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Journal of Materials Science

, Volume 52, Issue 15, pp 9211–9223 | Cite as

Bioactive glasses functionalized with polyphenols: in vitro interactions with healthy and cancerous osteoblast cells

  • M. Cazzola
  • E. Vernè
  • A. Cochis
  • R. Sorrentino
  • B. Azzimonti
  • E. Prenesti
  • L. Rimondini
  • S. Ferraris
In Honor of Larry Hench

Abstract

Bioactive glasses are widely studied as biomaterials for bone contact applications. In this research work, the opportunity to modify the surface of a bioactive glass with polyphenols (gallic acid, and natural polyphenols extracted from red grape skin and green tea leaves) has been investigated in order to induce a selective anti-tumor activity in vitro. The presence of surface grafted molecules has been optically proved by fluorescence microscopy exploiting their auto-fluorescence. Direct and indirect cytotoxicity assays have been performed with human bone osteosarcoma cells (U2OS) and human fetal pre-osteoblasts (hFOB), as well as the quantification of oxygen and nitrogen reactive species (RONS) engendered from cells in response to the materials. Finally, the DNA damage of U2OS cells upon contact with the bioactive glass has been evaluated in order to verify any selective cytotoxic activity of functionalized materials against cancer cells. Results showed a selective cytotoxic activity of functionalized bioactive glasses toward osteosarcoma cells that was particularly evident when cells were cultivated directly onto glasses surface. Moreover, the presence of grafted polyphenols increased the RONS production and induced a permanent DNA damage on the U2SOS cells while they promote a certain anti-inflammatory action toward hFOB. These preliminary results suggest polyphenols grafted bioactive glasses as promising material for bone substitution in cancer treatment.

Keywords

Polyphenol Gallic Acid Bioactive Glass U2OS Cell hFOB Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Applied Science and Technology, Institute of Materials Physics and EngineeringPolitecnico di TorinoTurinItaly
  2. 2.Department of Health SciencesUniversità del Piemonte Orientale UPONovaraItaly
  3. 3.Department of ChemistryUniversità degli Studi di TorinoTurinItaly

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