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Journal of Materials Science

, Volume 43, Issue 16, pp 5618–5625 | Cite as

Evaluation of hydroxyapatite microspheres made from a borate glass to separate protein mixtures

  • Xue HanEmail author
  • Ming Du
  • Yinfa Ma
  • Delbert E. Day
Article

Abstract

A hydroxyapatite (HA, Ca10(PO4)6(OH)2), transformed from a calcium-containing borate glass, has been investigated for its protein adsorption and chromatographic characteristics. Microspheres of the borate glass were transformed into HA by reacting them with a 0.25 M phosphate (K2HPO4) solution for 24 h at 37 °C (pH 9.0). The HA microspheres with a diameter of 45–90 μm were hand packed into a steel column (4.6 mm × 80 mm) and used to separate a binary protein mixture of bovine serum albumin (BSA) and lysozyme. HA microspheres, with a diameter <45 μm, were used for separating a protein mixture of BSA, myoglobin, and lysozyme. These microspheres had a diameter that was 20–30 times larger than commercial HA column packing spherical particles, 2–3 μm, but these microspheres had a six times larger surface area and a more uniform spherical shape. These advantages compensated for their larger size and the separation results were comparable to those commercially available HA columns in the separation of the proteins studied. These unique HA microspheres, made from microspheres of a borate glass, are considered to be useful as packing materials for protein separation in chromatography.

Keywords

High Performance Liquid Chromatography Lysozyme Phosphate Solution Protein Mixture Glass Particle 

Notes

Acknowledgement

The financial support from the UMR Graduate Center for Materials Research is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of Materials Science and EngineeringJohns Hopkins UniversityBaltimoreUSA
  2. 2.Department of ChemistryMissouri University of Science and TechnologyRollaUSA
  3. 3.Graduate Center for Materials Research and Ceramic Engineering DepartmentMissouri University of Science and TechnologyRollaUSA

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