Journal of Materials Science

, Volume 42, Issue 17, pp 7566–7574 | Cite as

A simple synthesis of amine-derivatised superparamagnetic iron oxide nanoparticles for bioapplications

  • Sasmita Mohapatra
  • Nabakumar Pramanik
  • Samrat Mukherjee
  • Sudip K. Ghosh
  • Panchanan Pramanik


Adsorption of 3-aminopropyltriethoxysilane (APTS) on magnetite nanoparticles during its formation has been investigated to optimise the preparation of stable aqueous dispersion of amine derivatised magnetite nanoparticles. APTS adsorbs chemically on the surface of magnetite particle modifying its surface which is evident from thermal and C, H, N analysis. The variation of particle size has been observed with change of APTS concentration. X-ray diffractogram shows the formation of pure inverse spinel phase magnetite with average crystallite size 7 nm when equimolar (Fe3O4: APTS = 1:1) quantity of APTS was used during its synthesis. The presence of free surface –NH2 groups and Fe–O–Si bonds was observed by FTIR. Raman spectrum further confirms the presence of surface –NH2 groups. Transmission electron microscopy shows formation of particles of average size between 7 nm and 12 nm. The effective hydrodynamic diameter of the APTS coated particle agglomerates is 45.8 nm in stable aqueous colloidal dispersion, which is evident from photon correlation spectroscopy. VSM measurements at room temperature of both silanised and unsilanised magnetite shows their superparamagnetic nature with saturation magnetisation 41 e.m.u/g and 56 e.m.u/g, respectively. Avidin has been immobilised on the surface through glutaraldehyde, which demonstrates the possibility of the synthesised material to be used in protein immobilisation to form bioactive magnetic particles.



Authors are thankful to CSIR New Delhi for providing financial support for this work. Dr D. Das, IUC-DAE Consortium for Scientific Research, Kolkata, Dr M. K. Panigrahi, IIT Kharagpur and Dr D. Bahadur, IIT Bombay, Powai are gratefully acknowledged for Mössbauer, Raman and VSM studies, respectively.


  1. 1.
    Chaterjee J, Haik Y, Chen CJ (2001) J Magn Magn Mater 225:21CrossRefGoogle Scholar
  2. 2.
    Bilková Z, Slováková M, Horák D, Lenfeld J (2002) J Chromatogr B 770:177CrossRefGoogle Scholar
  3. 3.
    Pourfarzaneh M, Camel RS, London J, Dawes CC (1982) Method Biochem Anal 28:267Google Scholar
  4. 4.
    Homes E, Korsnes L (1990) Genet Anal Technol Appl 7:145Google Scholar
  5. 5.
    Sonti SV, Bose A (1991) J Colloid Inter Sci 170:575CrossRefGoogle Scholar
  6. 6.
    Cupta PK, Hung CT (1989) Life Sci 44:175CrossRefGoogle Scholar
  7. 7.
    Coradin T, Lopez PJ (2003) Chembiochem 4:251CrossRefGoogle Scholar
  8. 8.
    Whitehead RA, Chagnon MS, Groman EU, Josephson L (1985) USP 4554,088Google Scholar
  9. 9.
    Ma M, Zhang Y, Yu W (2003) Colloids Surfaces A: Physicochem Eng Aspects 212(2–3):219Google Scholar
  10. 10.
    Yamaura M, Sampaio LC, Macêdo MA, Nakamura M, Toma HE (2004) J Magn Magn Mater 279(2–3):210CrossRefGoogle Scholar
  11. 11.
    Shen X-C, Fang X-Z, Zhou Y-H, Liang H (2004) Chem Lett 33(11):1468–1469CrossRefGoogle Scholar
  12. 12.
    Kobayashi H, Matsunaga T (1991) J Colloid Interface Sci 141:505CrossRefGoogle Scholar
  13. 13.
    Massart R, Cabuil V (1987) J Chem Phys 84(7–8):967Google Scholar
  14. 14.
    Bee A, Massart R (1990) J Magn Magn Mater 122:1Google Scholar
  15. 15.
    Cornell RM, Schwertmann U (1996) The iron oxides: structure, properties, reactions, occurrence and uses. Wiley-VCH, Weinheim, p 207Google Scholar
  16. 16.
    Brinker CJ, Schere GW (1990) Sol–gel science: the physics and chemistry of sol-gel processing. Academic Press, IncGoogle Scholar
  17. 17.
    Cornell RM, Schwertmann U (1996) The iron oxides: structure, properties, reactions, occurrence and uses. Wiley-VCH, Weinheim, p 135Google Scholar
  18. 18.
    De Faria LA, Venâncio Silva S, De Oliveria MT (1997) J Raman Spectroscopy 28:873CrossRefGoogle Scholar
  19. 19.
    Hartridge A, Bhattacharya AK, Sengupta M, Majumdar CK, Das D, Chintalapudi SN (1997) J Magn Magn Mater 176:L89CrossRefGoogle Scholar
  20. 20.
    Rao ZM, Wu TH, Peng SY (1995) Acta Phys Chim Sin 11:395Google Scholar
  21. 21.
    Mckittrick MW, Jones CW (2003) Chem Mater 15:1132CrossRefGoogle Scholar
  22. 22.
    Bruce IJ, Sen T (2005) Langmuir 21:7029Google Scholar
  23. 23.
    Ambastha RD, Wattal PK, Singh S, Bahadur D (2003) J Magn Magn Mater 267:335CrossRefGoogle Scholar
  24. 24.
    Bruke NAD, Stöver HDH, Dawson FP (2002) Chem Mater 14:4752CrossRefGoogle Scholar
  25. 25.
    Cullity BD (1972) Introduction to magnetic materials. Addison-Wesley Publishing Company, p 190Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Sasmita Mohapatra
    • 1
  • Nabakumar Pramanik
    • 1
  • Samrat Mukherjee
    • 3
  • Sudip K. Ghosh
    • 2
  • Panchanan Pramanik
    • 1
  1. 1.Department of ChemistryIndian Institute of TechnologyKharagpurIndia
  2. 2.Department of BiotechnologyIndian Institute of TechnologyKharagpurIndia
  3. 3.UGC-DAE Consortium for Scientific ResearchKolkataIndia

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