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Spectroscopic investigation of Rose Bengal/cyclodextrin interactions in aqueous solution: the case of the hydroxypropyl-cyclodextrins

  • P. FiniEmail author
  • L. Catucci
  • M. Castagnolo
  • P. Cosma
  • V. Pluchinotta
  • A. Agostiano
Original Article

Abstract

The interaction of Rose Bengal (RB) with hydroxypropyl-α-cyclodextrin (HP-α-CD), hydroxypropyl-β-cyclodextrin (HP-β-CD) and hydroxypropyl-γ-cyclodextrin (HP-γ-CD) has been studied in water and in acetate buffer at pH 4.5 by UV–Vis absorption, fluorescence spectroscopy and Induced Circular Dichroism at 298 K. Evidence of the complex formation between the RB and all HP-CDs have been obtained both in water and in buffer. Binding constants and stoichiometry of RB/HP-CD complexes in water have been determined by applying the modified Benesi-Hildebrand equation to the fluorescence measurements.

Keywords

Rose Bengal Aqueous solutions Hydroxypropyl-Cyclodextrins Inclusion complexes pH 

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • P. Fini
    • 1
    Email author
  • L. Catucci
    • 1
    • 2
  • M. Castagnolo
    • 2
  • P. Cosma
    • 1
    • 2
  • V. Pluchinotta
    • 2
  • A. Agostiano
    • 1
    • 2
  1. 1.Istituto per i Processi Chimico Fisici (IPCF) CNRBariItaly
  2. 2.Dipartimento di ChimicaUniversita’ di BariBariItaly

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