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Journal of Materials Science

, Volume 44, Issue 4, pp 954–961 | Cite as

Poly (pyrrole-co-N-methyl pyrrole) for application to cholesterol sensor

  • K. Singh
  • T. Basu
  • Pratima R. Solanki
  • Bansi Dhar Malhotra
Article

Abstract

Cholesterol oxidase (ChOx) has been electrochemically entrapped onto p-toluene sulphonate (PTS) doped poly (pyrrole-co-N-methyl pyrrole) (1:1) on indium-tin-oxide (ITO) glass plates. This ChOx-copolymer-PTS/ITO bioelectrode has been characterized using cyclic voltammetry (CV), Fourier transform infrared (FTIR) spectroscopy, UV-visible spectroscopy, scanning electron microscopy (SEM) techniques. The cholesterol bioelectrode shows response time of 19 s, linearity from 50 to 400 mg/dL as a function of cholesterol concentration. It exhibits optimum pH range between 6.5 and 7.5, shelf-life of up to 6 weeks at 4 °C and shows almost undisturbed response in presence of interferents like ascorbic acid, uric acid and glucose.

Keywords

Cholesterol Pyrrole Cholesterol Oxidase Enzyme Electrode Cyclic Voltammetric Study 

Notes

Acknowledgements

Tinku Basu is thankful to Dr. Ashok K.Chauhan, Founder President of Amity University, U.P., India, for his constant encouragement and motivation to perform the innovative work in the development of Science and Technology. We thank Director, National Physical Laboratory, New Delhi, India, for the facilities. PRS is grateful to the Council of Scientific Industrial Research (CSIR), India, for the award of the Senior Research Associate (SRA).

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • K. Singh
    • 1
  • T. Basu
    • 2
  • Pratima R. Solanki
    • 3
  • Bansi Dhar Malhotra
    • 3
  1. 1.Amity Institute of Engineering and TechnologyAmity UniversityNoidaIndia
  2. 2.Amity Institute of Nano TechnologyAmity UniversityNoidaIndia
  3. 3.Biomolecular Electronics and Conducting Polymer Research GroupNational Physical LaboratoryNew DelhiIndia

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