Association of genetic polymorphism of vascular endothelial growth factor in the etiology of recurrent pregnancy loss: a triad study

  • M. Vidyadhari
  • M. Sujatha
  • P. Krupa
  • Pratibha Nallari
  • A. VenkateshwariEmail author



The study estimates the association of VEGF gene polymorphism (-1154 G/A, -2549 I/D, -2578 C/A, and +936 C/T) in recurrent pregnancy loss from South Indian population.


A total of 100 couples with the history of recurrent pregnancy loss and 100 couples with medically terminated pregnancies were considered. Fetal tissues with < 20 weeks of gestation including peripheral blood from case and control couples were collected. VEGF gene polymorphisms were determined by allele-specific polymerase chain reaction. Genotypic distribution and allele frequencies were evaluated by odds ratio with 95% confidence intervals. Haplotype analysis was done to determine the association of specific haplotypes with recurrent pregnancy loss.


The VEGF -1154 G/A polymorphism was significantly prevalent in the aborted fetuses and in their mothers whereas -2549 I/D polymorphism was significantly higher in the aborted fetuses while the + 936 C/T polymorphism showed prevalence in the case mothers revealing their statistically significant association to recurrent pregnancy loss. A1154D2549A2578T936 haplotype showed an increased risk in case fetuses and mothers whereas A1154D2549C2578C936, in case mothers and fathers while haplotype G1154I2549A2578C936 found a protective association in the case fetuses compared to controls.


This is the first report of family-based triad study revealing a significant association of VEGF gene polymorphism in the etiology of recurrent pregnancy loss.


Recurrent pregnancy loss AS-PCR VEGF Polymorphism Fetus 


Funding information

The present work was greatly supported by the Senior Research Fellowship granted by the Indian Council of Medical Research, New Delhi (ICMR–45/1/2014-HUM-BMS).

Compliance with ethical standards

The study was approved by the Ethical Committee of Institute of Genetics, Osmania University (Approval No: 69/IG/IEC/2014 dt 13/08/2014). Demographic details with an informed written consent were obtained before the sample collection from all the subjects.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • M. Vidyadhari
    • 1
  • M. Sujatha
    • 1
  • P. Krupa
    • 2
  • Pratibha Nallari
    • 1
    • 3
  • A. Venkateshwari
    • 1
    Email author
  1. 1.Institute of Genetics and Hospital for Genetic DiseasesOsmania UniversityHyderabadIndia
  2. 2.Government Modern Maternity HospitalHyderabadIndia
  3. 3.Department of GeneticsOsmania UniversityHyderabadIndia

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