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Journal of Assisted Reproduction and Genetics

, Volume 32, Issue 7, pp 1151–1160 | Cite as

Morphokinetic parameters using time-lapse technology and day 5 embryo quality: a prospective cohort study

  • Ashleigh Storr
  • Christos A. Venetis
  • Simon Cooke
  • Daisy Susetio
  • Suha Kilani
  • William Ledger
Assisted Reproduction Technologies

Abstract

Purpose

The aims of this prospective study were to evaluate whether time-lapse parameters can aid in the prediction of day 5 embryo quality and also to assess their discriminatory capacity.

Methods

In this prospective cohort study, we used time-lapse technology to record specific timings of key events for 380 day 5 blastocysts (originating from 108 patients). Generalized estimating equation regression models were used to evaluate the capacity of these markers to identify a top-quality blastocyst. Multivariable regression models were also constructed, aiming to identify the model with the highest capacity to predict a top-quality blastocyst. The discriminatory capacity of single predictors or composite models was assessed with the use of receiver operating characteristic (ROC) analyses.

Results

Eight significant predictive parameters of a top-quality blastocyst were identified: s3, t6, t7, t8, tM, tSB, tB and tEB. A ROC analysis of the identified parameters found s3 (area under the curve—AUC 0.585, 95 % CI 0.534–0.635) to have the best individual discriminatory capacity to predict a top-quality blastocyst prior to embryo compaction. The parameter tEB (AUC 0.727, 95 % CI 0.675–0.775) was the best predictor regardless of embryo stage. A model containing s3, t8 and tEB showed a slightly increased discriminatory capacity for top-quality blastocyst prediction (AUC 0.748, 95 % CI 0.697–0.794).

Conclusions

The identified morphokinetic parameters and their cutoffs, albeit of limited clinical value, add to the increasing knowledge concerning the potential predictive markers of a top-quality blastocyst. Additional evidence is necessary before validated time-lapse parameters can be used for embryo selection in IVF laboratories.

Keywords

Blastocyst Morphokinetics Prediction Time-lapse Quality 

Notes

Acknowledgments

The authors wish to thank all of the IVF Australia nurses involved in patient recruitment as well as the embryology team at IVF Australia—Eastern Suburbs for their continued support.

Compliance with ethical standards

The authors declare that they have no conflict of interest. Consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Ashleigh Storr
    • 1
  • Christos A. Venetis
    • 2
  • Simon Cooke
    • 1
    • 2
  • Daisy Susetio
    • 1
  • Suha Kilani
    • 1
  • William Ledger
    • 1
    • 2
  1. 1.IVF Australia Western SydneyWestmeadAustralia
  2. 2.School of Women’s and Children’s Health, UNSW MedicineUNSWSydneyAustralia

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