Journal of Materials Science

, Volume 51, Issue 9, pp 4262–4273 | Cite as

Laser-assisted fabrication and non-invasive imaging of 3D cell-seeding constructs for bone tissue engineering

  • M. MihailescuEmail author
  • I. A. PaunEmail author
  • M. ZamfirescuEmail author
  • C. R. LuculescuEmail author
  • A. M. Acasandrei
  • M. Dinescu
Original Paper


We report on laser-assisted fabrication and non-invasive imaging of porous 3D cell-seeding constructs (3D-CSCs) for bone tissue engineering. The 3D structures were built by two-photon polymerization-direct writing (2PP_DW) of IP-L780 photopolymer and consist in arrays of vertical microtubes arranged in triangular lattices. The microtubes were tightly, medium, and rarely packed, according to the constants of the triangular lattices of 8, 12, and 24 μm, respectively. The efficiency of the laser-generated 3D-CSCs for new bone formation was assessed in MG63 osteoblast-like cells cultures. High spatial resolution 3D images of the cell-seeded 3D-CSCs were obtained by digital holographic microscopy (DHM). The recorded holograms allowed the simultaneous evaluation of the 3D-CSCs and of the seeded cells, in terms of 3D shapes and dimensions, without intruding into the cells natural environment. The seeded cells, in particular the cells nuclei, conformed to the micro-architectures of the 3D-CSCs. Furthermore, the osteogenic potential of the 3D-CSCs was assessed in terms of cell morphology, viability, and level of mineralization. The microtubes packing density that allowed the seeded osteoblasts to reach the highest level of mineralization was established.


MG63 Cell Triangular Lattice Bone Tissue Engineering Cell Mineralization Object Beam 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the contract UEFISCDI, PN-II-PT-PCCA no. 6/2012, LAPLAS3 no. PN 09 39 (Program Nucleu) 01/2015, and by a grant of the Romanian Authority for Scientific Research and Innovation, CNCS-UEFISCDI, project number PN-II-RU-TE-2014-4-2534 (contract number 97 from 01/10/2015).


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Politehnica UniversityBucharestRomania
  2. 2.Laser DepartmentNational Institute of Lasers, Plasma and Radiation PhysicsMagureleRomania
  3. 3.Center for Advanced Laser Technologies - CETALNational Institute of Lasers, Plasma and Radiation PhysicsMagureleRomania
  4. 4.National Institute of Physics and Nuclear EngineeringMagureleRomania

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