Guided tissue regeneration membranes with controlled delivery properties of chlorhexidine by their functionalization with cyclodextrins

  • S. Leprêtre
  • F. Boschin
  • N. Tabary
  • M. Bria
  • B. Martel
  • N. Blanchemain
  • H. F. Hildebrand
  • M. Morcellet
  • E. Delcourt-Debruyne
Original Article

Abstract

In parodontology, guided tissue regeneration (GTR) is a new technique to cure periodontal lesions. Where the association of the GTR with an antimicrobial agent does not yield optimal results, we used the properties of cyclodextrins (CDs) to improve the membrane used in RTG to control the release and to increase the quantity of antimicrobial agent stocked on the membrane. We successed in fixing 14%-wt of cyclodextrin polymer on polyvinylidene difluoride (PVDF) membranes thank to citric acid (CTR) as crosslinking agent. We studied the complexation of chlorhexidine diacetate (CHX), the antiseptic agent used in this study, with CDs in UV-spectrophotometry and ROESY NMR. We observed complexation of CHX by β, γ, hydroxypropylated (HP) βCD. We studied the biological properties of the cyclodextrin polymer onto (PVDF) membranes and observed that the CDs-polymer is not harmful for the cells. Moreover it stimulates their growth with native CD. A kinetic of release of the CHX was performed. Raw membranes released all CHX stocked in few hours, whereas grafted membranes released more than tenfold this quantity during 60–80 days.

Keywords

Biomaterials Cyclodextrins Drug delivery system Periodontal Polycarboxylic acids Tissue regeneration 

References

  1. 1.
    Nyman, S., Gottlow, J., Karring, T., Lindhe, J.: The regenerative potential of the periodontal ligament. J. Clin. Periodontal. 9, 257 (1982)CrossRefGoogle Scholar
  2. 2.
    Veron, C., Chanavaz, M., et al.: Biomatériaux et biocompatibilité. Panorama des matériaux actuels pour apport osseux en chirurgie maxillo-faciale et implantologie orale. Rev. Stomatol. Chir. Maxillofac. 96, 274 (1995)Google Scholar
  3. 3.
    De Sanctins, M., Zuchelli, G., Clauser, C.: Bacterial colonization of barrier material and periodontal regeneration. J. Clin. Period. 23, 1039 (1996)CrossRefGoogle Scholar
  4. 4.
    Zuchelli, G., Sforza, N.M., et al.: Topical and systematical antimicrobial therapy in GTR. J. Period. 70, 239 (1999)CrossRefGoogle Scholar
  5. 5.
    Alpar, B., Leyhausen, G., Gunay, H., Geursten, W.: Compatibility of non resorbable guided tissue regeneration membranes in cultures of primary human periodontal ligament fibroblasts and human osteoblastlike cells. Clin. Oral Invest. 4, 219 (2000)CrossRefGoogle Scholar
  6. 6.
    Yoshinari, N., Tohya, T., et al.: Effects of a repeated local minocycline administration on periodontal healing following guided tissue regeneration. J. Period. 72, 284 (2001)CrossRefGoogle Scholar
  7. 7.
    Zarkesh, N., Nowzari, H., Morrison, J.L., Slots, J.: Tetracycline-coated polytetrafluoroethylene barrier membranes in the treatment of intraosseous periodontal lesions. J. Period. 70, 1009 (1999)Google Scholar
  8. 8.
    Markman, C., Fracalanzza, S.E.L., Novaes, A.B., Jr.: Slow release of Tetracycline hydrochloride from a cellulose membrane used in guided tissue regeneration. J. Period. 66(11), 978 (1995)Google Scholar
  9. 9.
    Boschin, F., Jimenez, M., Meziane, R., Delcourt-Deburyne, E., Hildebrand, H.F., Morcellet, M., Martel, B.: Interest of cyclodextrins in periodontal guided tissue regeneration: release of chlorhexidine by PVDF membranes. In: Proceedings of the XIIth International Cyclodextrin Symposium, pp. 647–650. Montpellier, 16–19 May 2004Google Scholar
  10. 10.
    Martel, B., Morcellet, M., Weltrowski, M.: PCT/FR00/00377 (2000)-US 09/913,475 (2001)Google Scholar
  11. 11.
    Martel, B., Morcellet, M., Weltrowski, M.: PCT/FR00/00378 (2000) US 09 913,448 (2001)Google Scholar
  12. 12.
    Martel, B., Blanchemain, N., Morcellet, M., Hildebrand, H.F., Haulon, S., Bouchin, F., Delcourt-Debruyne, E.: Patent application FR2 877 846, PCT/FR2005/002829 WO 2006/051227, (2006)Google Scholar
  13. 13.
    Ferreira Franco, C., Pataro, A.L., et al.: In vitro effects of a chlorhexidine controlled delivery system. Artfi. Organs 27(5), 486 (2003)CrossRefGoogle Scholar
  14. 14.
    Chen, Y.T., Hung, S.L., Lin, L.W., Chi, L.Y., Ling, L.J.: Attachment of periodontal ligament cells to chlorhexidine-loaded guided tissue regeneration membranes. J. Period. 74(11), 1652 (2003)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • S. Leprêtre
    • 1
  • F. Boschin
    • 2
    • 3
  • N. Tabary
    • 1
  • M. Bria
    • 1
  • B. Martel
    • 1
  • N. Blanchemain
    • 2
  • H. F. Hildebrand
    • 2
  • M. Morcellet
    • 1
  • E. Delcourt-Debruyne
    • 3
  1. 1.Laboratoire de Chimie Organique et MacromoléculaireCNRS-UMR 8009, USTLVilleneuve d’AscqFrance
  2. 2.Groupe de Recherche sur les Biomatériaux, Faculté de MédecineLaboratoire de Biophysique UPRES EA 1049LilleFrance
  3. 3.Laboratoire de biologie du ParodonteFaculté de Chirurgie DentaireLilleFrance

Personalised recommendations