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45S5 Bioglass® concentrations modulate the release of vancomycin hydrochloride from gelatin–starch films: evaluation of antibacterial and cytotoxic effects

  • In Honor of Larry Hench
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Abstract

The aim of this work was to evaluate the release profile of vancomycin hydrochloride (VC), as well as the degradation, in vitro antistaphylococcal effect and cytotoxicity in MG-63 osteoblast-like cells of gelatin–starch (GS) films added with different concentrations of microparticles of the bioactive glass 45S5 (m-BG). The biomaterials were obtained through the gel-casting method. Four different composites were prepared at four different weight percentages of m-BG: 0, 5, 10, and 15 %. Glutaraldehyde 0.25 wt% (GA) was used as the cross-linker. The composites were characterized by scanning electron microscopy and the in vitro degradation of the films was studied by measuring the water uptake and weight loss. The drug release kinetics was quantified spectrophotometrically. The inhibition zone test and the plate count method were used to evaluate the antibacterial activity of the samples. Three staphylococcus strains were evaluated: Staphylococcus aureus ATCC6538, S. aureus ATCC29213, and Staphylococcus epidermidis ATCC12228. Cytotoxicity effects were evaluated through the MTT assay. The addition of m-BG to GS films showed no effects on the amount of water uptake, but led to an increase in the weight loss over time, even with m-BG content. The release rate of VC was also affected by the increasing concentration of m-BG in the composite films. However, the antibacterial effects of the composites were not improved by this modulation. All composites strongly inhibited staphylococcal cells with similar strength. On the other hand, liquid extracts from the composites resulted in cytotoxic effects on MG-63 osteoblast-like cells due to the presence of GA, but not to the concentration of VC or m-BG.

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Authors and Affiliations

  1. Grupo Interdisciplinario en Materiales- Universidad Católica de Salta (IESIING-UCASAL), Instituto de Tecnologías y Ciencias de Ingeniería-Universidad Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (INTECIN UBA-CONICET), Campo Castañares s/n, Salta, Argentina

    Josefina Rivadeneira & Alejandro A. Gorustovich

  2. Cátedra de Bioquímica Patológica Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), Calle 47 y115, La Plata, Argentina

    Ana Laura Di Virgilio

  3. Instituto de Investigaciones para la Industria Química - Consejo Nacional de Investigaciones Científicas y Técnicas (INIQUI - CONICET), Universidad Nacional de Salta (UNSa), Av. Bolivia 5150, Salta, Argentina

    M. Carina Audisio

  4. Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany

    Aldo R. Boccaccini

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  1. Josefina Rivadeneira
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  2. Ana Laura Di Virgilio
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  3. M. Carina Audisio
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  4. Aldo R. Boccaccini
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  5. Alejandro A. Gorustovich
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Correspondence to Josefina Rivadeneira.

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Rivadeneira, J., Di Virgilio, A.L., Audisio, M.C. et al. 45S5 Bioglass® concentrations modulate the release of vancomycin hydrochloride from gelatin–starch films: evaluation of antibacterial and cytotoxic effects. J Mater Sci 52, 9091–9102 (2017). https://doi.org/10.1007/s10853-016-0512-5

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