Antibacterial efficacy, corrosion resistance, and cytotoxicity studies of copper-substituted carbonated hydroxyapatite coating on titanium substrate
- 1k Downloads
This work elucidated the antibacterial efficacy, corrosion resistance, and cytotoxicity of electroplated copper-substituted hydroxyapatite (CuHAP) coating on titanium (Ti). The fabricated CuHAP coatings were characterized by scanning electron microscopy, energy-dispersive X-ray analysis spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction studies. The CuHAP coating had needle-like apatite crystals, the doping of Cu2+ into HAP reduced porosity, and the coating became denser. The CuHAP crystals were carbonated with a few of Cu2+ incorporation (about 0.80 wt%). The Cu2+ ions were homogenously deposited into HAP films. Potentiodynamic polarisation test revealed that the CuHAP coating provided good barrier characteristics and achieved superior corrosion protection for Ti substrates. The in vitro antibacterial activity of as-prepared CuHAP coating was evaluated against Escherichia coli and was found to be effectively high against bacterial colonization. Bioactivity test conducted by soaking the coatings in simulated body fluid demonstrated that CuHAP coating can quickly induce bone-like apatite nucleation and growth. In vitro biocompatibility tests, MTT, were employed to assess the cytotoxicity of CuHAP coating with osteoblast-like MC3T3-E1 cells. The obtained HAP coating doped with a low content of Cu2+ exhibited good cytocompatibility and had no toxicity toward MC3T3-E1.
KeywordsSimulated Body Fluid Antibacterial Efficacy Electrolytic Deposition Simulated Body Fluid Immersion Cupric Nitrate
This work was supported by the National Basic Research Program of China (“973” Program, No. 2011CB503700) and the outstanding doctoral academic projects of the University of Electronic Science and Technology of China (No. YBXSZC20131042).
- 10.Radovanovic Z, Jokic B, Veljovic D, Dimitrijevic S, Kojic V, Petrovic R et al (2014) Antimicrobial activity and biocompatibility of Ag+- and Cu2+-doped biphasic hydroxyapatite/α-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+-doped hydroxyapatite. Appl Surf Sci 307:513–519CrossRefGoogle Scholar
- 39.Ohsumi Y, Kitamoto K, Anraku Y (1988) Changes induced in the permeability barrier of the yeast plasma membrane by cupric ion. J Bacteriol 170:2676–2682Google Scholar
- 52.Ewald A, Kappel C, Vorndran E, Moseke C, Gelinsky M, Gbureck U (2012) The effect of Cu(II)-loaded brushite scaffolds on growth and activity of osteoblastic cells. J Biomed Mater Res A 100A:2392–2400Google Scholar