Journal of Assisted Reproduction and Genetics

, Volume 32, Issue 2, pp 233–241 | Cite as

Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF

  • Aslıhan Pekel
  • Aymelek Gönenç
  • Nilgün Öztürk Turhan
  • Hasan Kafalı
Assisted Reproduction Technologies

Abstract

Purpose

The Fas-Fas Ligand interaction is one of the essential events for the induction of apoptosis whereas the exact role of their soluble forms in the reproductive system is still not fully understood. Also oxidative stress in the pathogenesis of infertility causing diseases in women and has been suggested as one of the important factors that negatively affect IVF outcome. In this study, our aim was to evaluate serum and follicular fluid levels of soluble Fas soluble Fas Ligand, malondialdehyde, superoxide dismutase and total antioxidant capacity in patients undergoing IVF and compared with controls.

Methods

This study included 109 patients. Patients were classified as unexplained infertility (N = 31), PCOS (N = 19), tubal factor (N = 9) and endometriosis (N = 10) and compared with male factor infertility (N = 40) that was the control group. sFas and sFasL levels were measured by immunoassay method. MDA, SOD and TAC levels were measured by colorimetric method.

Results

Patients with unexplained infertility, PCOS and tubal factor had significantly lower sFas levels compared with their controls (respectively, p < 0.01, p < 0.05, p < 0.05). However, SOD activity in unexplained infertility, PCOS and endometriosisgroupswere significantly higher than control group (p < 0.01).Decreased follicular fluid TAC levels were found in all patient groups compared with controls (respectively, p < 0.01, p < 0.05, p < 0.01, p < 0.01).Patients with tubal factor had significantly higher serum sFasL (p < 0.05), but lower follicular fluid sFasL levels (p < 0.05) compared with unexplained infertility. Tubal factor and endometriosis groups had lowerfollicular fluid TAC levels compared to unexplained infertility and PCOSgroups (p < 0.01).

Conclusion(s)

In this study, serum and follicular fluid sFas levels were decreased and antioxidant activity was impaired in infertility, possibly implying increased apoptosis. Especially in unexplained infertility group changes in this parametres more remarkable.

Keywords

sFas sFasL Oxidative stress Antioxidant IVF 

Notes

Acknowledgments

The work was supported by Gazi University Research Foundation (Project no. 02/2011-36). Our sincere thanks are due to Assoc. Prof. İ.Safa Gürcan, Department of Biometry, Veterinary Faculty, Ankara University for the assistance in data analysis.

Conflict of interest

No conflict of interest.

References

  1. 1.
    von Rango U, Classen-Linke I, Krusche CA, Beier HM. The receptive endometrium is characterized by apoptosis in the glands. Hum Reprod. 1998;13:3177–89.CrossRefGoogle Scholar
  2. 2.
    Malamitsi-Puchner A, Sarandakou A, Baka S, et al. Concentrations of angiogenic factors in follicular fluid and oocyte-cumulus complex culture medium from women undergoing in vitro fertilization. Association with oocyte maturity and fertilization. Fertil Steril. 2001;76:98–101.CrossRefPubMedGoogle Scholar
  3. 3.
    Malamitsi-Puchner A, Sarandakou A, Baka S, Hassiakos D, Kouskouni E, Creatsas G. In vitro fertilization: angiogenic, proliferative and apoptotic factors in follicular fluid. Ann NY Acad Sci. 2003;997:124–8.CrossRefPubMedGoogle Scholar
  4. 4.
    Ueno T, Toi M, Tominaga T. Circulating soluble Fas concentration in breast cancer patients. Clin Cancer Res. 1999;5:3529–33.PubMedGoogle Scholar
  5. 5.
    Srivastava M, Lippes J, Pichorova R, De los Santos, Anderson DJ. Soluble Fas (sFas) and soluble Fas ligand (sFasL) in human reproductive tract fluids. In: Proceedings of the 45th Annual Meeting of the Society for the Gynecologic Investigation, Chicago; 1998. p.T203[abstract].Google Scholar
  6. 6.
    Sarandakou A, Malamitsi-Puchner A, Baka S, Rizos D, Hassiakos D, Creatsas G. Apoptosis and proliferation factors in serum and follicular fluid from women undergoing in vitro fertilization. Fertil Steril. 2003;79:634–6.CrossRefPubMedGoogle Scholar
  7. 7.
    Murphy AA, Santanam N, Parthasarathy S. Endometriosis: a disease of oxidative stress? Semin Reprod Endocrinol. 1998;16(4):263–73.CrossRefPubMedGoogle Scholar
  8. 8.
    Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005;3:28.CrossRefPubMedCentralPubMedGoogle Scholar
  9. 9.
    Agarwal A, Said TM, Bedaiwy MA, Banerjee J, Alvarez JG. Oxidative stress in an assisted reproductive techniques setting. Fertil Steril. 2006;86(3):503–12.CrossRefPubMedGoogle Scholar
  10. 10.
    Marsillach J, Checa MA, Pedro-Botet J, Carreras R, Joven J, Camps J. Paraoxonase-1 in female infertility: a possible role against oxidative stress-induced inflammation. Fertil Steril. 2010;94(3):1132–4.CrossRefPubMedGoogle Scholar
  11. 11.
    Attaran M, Pasqualotto E, Falcone T, et al. The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization. Int J Fertil Womens Med. 2000;45(5):314–20.PubMedGoogle Scholar
  12. 12.
    Oyawoye O, Abdel Gadir A, Garner A, Constantinovici N, Perrett C, Hardiman P. Antioxidants and reactive oxygen species in follicular fluid of women undergoing IVF: relationship to outcome. Hum Reprod. 2003;18(11):2270–4.CrossRefPubMedGoogle Scholar
  13. 13.
    McCord JM, Fridovich I. The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen. J Biol Chem. 1969;244(22):6056–63.PubMedGoogle Scholar
  14. 14.
    Agarwal A, Said TM. Oxidative stres, DNA damage and apoptosis in male infertility: a clinical approach. BJU Int. 2005;95(4):503–37.CrossRefPubMedGoogle Scholar
  15. 15.
    Pasqualotto EB, Agarwal A, Sharma RK, et al. Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures. Fertil Steril. 2004;81(4):973–6.CrossRefPubMedGoogle Scholar
  16. 16.
    Wiener-Megnazi Z, Vardi L, Lissak A, et al. Oxidative stress indices in follicular fluid as measured by the thermochemiluminescence assay correlate with outcome parameters in in vitro fertilization. Fertil Steril. 2004;82 Suppl 3:1171–6.CrossRefPubMedGoogle Scholar
  17. 17.
    Das S, Chattopadhyay R, Ghosh S, et al. Reactive oxygen species level in follicular fluid-embryo quality marker in IVF? Hum Reprod. 2006;21(9):2403–7.CrossRefPubMedGoogle Scholar
  18. 18.
    Mor G, Straszewski S, Kamsteeg. The Fas/Fas ligand system in reproduction: survival and apoptosis. The Sci World J. 2002;2:1828–42.CrossRefGoogle Scholar
  19. 19.
    Abdelmeged AN, Yasser IA, Elmoghazi DE, Eissa MK. Evaluation of sFas in serum and follicular fluid during ovarian stimulation for assisted reproduction. Middle East Fertil Soc J. 2011;16(1):50–5.CrossRefGoogle Scholar
  20. 20.
    Onalan G, Selam B, Baran Y, et al. Serum and follicular fluid levels of soluble Fas, soluble Fas ligand and apoptosis of luteinized granulosa cells in PCOS patients undergoing IVF. Hum Reprod. 2005;20(9):2391–5.CrossRefPubMedGoogle Scholar
  21. 21.
    Onalan G, Selam B, Onalan R, Geyhan T, Cincik M, Pabuccu R. Serum and follicular fluid levels of soluble Fas and soluble Fas ligand in IVF cycles. Eur J Obstet Gynecol Reprod Biol. 2006;125:85–91.CrossRefPubMedGoogle Scholar
  22. 22.
    Garcia-Velasco JA, Mulayim N, Kayisli UA, Arici A. Elevated soluble Fas ligand levels may suggest a role for apoptosis in women with endometriosis. Fertil Steril. 2002;78(4):855–9.CrossRefPubMedGoogle Scholar
  23. 23.
    Hockenbery DM, Oltvai ZN, Yin XM, Milliman CL, Korsmeyer SJ. Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell. 1993;75:241–51.CrossRefPubMedGoogle Scholar
  24. 24.
    Bojes HK, Datta K, Xu J, et al. Bcl-xL overexpression attenuates glutathione depletion in FL5.12 cells following interleukin-3 withdrawal. Biochem J. 1997;325:115–9.Google Scholar
  25. 25.
    Dimmeler S, Haendeler J, Sause A, Zeiher AM. Nitric oxide inhibits APO-1/Fas-mediated cell death. Cell Growth Differ. 1998;9:415–22.PubMedGoogle Scholar
  26. 26.
    Tamarit J, Cabiscol E, Ros J. Identification of the major oxidatively damaged proteins in Escherichia coli cells exposed to oxidative stress. J Biol Chem. 1998;273:3027–32.CrossRefPubMedGoogle Scholar
  27. 27.
    Hunt CR, Sim JE, Sullivan SJ, et al. Genomic instability and catalase gene amplifcation induced by chronic exposure tooxidative stress. Cancer Res. 1998;58:3986–92.PubMedGoogle Scholar
  28. 28.
    Pasqualotto EB, Lara LV, Salvador M, Sobreiro BP, Borges E, Pasqualotto FF. The role of enzymatic antioxidants detected in the follicular fluid and semen of infertile couples undergoing assisted reproduction. Hum Fertil (Camb). 2009;12(3):166–71.CrossRefGoogle Scholar
  29. 29.
    Chattopadhayay R, Ganesh A, Samanta J, Jana SK, Chakravarty BN, Chaudhury K. Effect of follicular fluid oxidative stres on meiotic spindle formation in infertile women with polycyctic ovarian syndrome. Gynecol Obstet Invest. 2010;69:197–202.CrossRefPubMedGoogle Scholar
  30. 30.
    Prieto L, Quesada JF, Cambero O, et al. Analysis of follicular fluid and serum markers of oxidative stress in women with infertility related to endometriosis. Fertil Steril. 2012;98(1):126–30.CrossRefPubMedGoogle Scholar
  31. 31.
    Agarwal A, Gupta S, Sekhon L, Shah R. Redox considerations in female reproductive function and assisted reproduction: from molecular mechanisms to health implications. Antioxid Redox Signal. 2008;10(8):1375–403.CrossRefPubMedGoogle Scholar
  32. 32.
    Chandra A, Surti N, Kesavan S, Agarwal A. Significance of oxidative stress in human reproduction. Arch Med Sci. 2009;5(1A):S28–42.Google Scholar
  33. 33.
    Sabatini L, Wilson C, Lower A, Al-Shawaf T, Grudzinskas JG. Superoxide dismutase activity in human follicular fluid after controlled ovarian hyperstimulation in women undergoing in vitro fertilization. Fertil Steril. 1999;72(6):1027–34.CrossRefPubMedGoogle Scholar
  34. 34.
    Younis A, Clower C, Nelsen D, et al. The relationship between pregnancy and oxidative stress markers on patients undergoing ovarian stimulations. Assist Reprod Genet. 2012;29(10):1083–9.CrossRefGoogle Scholar
  35. 35.
    Li J, Foote RH, Simkin M. Development of rabbit zygotes cultured in protein-free medium with catalase, taurine, or superoxide dismutase. Biol Reprod. 1993;49:33–7.CrossRefPubMedGoogle Scholar
  36. 36.
    Gönenç A, Hacışevki A, Tavil Y, Çengel A, Torun M. Oxidative stress in patients with essential hypertension: a comparison of dippers and non-dippers. Eur J Int Med. 2013;24:139–44.CrossRefGoogle Scholar
  37. 37.
    Gönenç A, Atak Y, Orman MN, Şimşek B. Lipid peroxidation and antioxidant systems in hemodialyzed patients. Dial Transplant. 2002;31:88–96.Google Scholar
  38. 38.
    Gönenç A, Hacışevki A, Griffiths HR, Torun M, Bakkaloğlu B, Şimşek B. Free radical reaction products and antioxidant capacity in beating heart coronary artery surgery compared to conventional bypass. Biochem Moscow. 2011;76:677–85.CrossRefGoogle Scholar
  39. 39.
    Bedaiwy M, Agarwal A, Said TM, et al. Role of total antioxidant capacity in the differential growth of human embryos in vitro. Fertil Steril. 2006;86(2):304–9.CrossRefPubMedGoogle Scholar
  40. 40.
    Appasamy M, Jauniaux E, Serhal P, Al-Qahtani A, Groome NP, Muttukrishna S. Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation. Fertil Steril. 2008;89(4):912–21.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Aslıhan Pekel
    • 1
  • Aymelek Gönenç
    • 2
  • Nilgün Öztürk Turhan
    • 3
  • Hasan Kafalı
    • 4
  1. 1.IVF Clinic, Department of Obstetrics and Gynecology, Faculty of MedicineTurgut Özal UniversityAnkaraTurkey
  2. 2.Department of Biochemistry, Faculty of PharmacyGazi UniversityAnkaraTurkey
  3. 3.Department of Obstetrics and Gynecology, Faculty of MedicineMuğla Sıtkı Koçman UniversityMuğlaTurkey
  4. 4.Department of Obstetrics and Gynecology, Faculty of MedicineTurgut Özal UniversityAnkaraTurkey

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