Journal of Materials Science

, Volume 50, Issue 4, pp 1688–1700 | Cite as

Antibacterial efficacy, corrosion resistance, and cytotoxicity studies of copper-substituted carbonated hydroxyapatite coating on titanium substrate

  • Yong Huang
  • Xuejiao Zhang
  • Ranlin Zhao
  • Huanhuan Mao
  • Yajing Yan
  • Xiaofeng Pang
Original Paper


This work elucidated the antibacterial efficacy, corrosion resistance, and cytotoxicity of electroplated copper-substituted hydroxyapatite (CuHAP) coating on titanium (Ti). The fabricated CuHAP coatings were characterized by scanning electron microscopy, energy-dispersive X-ray analysis spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction studies. The CuHAP coating had needle-like apatite crystals, the doping of Cu2+ into HAP reduced porosity, and the coating became denser. The CuHAP crystals were carbonated with a few of Cu2+ incorporation (about 0.80 wt%). The Cu2+ ions were homogenously deposited into HAP films. Potentiodynamic polarisation test revealed that the CuHAP coating provided good barrier characteristics and achieved superior corrosion protection for Ti substrates. The in vitro antibacterial activity of as-prepared CuHAP coating was evaluated against Escherichia coli and was found to be effectively high against bacterial colonization. Bioactivity test conducted by soaking the coatings in simulated body fluid demonstrated that CuHAP coating can quickly induce bone-like apatite nucleation and growth. In vitro biocompatibility tests, MTT, were employed to assess the cytotoxicity of CuHAP coating with osteoblast-like MC3T3-E1 cells. The obtained HAP coating doped with a low content of Cu2+ exhibited good cytocompatibility and had no toxicity toward MC3T3-E1.


Simulated Body Fluid Antibacterial Efficacy Electrolytic Deposition Simulated Body Fluid Immersion Cupric Nitrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the National Basic Research Program of China (“973” Program, No. 2011CB503700) and the outstanding doctoral academic projects of the University of Electronic Science and Technology of China (No. YBXSZC20131042).


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Yong Huang
    • 1
    • 2
  • Xuejiao Zhang
    • 1
  • Ranlin Zhao
    • 2
  • Huanhuan Mao
    • 2
  • Yajing Yan
    • 2
  • Xiaofeng Pang
    • 2
    • 3
  1. 1.College of Lab MedicineHebei North UniversityZhangjiakouChina
  2. 2.Institute of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduChina
  3. 3.International Centre for Materials PhysicsChinese Academy of ScienceShenyangChina

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