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Bioinformatics and enzymatics investigation of Trametes laccase for optical biosensing application

  • Yang Wang
  • Zhi-Hui Chen
Materials for life sciences
  • 13 Downloads

Abstract

Laccase has recently drawn a considerable attention as a promising tool for optical biosensing application, mainly due to its attractive intrinsic optical properties. The discovery of a laccase with great enzyme stability is the principal step for enzyme-based optical biosensing application in in vitro environments. In this paper, we found that Trametes sp. SQ1 laccase retained more than 150% activity after storage in different conditions for 96 h, and the laccase activity was not affected by freeze–thaw. The reduced form of enzyme showed a new maximal increase in absorbance peak at 400 nm, while the fluorescence intensity of the oxidized form of enzyme was much stronger than that of the reduced enzyme. Therefore, Trametes sp. SQ1 laccase with good stability and optical properties will be a competitive candidate for optical biosensing application. Moreover, bioinformatics analysis on Trametes laccases revealed that the enzymes may be modified by N-glycosylation and have intermolecular disulfide bond for forming functional oligomer, which plays a role in stabilizing enzyme function. The results indicate that Trametes laccases are able to be with good stability. It will provide a guide in searching stable laccases for optical biosensing application.

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31640019, 11674239 and 21601112) and Program for the Top Young Talents of Shanxi Province, China. The authors thank Prof. X. Yang for Trametes sp. SQ01.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. 1.Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of BiotechnologyShanxi UniversityTaiyuanChina
  2. 2.Key Lab of Advanced Transducers and Intelligent Control System, Ministry of Education and Shanxi Province, College of Physics and OptoelectronicsTaiyuan University of TechnologyTaiyuanChina

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