Elaboration, characterization and study of a novel affinity membrane made from electrospun hybrid chitosan/nylon-6 nanofibers for papain purification
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Electrospun hybrid chitosan/nylon-6 nanofibrous mats with fiber diameters in the range of 80–310 nm were successfully fabricated using an electrospinning method. Nanofibrous membranes were prepared by nucleophilic reaction of the chitosan’s hydroxyl and amidocyanogen with the triazinyl chloride of Cibacron Blue F3GA (CB) ligand. This system was used to study the purification of papain. Physical and chemical properties of the affinity membrane were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), contact angle (CA) and element analysis (EA). The equilibrium adsorption capacity (from Langmuir isotherm data) for papain was 93.46 mg/g affinity membrane. Fifteen layers of the composite affinity membrane were packed into a spin column to separate papain from raw material. Significant amount of the adsorbed papain (about 90.4%) was eluted by 1.0 M NaSCN at pH 9.0, and 4.8-fold purification was achieved in a single step. Experiments on regeneration and dynamic adsorption were also performed. It is shown that this system has the potential to be developed for the industrial purification of the papain.
This work was supported by the National Natural Science Foundation of China (50773009). This work was also supported in part by Grant 08JC1400600 of Science and Technology Commission of Shanghai Municipality and UK-CHINA Joint Laboratory for Therapeutic Textiles based in Donghua University and Biomedical Textile Materials “111 Project”, Ministry of Education of P.R. China (No. B07024).
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