Effects of fragmentation and anthropic pressure on the genetic structure of Canthon (Peltecanthon) staigi (Coleoptera: Scarabaeidae) populations in the Atlantic Forest domain
- 224 Downloads
Canthon (Peltecanthon) staigi is an endemic dung beetle from the east coast of South America (Brazil, Paraguay and Argentina). It is a stenotopic species abundant in different habitats of the Atlantic Forest. In this study, we analyzed the influence of fragmentation and environmental changes on the population genetic structure of C. (P.) staigi using inter-simple sequence repeat polymorphisms, and the consequences for management and conservation. The expected heterozygosity (HE) ranged from 0.16 (restinga, TAM population) in the smallest and most disturbed forest patch to 0.24 (“brejo de altitude”, PES population) in the largest and most conserved area. Based on the low coefficient of genetic population differentiation (F ST = 0.07) and large molecular variance within populations, we suggest historical gene flow among C. (P.) staigi populations. Additionally, the model ƒ = 0, which corresponds to a lack of or very low inbreeding rate, is another factor that indicates gene flow among populations. Two genetic traits (K = 2) were related with anthropic disturbance, suggesting that the six studied populations are genetically divided into two separate units, one related to preserved and another to disturbed areas. Based on these data, the recent habitat fragmentation may not have led to genetic differentiation, but rather the environmental modifications caused by anthropic pressure seem to be strongly related to the loss of genetic diversity in the species C. (P.) staigi.
KeywordsISSR Genetic diversity Scarabaeinae Dung beetles
We are grateful to MSc Cristiane Maria Queiroz da Costa and Prof. Dr. Fernando Silva for the collection and identification of specimens. This study was supported by a Grant from the Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE-IBPG-0831-2.02/11) through a Doctorate Scholarship to C.A.F.N., a Post-doc Researcher Fellowship to G.A.S.C. from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PNPD-20130558) and FACEPE-BCT-0063-2.02/14, and by funding from the CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) to R.C.M. (CNPQ- 305298/2014-3).
- Costa FC, Pessoa KKT, Liberal CN, Filgueiras BKC, Salomão RP, Iannuzzi L (2013) What is the importance of open habitat in a predominantly closed forest area to the dung beetle (Coleoptera, Scarabaeinae) assemblage? Rev Bras Entomol 57(3):329–334. doi: 10.1590/S0085-56262013000300012 CrossRefGoogle Scholar
- Frankham R, Ballou JD, Briscoe DA (2008) Fundamentos de genética da conservação. SBG, Ribeirão PretoGoogle Scholar
- Gaublomme E, Maebe K, Doninck KV, Dhuyvetter H, Li X, Desender K, Hendrickx F (2013) Loss of genetic diversity and increased genetic structuring in response to forest area reduction in a ground dwelling insect: a case study of the flightless carabid beetle Carabus problematicus (Coleoptera, Carabidae). Insect Conserv Divers 6:473–482CrossRefGoogle Scholar
- Halffter G, Martínez A (1967) Revisión monográfica de los Canthonina americanos (2ª parte). Rev Soc Mex Hist Nat 28:79–116Google Scholar
- Pereira FSCMS, Martines A (1956) Os gêneros de Canthonini Americanos (col. Scarabaeidae). Rev Bras Entomol 6:91–192Google Scholar
- Perovano TL, Lima RN (2003) Dinâmica de ocupação do solo na faixa de Reserva Ecológica de Vila Velha (ES). Nat Online 1:17–23Google Scholar
- Sokal RR, Rohlf FJ (1995) Biometry, 3rd edn. Freeman, New York, p 813–819Google Scholar
- Varshney RK, Chabane K, Hendre PS, Aggarwal RK, Graner A (2007) Comparative assessment of EST-SSR, EST-SNP and AFLP marker for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant Sci 173:638–649. doi: 10.1016/j.plantsci.2007.08.010 CrossRefGoogle Scholar