Journal of Materials Science

, Volume 47, Issue 15, pp 5661–5672 | Cite as

Poly-dl-lactic acid coated Bioglass® scaffolds: toughening effects and osteosarcoma cell proliferation

  • Oana Bretcanu
  • Aldo R. BoccacciniEmail author
  • Vehid SalihEmail author
Syntactic & Composite Foams


The fabrication and in vitro characterisation of 45S5 Bioglass®-derived scaffolds coated with poly-dl-lactic acid (PDLLA) are presented. Two types of scaffolds with different porosities were studied. The scaffold microstructure was analysed by scanning electron microscopy before and after polymer coating. Compressive strength tests were performed in as-fabricated condition and after 28 days of immersion in simulated body fluid. It was found that PDLLA-coated samples have higher compression strength compared with the uncoated samples. Human osteosarcoma cell line (HOS-TE85) was used to determine the biocompatibility of the scaffolds. Cell proliferation and differentiated functions were investigated by AlamarBlue, alkaline phosphatase and osteocalcin assays. The scaffolds were maintained in cell culture for periods of up to 14 days. The combined effects of the scaffold microstructure (porosity, surface roughness and bioreactivity) and the polymeric coating induce favourable HOS cell behaviour. The results confirmed the biocompatibility of Bioglass®/PDLLA composite scaffolds for HOS cell culture and contribute to the understanding of the characteristics of these scaffolds for potential applications in bone tissue engineering.


Simulated Body Fluid Coated Sample Composite Scaffold Osteogenic Medium Simulated Body Fluid Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was funded by the EU IntraEuropean Marie Curie fellowship (No 24248).


  1. 1.
    Alves NM, Leonor IB, Azevedo HS, Reis RL, Mano JF (2010) J Mater Chem 20:2911CrossRefGoogle Scholar
  2. 2.
    Hutmacher DW, Schantz JT, Lam CXF, Tan KC, Lim TC (2007) J Tissue Eng Regen Med 1:245CrossRefGoogle Scholar
  3. 3.
    El-Ghannam AR (2004) J Biomed Mater Res A 69:490CrossRefGoogle Scholar
  4. 4.
    Wu C (2009) Expert Rev Med Devices 6:237CrossRefGoogle Scholar
  5. 5.
    Guarino V, Causa F, Ambrosio L (2007) Expert Rev Med Devices 4:405CrossRefGoogle Scholar
  6. 6.
    Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR (2006) Biomaterials 27:3413CrossRefGoogle Scholar
  7. 7.
    Yunos DM, Bretcanu O, Boccaccini AR (2008) J Mater Sci 43:4433. doi: 10.1007/s10853-008-2552-y CrossRefGoogle Scholar
  8. 8.
    Chen QZ, Thompson ID, Boccaccini AR (2006) Biomaterials 27:2414CrossRefGoogle Scholar
  9. 9.
    Chen QZ, Boccaccini AR (2006) J Biomed Mater Res 77A:445CrossRefGoogle Scholar
  10. 10.
    Bretcanu O, Misra S, Roy I, Renghini C, Fiori F, Boccaccini AR, Salih V (2009) J Tissue Regen Med 3:139CrossRefGoogle Scholar
  11. 11.
    Wu C, Ramaswamy Y, Boughton P, Zreiqat H (2008) Acta Biomater 4:343CrossRefGoogle Scholar
  12. 12.
    Miao X, Tan LP, Tan LS, Huang X (2007) Mater Sci Eng 27:274CrossRefGoogle Scholar
  13. 13.
    Kim HW, Knowles JC, Kim HE (2004) J Biomed Mater Res 70B:240CrossRefGoogle Scholar
  14. 14.
    Chen QZ, Efthymiou A, Salih V, Boccaccini AR (2008) J Biomed Mater Res 84A:1049CrossRefGoogle Scholar
  15. 15.
    Clover J, Gowen M (1994) Bone 15:585CrossRefGoogle Scholar
  16. 16.
    Kokubo T, Ito S, Huang ZT et al (1990) J Biomed Mater Res 24:331CrossRefGoogle Scholar
  17. 17.
  18. 18.
  19. 19.
  20. 20.
  21. 21.
    Boyan BD et al (1996) Biomaterials 17:137CrossRefGoogle Scholar
  22. 22.
    Hench LL, Wilson J (1999) An introduction to bioceramics. World Scientific Publishing, SingaporeGoogle Scholar
  23. 23.
    Peroglio M, Gremillard L, Chevalier J, Chazeau L, Gauthier C, Hamaide T (2007) J Eur Ceram Soc 27:2679CrossRefGoogle Scholar
  24. 24.
    Blaker JJ, Nazhat SN, Maquet V, Boccaccini AR (2011) Acta Biomater 7:829CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.School of Mechanical and Systems EngineeringNewcastle UniversityNewcastle upon TyneUK
  2. 2.Institute of BiomaterialsUniversity of Erlangen-NurembergErlangenGermany
  3. 3.Division of Biomaterials and Tissue EngineeringUCL Eastman Dental InstituteLondonUK

Personalised recommendations