Interactions between cyclodextrins and fluorescent T-2 and HT-2 toxin derivatives: a physico-chemical study

  • Andrea Ventrella
  • Raffaella Verrone
  • Francesco Longobardi
  • Angela Agostiano
  • Vincenzo Lippolis
  • Michelangelo Pascale
  • Chris M. Maragos
  • Michael Appell
  • Lucia Catucci
Original Article

Abstract

T-2 and HT-2 toxins are mycotoxins produced by several Fusarium species that are commonly found in various cereal grains, including oats, barley, wheat and maize. Intake estimates indicate that the presence of these mycotoxins in the diet can be of concern for public health. In this work, the inclusion processes occurring between fluorescent anthracene-derivatives of T-2 and HT-2 toxins and different cyclodextrin (CD) molecules were investigated in aqueous solutions by means of UV–Vis absorption, fluorescence emission and dynamic light scattering. Binding constant values and chemico-physical parameters were calculated. It was found that β-CDs give stronger inclusion reactions with both T-2 and HT-2 derivatives, as stated by important emission intensity increments. Such interactions were found to be fundamentally enthalpy-driven. Among β-CDs, the effect of the methylation at hydroxyl groups was tested: as a result, the di-methyl form of β-CD was found to induce the best fluorescence intensity enhancements.

Keywords

T-2 toxin HT-2 toxin Cyclodextrin Fluorescence Inclusion complex 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Andrea Ventrella
    • 1
  • Raffaella Verrone
    • 1
  • Francesco Longobardi
    • 1
  • Angela Agostiano
    • 1
    • 2
    • 3
  • Vincenzo Lippolis
    • 4
  • Michelangelo Pascale
    • 4
  • Chris M. Maragos
    • 5
  • Michael Appell
    • 5
  • Lucia Catucci
    • 1
    • 2
  1. 1.Dipartimento di ChimicaUniversità degli Studi di Bari “Aldo Moro”BariItaly
  2. 2.IPCF-CNR, sez. BariBariItaly
  3. 3.INSTMFlorenceItaly
  4. 4.Institute of Sciences of Food Production (ISPA)CNR-National Research Council of ItalyBariItaly
  5. 5.Bacterial Foodborne Pathogens and Mycology Research UnitUSDA-ARS-NCAURPeoriaUSA

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