Formation of Cyclodextrin Inclusion Complexes with Doxycyclin-Hyclate: NMR Investigation of Their Characterisation and Stability

  • Youssef Bakkour
  • Gaston Vermeersch
  • Michel Morcellet
  • FranÇois Boschin
  • Bernard Martel
  • Nathalie Azaroual
Article

Abstract

The solubility of cyclodextrin (CD) can be notably improved when it is included in a polymeric structure. CD was reacted with citric acid, yielding a water-soluble polymer whose inclusion properties towards doxycyclin-hyclate (DOX) as guest molecule were investigated by NMR. The new DOSY (Diffusion Ordered SpectroscopY) method, based on diffusion coefficient measurements is convenient study complexes made of large molecules and it was applied to determine the association constants between DOX and parent β and γ-CD and their polymerised forms. The association constant obtained by DOSY was compared with that determined more classically by the chemical shift variation measurement using Scott's plot.

Key words

association constant DOSY NMR doxycyclin-hyclate 1H NMR inclusion complex polymer cyclodextrin ROESY 2D 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Youssef Bakkour
    • 1
  • Gaston Vermeersch
    • 1
  • Michel Morcellet
    • 2
  • FranÇois Boschin
    • 2
  • Bernard Martel
    • 2
  • Nathalie Azaroual
    • 1
  1. 1.Laboratoire de PhysiqueUMR CNRS 8009, Faculté des Sciences Pharmaceutiques et BiologiquesLilleFrance
  2. 2.Laboratoire de Chimie Organique et MacromoléculaireUMR CNRS 8009, Université des Sciences et Technologie de Lille 1Villeneuve d' AscqFrance

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