45S5 Bioglass® concentrations modulate the release of vancomycin hydrochloride from gelatin–starch films: evaluation of antibacterial and cytotoxic effects
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The aim of this work was to evaluate the release profile of vancomycin hydrochloride (VC), as well as the degradation, in vitro antistaphylococcal effect and cytotoxicity in MG-63 osteoblast-like cells of gelatin–starch (GS) films added with different concentrations of microparticles of the bioactive glass 45S5 (m-BG). The biomaterials were obtained through the gel-casting method. Four different composites were prepared at four different weight percentages of m-BG: 0, 5, 10, and 15 %. Glutaraldehyde 0.25 wt% (GA) was used as the cross-linker. The composites were characterized by scanning electron microscopy and the in vitro degradation of the films was studied by measuring the water uptake and weight loss. The drug release kinetics was quantified spectrophotometrically. The inhibition zone test and the plate count method were used to evaluate the antibacterial activity of the samples. Three staphylococcus strains were evaluated: Staphylococcus aureus ATCC6538, S. aureus ATCC29213, and Staphylococcus epidermidis ATCC12228. Cytotoxicity effects were evaluated through the MTT assay. The addition of m-BG to GS films showed no effects on the amount of water uptake, but led to an increase in the weight loss over time, even with m-BG content. The release rate of VC was also affected by the increasing concentration of m-BG in the composite films. However, the antibacterial effects of the composites were not improved by this modulation. All composites strongly inhibited staphylococcal cells with similar strength. On the other hand, liquid extracts from the composites resulted in cytotoxic effects on MG-63 osteoblast-like cells due to the presence of GA, but not to the concentration of VC or m-BG.
KeywordsComposite Film Inhibition Zone Bioactive Glass Genipin Water Uptake Capacity
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Conflict of interest
The authors declare that they have no competing interests.
- 6.Ivanova P, Bazaka L, Crawford RJ (eds) (2014) New functional biomaterials for medicine and healthcare, Chapter 2. Woodhead Publishing, Oxford. ISBN 9781782422655Google Scholar
- 10.Foox M, Zilberman M (2015) Drug delivery from gelatin-based system. Expert Opin Drug Deliv 5:1–17Google Scholar
- 15.Leng YG, Huang YQ, Dong CL, Huang MZ (2002) Research of grafting acrylamide on gelatin. Polym Mater Sci Eng 18:93–97Google Scholar
- 23.Phromsopha T, Baimark Y (2014) Preparation of starch/gelatin blend microparticles by a water-in-oil emulsion method for controlled release. Drug Deliv Int J Biomater 2014:829490Google Scholar
- 25.Olalde B, Garmendia N, Sáez-Martínez V, Argarate N, Nooeaid P, Morin F, Boccaccini AR (2013) Multifunctional bioactive glass scaffolds coated with layers of poly(d, l-lactide-co-glycolide) and poly(n-isopropylacrylamide-co-acrylic acid) microgels loaded with vancomycin. Mater Sci Eng C Mater Biol Appl 33:3760–3767CrossRefGoogle Scholar
- 47.Misra SK, Ansari T, Mohn D, Valappil SP, Brunner TJ, Stark WJ, Roy I, Knowles JC, Sibbons PD, Jones EV, Boccaccini AR, Salih V (2010) Effect of nanoparticulate bioactive glass particles on bioactivity and cytocompatibility of poly(3-hydroxybutyrate) composites. J R Soc Interface 7:453–465CrossRefGoogle Scholar
- 49.Boccaccini AR, Maquet V (2003) Bioresorbable and bioactive polymer/bioglass composites with tailored pore structure for tissue engineering applications. Compos Sci Technol 63:2417e29Google Scholar
- 50.Blaker J, Nazhat SN, Maquet V, Boccaccini AR (2011) Long-term in vitro degradation of PDLLA/bioglass bone scaffolds in a cellular simulated body fluid. Acta Bio-mater 7:829e40Google Scholar
- 53.Das Gupta V, Stewart KR, Nohria S (1986) Stability of vancomycin hydrochloride in 5 % dextrose and 0.9 % sodium chloride, injections. Am J Hosp Pharm 43:1729–1731Google Scholar