Advertisement

Radiofrequency identification tag system improves the efficiency of closed vitrification for cryopreservation and thawing of bovine ovarian tissues

  • T. Sato
  • Yodo Sugishita
  • Y. Suzuki
  • M. Kashiwagi
  • S. Furuyama
  • S. Nishimura
  • A. Uekawa
  • T. Koizumi
  • M. Awaji
  • T. Sawa
  • A. Tozawa
  • V. Komatsu
  • Nao SuzukiEmail author
Fertility Preservation
  • 20 Downloads

Abstract

Purpose

A radiofrequency identification (RFID) tag system was designed to streamline cryopreservation and thawing procedures. This study evaluated the usefulness of the RFID tag system for improving the efficiency of cryopreserving/thawing bovine ovarian tissue by the closed vitrification protocol.

Methods

Six participants carried out closed vitrification and thawing of bovine ovarian tissues procedures using either the conventional or the new RFID tag method, and the time required to perform each step of the respective methods was measured. After normality of data was confirmed by the Shapiro-Wilk test, the significance of differences was assessed by the unpaired t test.

Results

When closed vitrification was performed, the time required for each step showed a significant difference between the two methods (t(4) = 2.938, p = 0.042, d = 2.40), and the total cryopreservation time was 11 min shorter using the RFID tag system. When thawing was performed, the time required for each step also showed a significant difference between the two methods (t(4) = 2.797, p = 0.049, d = 2.28), and the total thawing time was 2 min shorter using the RFID tag system.

Conclusion

The RFID tag system tested in this study seems to be suitable for managing biological samples stored in liquid nitrogen. Adoption of an RFID tag system by fertility centers may not only improve the efficiency of cryopreserving/thawing reproductive tissues but could also reduce human error.

Keywords

Infertility treatment Cryopreservation Thawing Ovarian tissue closed vitrification Frozen samples RFID tag Storage system 

Notes

Acknowledgments

Dr. David Robert McQuire (Yamada Translation Bureau, Tokyo, Japan) assisted with manuscript editing.

Authors’ roles

Y.S., T.S., N.S., T.S., and H.K. participated in study conception and design; Y.S., T.S., and H.K. developed the device; T.S, Y.S., Y.S., M.K., S.F., and U.A. performed the study; T.K. performed the statistical analysis; M.A., T.S., and H.K. built the computer system; N.S. edited the English manuscript; N.S, A.T., and N.S. gave suggestions for this research.

Funding information

Grant-in-Aid for Scientific Research (B) from the Japan Society for the Promotion of Science to Nao Suzuki.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

References

  1. 1.
    Green DM, Liu W, Kutteh WH, Ke RW, Shelton KC, Sklar CA, et al. Cumulative alkylating agent exposure and semen parameters in adult survivors of childhood cancer: a report from the St Jude Lifetime Cohort Study. The Lancet Oncology. 2014;15(11):1215–23.CrossRefGoogle Scholar
  2. 2.
    Thomson AB, Campbell AJ, Irvine DC, Anderson RA, Kelnar CJ, Wallace WH. Semen quality and spermatozoal DNA integrity in survivors of childhood cancer: a case-control study. Lancet. 2002;360(9330):361–7.CrossRefGoogle Scholar
  3. 3.
    Aubier F, Flamant F, Brauner R, Caillaud JM, Chaussain JM, Lemerle J. Male gonadal function after chemotherapy for solid tumors in childhood. J Clin Oncol. 1989;7(3):304–9.CrossRefGoogle Scholar
  4. 4.
    Williams D, Crofton PM, Levitt G. Pediatr Blood Cancer. 2008;50(2):347–51.CrossRefGoogle Scholar
  5. 5.
    Lampe H, Horwich A, Norman A, Nicholls J, Dearnaley DP. Fertility after chemotherapy for testicular germ cell cancers. J Clin Oncol. 1997;15(1):239–45.CrossRefGoogle Scholar
  6. 6.
    Green DM, Kawashima T, Stovall M, Leisenring W, Sklar CA, Mertens AC, et al. Fertility of male survivors of childhood cancer: a report from the Childhood Cancer Survivor Study. J Clin Oncol. 2010;28(2):322–9.CrossRefGoogle Scholar
  7. 7.
    Howell SJ, Shalet SM. Testicular function following chemotherapy. Hum Reprod Update. 2001;7(4):363–9.CrossRefGoogle Scholar
  8. 8.
    Shalet SM, Tsatsoulis A, Whitehead E, Read G. Vulnerability of the human Leydig cell to radiation damage is dependent upon age. J Endocrinol. 1989;120(1):161–5.CrossRefGoogle Scholar
  9. 9.
    Fisch B, Abir R. Female fertility preservation: past, present and future. Reproduction. 2018;156(1):F11–27.CrossRefGoogle Scholar
  10. 10.
    Kristensen SG, Andersen CY. Cryopreservation of ovarian tissue: opportunities beyond fertility preservation and a positive view into the future. Front Endocrinol (Lausanne). 2018;9:347.CrossRefGoogle Scholar
  11. 11.
    Sugishita Y, Okamoto N, Uekawa A, Yamochi T, Nakajima M, Namba C, et al. Oocyte retrieval after heterotopic transplantation of ovarian tissue cryopreserved by closed vitrification protocol. J Assist Reprod Genet. 2018;35(11):2037–48.CrossRefGoogle Scholar
  12. 12.
    Suzuki N. Research on the effectiveness of cancer and reproductive health care for young cancer patients (fertility preservation), Degree the Ministry of Health, Labor and Welfare of children, Child care support promotion research project. 2018 (in Japanese)Google Scholar
  13. 13.
    Kawamura K, Cheng Y, Suzuki N, Deguchi M, Sato Y, Takae S, et al. Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment. Proc Natl Acad Sci U S A. 2013;110(43):17474–9.CrossRefGoogle Scholar
  14. 14.
    Suzuki N, Yoshioka N, Takae S, Sugishita Y, Tamura M, Hashimoto S, et al. Successful fertility preservation following ovarian tissue vitrification in patients with primary ovarian insufficiency. Hum Reprod. 2015;30(3):608–15.CrossRefGoogle Scholar
  15. 15.
    Amorim CA, Curaba M, Van Langendonckt A, Dolmans MM, Donnes J. Vitrification as an alternative means of cryopreserving ovarian tissue. Reprod BioMed Online. 2011;23(2):160–86.CrossRefGoogle Scholar
  16. 16.
    Abir R, Fisch B, Fisher N, Samara N, Lerer-Serfaty G, Magen R, et al. Attempts to improve human ovarian transplantation outcomes of needle immersed vitrification and slow-freezing by host and graft treatments. J Assist Reprod Genet. 2017;34(5):633–44.CrossRefGoogle Scholar
  17. 17.
    Arav A, Natan Y, Kalo D, Komsky-Elbaz A, Roth Z, Levi-Setti PE, et al. A new, simple, automatic vitrification device; preliminary results with murine and bovine oocytes and embryos. J Assist Reprod Genet. 2018;35(7):1161–8.CrossRefGoogle Scholar
  18. 18.
    Roy TK, Brandi S, Tappe NM, Bradley CK, Vom E, Henderson C, et al. Embryo vitrification using a novel semi-automated closed system yields in vitro outcomes equivalent to the manual Cryotop method. Hum Reprod. 2014;29(11):2431–8.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Obstetrics and GynecologySt. Marianna University School of MedicineKawasaki CityJapan
  2. 2.KRD CorporationYamatoJapan
  3. 3.Its CorporationKawasaki CityJapan
  4. 4.Department of Frontier Medicine Institute of Medical ScienceSt. Marianna University, School of MedicineKawasaki CityJapan

Personalised recommendations