chen L, Shan M, Fang Y, Chen Q (2009) A novel nonenzymatic hydrogen peroxide sensor based on multi-wall carbon nanotube/silver nanoparticle nanohybrids modified gold electrode. Talanta 80(2):1029–1033Google Scholar
  67. 67.
    Khan MM, Ansari SA, Lee J, Cho MH (2013) Novel Ag@ TiO2 nanocomposite synthesized by electrochemically active biofilm for nonenzymatic hydrogen peroxide sensor. Mat Sci Engi C Mater 33(8):4692–4699Google Scholar

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Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of ScienceSelçuk UniversityKonyaTurkey
  2. 2.Department of Chemistry, Faculty of ScienceAnkara UniversityAnkaraTurkey
  3. 3.Department of Chemistry, Polatlı Faculty of Science and ArtsAnkara Hacı Bayram Veli UniversityAnkaraTurkey

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