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Understanding mechanisms of oocyte development by follicular fluid lipidomics

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The present study aimed to provide a non-invasive approach to studying mechanisms responsible for oocyte development.

Methods

To this end, follicular fluid (FF) from 62 patients undergoing in vitro fertilization (IVF) cycles was split into two groups depending on the pregnancy outcome: pregnant (n = 28) and non-pregnant (n = 34) groups. Data were acquired by the MALDI-TOF mass spectrometry. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to the data set. A ROC curve, to predict success rate, was constructed, and the lipids were attributed.

Results

Six ions were differentially represented in FF of pregnant and non-pregnant patients, with an area under the curve of 0.962. Phosphatidic acid, phosphatidylglycerol, and triacylglycerol were hyper-represented in the pregnant group, while glucosylceramide was hyper-represented in the non-pregnant group. Enriched functions related to these lipids are steroidogenesis, cellular response, signal transduction, cell cycle, and activation of protein kinase C for the pregnant group and apoptosis inhibition for the non-pregnant group.

Conclusion

Human FF fingerprinting can both improve the understanding concerning mechanisms responsible for oocyte development and its effect on embryo implantation potential and assist in the management of IVF cycles.

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Funding

The authors wish to acknowledge the Coordination for the Improvement of Higher Education Personnel (CAPES) for providing funding for this research.

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Authors and Affiliations

  1. Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, Rua Embau, 231, CEP: 04039-060, Sao Paulo, SP, Brazil

    Daniela Antunes Montani, Daniela Paes de Almeida Ferreira Braga, Mariana Camargo, Fernanda Bertuccez Cordeiro, Renato Fraietta & Edson Guimarães Lo Turco

  2. Fertility Medical Group, Av. Brigadeiro Luiz Antônio, 4545, CEP: 04511-010, Sao Paulo, SP, Brazil

    Daniela Paes de Almeida Ferreira Braga & Edson Borges Jr

  3. Laboratorio para Investigaciones Biomédicas, Escuela Superior Politécnica del Litoral, ESPOL, Km. 30.5 Via Perimetral, CEP: 09-01-5863, Guayaquil, Ecuador

    Fernanda Bertuccez Cordeiro

  4. Department of Chemistry, Maringa State University, Avenida Colombo, 5790, CEP: 87020-900, Maringá, PR, Brazil

    Eduardo Jorge Pilau

  5. Institute of Chemistry, University of Campinas, R. Josué de Castro, 126, CEP: 13083-861, Campinas, SP, Brazil

    Fábio Cesar Gozzo

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  1. Daniela Antunes Montani
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Corresponding author

Correspondence to Daniela Paes de Almeida Ferreira Braga.

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The study received approval by the Ethics in Research Committee of Federal University of São Paulo.

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Montani, D.A., Braga, D.P.d.F., Borges, E. et al. Understanding mechanisms of oocyte development by follicular fluid lipidomics. J Assist Reprod Genet 36, 1003–1011 (2019). https://doi.org/10.1007/s10815-019-01428-7

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  • DOI: https://doi.org/10.1007/s10815-019-01428-7

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