Critical fluid shear stress analysis for cell–polymer adhesion
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A simple methodology to assess cell adhesion on materials was developed. We demonstrated that the cell adhesion strength could be quantified. Using this method, we were able to compare the NIH/3T3 Swiss mouse fibroblasts adhesion strength to poly(methyl methacrylate) and polycarbonate. A controlled fluid shear stress was applied to cells using a parallel plate rotational system. Cells detached from the surface in the radial direction. Results showed that there was a critical radius where the shear stress experienced by the cells equaled the cell adhesion strength. The cells outside this radius were removed while those inside maintained initial confluency. The quantitative evaluation of cell adhesion is beneficial for development of biomaterials.
KeywordsShear Stress PMMA Adhesion Strength Maximum Shear Stress Critical Radius
This research was in part sponsored by the NSF (0535578), the NSF–Louis Stokes Alliance for Minority Participation, Bridge-to-the-Doctorate Fellowship, the Texas A&M University, and the Texas Engineering Experiment Station (TEES). SEM analysis by Song Du, cell culture experiments assisted by Daniel Munoz Pinto, and support on TA Instruments by Sean Kohl are greatly appreciated.
- 7.Curtis ASG, Lackie JM (1991) Measuring cell adhesion. Wiley, New YorkGoogle Scholar
- 15.Channavajjala LS, Eidsath A, Saxinger WC (1997) J Cell Sci 110:249Google Scholar
- 16.Tateishi T, Ushida T (1995) Cell adhesion strength to bioceramics and morphology, Southern biomedical engineering conference, Proceedings, 14th Southern biomedical engineering conference, April 7–9, 1995. Louisiana State University Medical School, IEEE, Biomedical Research Foundation of Northwest Louisiana, pp 278–281Google Scholar
- 29.Alonso S, Lau J, Jaber BL (2008) Biocompatible hemodialysis membranes for acute renal failure (review). In: Cochrane database of systematic reviews. Wiley, New York, pp 1–38Google Scholar
- 32.Papadaki M, McIntire LV (1999) Tiss Eng Methods Protocols 18:577Google Scholar
- 34.Pollard TD, Earnshaw WC (2002) Cell biology. Sounders, Philadelphia, PAGoogle Scholar
- 35.Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (1994) Molecular biology of the cell. Garland Publishing, New YorkGoogle Scholar
- 41.Underwood PA, Steele JG, Dalton BA (1993) J Cell Sci 104:793Google Scholar