In vitro study of a HPγ-cyclodextrin grafted PET vascular prosthesis for application as anti-infectious drug delivery system

  • N. Blanchemain
  • T. Laurent
  • S. Haulon
  • M. Traisnel
  • C. Neut
  • J. Kirkpatrick
  • M. Morcellet
  • H. F. Hildebrand
  • Bernard Martel
Original Article

Abstract

Hydroxypropyl-γ-cyclodextrin (HPγ-CD) was grafted onto woven polyester (PET) vascular prosthesis by using citric acid (CTR) as crosslinking agent. A polyCTR-HPγ CD polymer was physically fixed onto the PET fibers. An optimal compromise between fixation temperature and fixation time was found and a grafting rate of 6.7% was obtained. The study of the inclusion of ciprofloxacin (CFX) and HPγ-CD was evidenced by using spectrophotometry. Sorption tests also showed that modified prosthesis could adsorb 5 times more CFX than the control. Biological tests revealed proliferation rates of human pulmonary micro-vascular endothelial cells (HPMEC) of 73 and 48% on virgin and modified prostheses respectively. We demonstrated that this was rather due to the increase of surface roughness of the fibers after their modification than to a toxic effect the polyCTR-HPγCD polymer coating. Prostheses samples modified with HPγCD and impregnated with CFX stayed up to 24 h in blood plasma. At various moments some aliquots were withdrawn from the medium and a positive antibacterial activity against Staphylococcus epidermidis was observed within the 24 h period for the grafted sample, whilst that of the virgin one had disappeared within 4 h. So, cyclodextrin coating of vascular prostheses may be suitable for the controlled release of CFX, and thus should help to the prevention of post surgery complications.

Key words

Polyester vascular prosthesis Cyclodextrins Grafting Biocompatibility Endothelial cells Microbiology Ciprofloxacin Staphylococcus epidermidis 

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • N. Blanchemain
    • 1
    • 2
  • T. Laurent
    • 1
  • S. Haulon
    • 2
  • M. Traisnel
    • 3
  • C. Neut
    • 4
  • J. Kirkpatrick
    • 5
  • M. Morcellet
    • 1
  • H. F. Hildebrand
    • 2
  • Bernard Martel
    • 1
  1. 1.Laboratoire de Chimie Organique et MacromoléculaireUMR-CNRS 8009, USTLVilleneuve D’AscqFrance
  2. 2.Faculté de MédecineGroupe de Recherche sur les Biomatériaux, EA 1049LilleFrance
  3. 3.Laboratoire de Procédés et d’Elaboration de Revêtements FonctionnelsUPRES EA 1040, ENSCLVilleneuve D’AscqFrance
  4. 4.Laboratoire de Bactériologie Clinique, Faculté de PharmacieINSERM U 795LilleFrance
  5. 5.Institute of PathologyJohannes Gutenberg UniversityMainzGermany

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