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Journal of Materials Science

, Volume 45, Issue 2, pp 475–482 | Cite as

Electrochemical and morphological studies of an electroactive material derived from 3-hydroxyphenylacetic acid: a new matrix for oligonucleotide hybridization

  • Ronalt M. L. Oliveira
  • Sabrina N. Vieira
  • Heden C. Alves
  • Erick G. França
  • Diego L. Franco
  • Lucas F. Ferreira
  • Ana G. Brito-Madurro
  • João M. MadurroEmail author
Article

Abstract

This paper describes the formation of polymeric films derived from 3-hydroxyphenylacetic acid electropolymerized onto graphite electrodes through cyclic voltammetry. We observed the formation of an electroactive material over the electrode surface. The modified electrode showed significant blocking behavior to electron transfer reaction of the pair redox ferricyanide/ferrocyanide, indicating repulsion electrostatic with the negatively charged carboxylate groups of the polymer. The quasi-reversible behavior to Ru(NH3)6Cl2 suggests electrostatic attraction, facilitating the charge transfer. The modified electrode was studied through electrochemical quartz crystal microbalance, electrochemical impedance spectroscopy, and atomic force microscopy. These analyses indicate modification of the graphite electrode. Surface analysis by AFM showed that the morphology of the modified electrode surface presents globular form, randomly distributed, and formed by lower globules with diameter near 100 nm. Immobilization and hybridization of oligonucleotide onto the modified electrode were successfully carried out by using both direct electrochemical oxidation of nitrogenated bases and the redox electroactive indicator methylene blue.

Keywords

Methylene Blue Electrochemical Impedance Spectroscopy Graphite Electrode Graphite Surface Electrochemical Quartz Crystal Microbalance 

Notes

Acknowledgements

The authors are grateful for financial support from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG). Also, we would like to thank teacher Abílio Borghi for the revision of the English manuscript.

References

  1. 1.
    Griese S, Kampouris DK, Kadara RO, Banks CE (2008) Electroanalysis 20:1507CrossRefGoogle Scholar
  2. 2.
    Malinauskas A (1999) Synth Met 107:75CrossRefGoogle Scholar
  3. 3.
    Castro CM, Vieira SN, Gonçalves RA, Madurro AGB, Madurro JM (2008) J Mater Sci 43:475. doi: https://doi.org/10.1007/s10853-007-1880-7 CrossRefGoogle Scholar
  4. 4.
    Lojou E, Bianco P (2004) Electroanalysis 16:1113CrossRefGoogle Scholar
  5. 5.
    Ahujaa T, Mira IA, Kumara D, Rajesh DK (2007) Biomaterials 28:791CrossRefGoogle Scholar
  6. 6.
    Gerard M, Chaubey A, Malhotra BD (2002) Biosens Bioelectron 17:345CrossRefGoogle Scholar
  7. 7.
    Silva TAR, Ferreira LF, Souza LM, Goulart LR, Madurro JM, Brito-Madurro AG (2009) Mater Sci Eng C 29:539CrossRefGoogle Scholar
  8. 8.
    Xie Y, Jiang F, Xu J, Zeng L, Dong B, Lu B, Shang X (2009) Eur Polym J 45:418CrossRefGoogle Scholar
  9. 9.
    Franco DL, Afonso AS, Vieira SN, Ferreira LF, Gonçalves RA, Madurro AGB, Madurro JM (2008) Mater Chem Phys 107:404CrossRefGoogle Scholar
  10. 10.
    Vieira SN, Ferreira LF, Franco DL, Afonso AS, Gonçalves RA, Madurro AGB, Madurro JM (2006) Macromol Symp 245–246:236CrossRefGoogle Scholar
  11. 11.
    Franco DL, Afonso AS, Ferreira LF, Gonçalves RA, Boodts JFC, Brito-Madurro AG, Madurro JM (2008) Polym Eng Sci 48:2043CrossRefGoogle Scholar
  12. 12.
    Lofrano RCZ, Madurro JM, Abrantes LM, Romero JR (2004) J Mol Catal A Chem 218:73CrossRefGoogle Scholar
  13. 13.
    Lofrano RCZ, Madurro JM, Romero JR (2000) J Mol Catal A Chem 153:237CrossRefGoogle Scholar
  14. 14.
    Ferreira LF, Boodts JFC, Madurro AGB, Madurro JM (2008) Polit Int 57:644Google Scholar
  15. 15.
    Zhang Y, Zhang K, Ma H (2009) Anal Biochem 387:13CrossRefGoogle Scholar
  16. 16.
    Madurro AGB, Ferreira LF, Vieira SN, Goulart LR, Madurro JM (2007) J Mater Sci 42:3238. doi: https://doi.org/10.1007/s10853-006-0235-0 CrossRefGoogle Scholar
  17. 17.
    Hauschild A, Karki K, Cowie BCC, Rohlfing M, Tautz FS, Sokolowski M (2005) Phys Rev Lett 94:036106CrossRefGoogle Scholar
  18. 18.
    Cao Xiao-Yu, Zhang Wen-Bin, Wang Jin-Liang, Xing-Hua Zhou H, Lu PeiJ (2003) J Am Chem Soc 125:12430CrossRefGoogle Scholar
  19. 19.
    Palecek E, Fojta M (2001) Anal Chem 73:74AGoogle Scholar
  20. 20.
    Ouajai WP, Pigram PJ, Jones R, Sirivat A (2008) Sensor Actuat B-Chem 135:366CrossRefGoogle Scholar
  21. 21.
    Pingarrón JM, Sedeño PY, Cortés AG (2008) Electrochim Acta 53:5848CrossRefGoogle Scholar
  22. 22.
    Li N, Zhao H, Yuan R, Peng K, Chai Y (2008) Electrochim Acta 54:235CrossRefGoogle Scholar
  23. 23.
    Silva FB, Vieira SN, Goulart Filho LR, Boodts JFC, Brito-Madurro AG, Madurro JM (2008) Int J Mol Sci 9:1188CrossRefGoogle Scholar
  24. 24.
    Teles FRR, Fonseca LP (2008) Talanta 77:606CrossRefGoogle Scholar
  25. 25.
    Silva TAR, Ferreira LF, Boodts JFC, Eiras SP, Madurro JM, Brito-Madurro AG (2008) Polym Eng Sci 48:1963CrossRefGoogle Scholar
  26. 26.
    Erdem A, Kerman K, Meric B, Akarca US, Ozsoz M (2000) Anal Chim Acta 422:139CrossRefGoogle Scholar
  27. 27.
    Pellissier M, Barrière F, Downard AJ, Leech D (2008) Electrochem Commun 10:835CrossRefGoogle Scholar
  28. 28.
    Sauerbrey GZ (1959) Phys 155:206CrossRefGoogle Scholar
  29. 29.
    Daniels JS, Pourmanda N (2007) Electroanalysis 19:1239CrossRefGoogle Scholar
  30. 30.
    Oliveira-Brett AM, Piedade JA, Silva LA, Diculescu VC (2004) Anal Biochem 332:321CrossRefGoogle Scholar
  31. 31.
    Arora K, Prabhakar N, Chand S, Malhotra BD (2007) Anal Chem 79:6152CrossRefGoogle Scholar
  32. 32.
    Erdem A, Kerman K, Meric B, Akarca US, Ozsoz M (2002) Turk J Chem 26:851Google Scholar
  33. 33.
    Erdem A, Kerman K, Meric B, Ozsoz M (2001) Electroanalysis 13:219CrossRefGoogle Scholar
  34. 34.
    Prabhakar N, Singh H, Malhotra BD (2008) Electrochem Commun 10:821CrossRefGoogle Scholar
  35. 35.
    Kara P, Kerman K, Ozkan D, Meric B, Erdem A, Ozkan Z, Ozsoz M (2002) Electrochem Commun 4:705CrossRefGoogle Scholar
  36. 36.
    Ye KY, Zhao JH, Yan F, Zhu YL, Ju HX (2003) Biosens Bioelectron 18:1501CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Ronalt M. L. Oliveira
    • 1
  • Sabrina N. Vieira
    • 1
  • Heden C. Alves
    • 1
  • Erick G. França
    • 1
  • Diego L. Franco
    • 1
  • Lucas F. Ferreira
    • 1
  • Ana G. Brito-Madurro
    • 2
  • João M. Madurro
    • 1
    Email author
  1. 1.Institute of Chemistry, Federal University of UberlândiaUberlândiaBrazil
  2. 2.Institute of Genetics and Biochemistry, Federal University of UberlândiaUberlândiaBrazil

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