Journal of Materials Science

, Volume 46, Issue 24, pp 7923–7930 | Cite as

Effects of supercooling and organic solvent on the formation of a silk sponge with porous 3-D structure, and its dynamical and structural characterization using solid-state NMR

  • Tsunenori Kameda
  • Tomoko Hashimoto
  • Yasushi TamadaEmail author


A widely accepted feature of the silk generated by silkworms is its impressive mechanical properties. Aside from its direct use as silk fibers, silk protein can also be dissolved and converted into hydrogels [1], films [2], fibers [3], beads [4], electrospun nonwoven fibroin [5], and sponges, leading to great versatility in use. Silk sponge is the generic name for the porous 3-D structure made of silk fibroin. Many methods have been explored for the formation of a silk sponge. Freeze-drying of fibroin hydrogels [1] or fibroin solution in the presence of an appropriate cross-linker [6] yields silk sponges. Salt leaching and gas foaming are also used to form silk sponges [7, 8]. Silk sponges can also formed by repeated freezing and thawing [9].

One of the authors developed an original method to prepare silk sponges, which is shown in Fig.  1 [ 10]. The process involved freezing and thawing an aqueous solution of fibroin in the presence of a small amount of an organic solvent....


Nuclear Magnetic Resonance Sponge Silk Fibroin Random Coil Random Coil Structure 



This study was supported in part by the Agri-Health Translational Research Project and Research and development project for application in promoting a new policy for Agriculture Forestry and Fisheries.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Tsunenori Kameda
    • 1
  • Tomoko Hashimoto
    • 1
  • Yasushi Tamada
    • 1
    Email author
  1. 1.National Institute of Agrobiological SciencesTsukubaJapan

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