Improvement of mouse embryo quality by myo-inositol supplementation of IVF media
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Myo-inositol (myoIns) has a positive role in mammalian development and human reproduction. Since experiments on farming species suggest a similar role in preimplantation development, we evaluated the hypothesis that the inclusion of myoIns in human embryo culture media would produce an increase in embryo quality in IVF cycles, using the mouse embryo assay.
To determine the effect of myoIns on completion of preimplantation development in vitro, one-cell embryos of the inbred C57BL/6N mouse strain were produced by ICSI, cultured in human fertilization media in the presence of myoIns (myoIns+) or in its absence (myoIns–) and evaluated morphologically. Daily progression through cleavage stages, blastocyst production and expansion and blastomere number at 96 hours post fertilization were assessed.
Compared to myoIns– embryos, myoIns+ embryos displayed a faster cleavage rate and by the end of preimplantation development, the majority of myoIns+ blastocysts was expanded and formed by a higher number of blastomeres.
The presence of myoIns resulted in both an increase in proliferation activity and developmental rate of in vitro cultured early mouse embryos, representing a substantial improvement of culture conditions. These data may identify myoIns as an important supplement for human embryo preimplantation culture.
KeywordsIn vitro fertilization Preimplantation embryo culture Myo-inositol Embryo quality Mouse
This work was financially supported by “Ateneo” grants to AB.
We thank Lo.Li. Pharma for kindly providing Andrositol®LAB. We are also grateful to Dr. Robert P. Erickson for critically reading of the manuscript.
- 10.D’Anna R, Scilipoti A, Giordano D, Caruso C, Cannata ML, Interdonato ML, et al. Myo-Inositol supplementation and onset of gestational diabetes mellitus in pregnant women with a family history of type 2 diabetes: a prospective, randomized, placebo-controlled study. Diabetes Care. 2013;36(4):854–7.PubMedPubMedCentralCrossRefGoogle Scholar
- 21.Howlett SK, Bolton VN. Sequence and regulation of morphological and molecular events during the first cell cycle of mouse embryogenesis. J Embryol Exp Morpholog. 1985;87:175–206.Google Scholar
- 24.Kelly SJ, Mulnard JG, Graham CF. Cell division and cell allocation in early mouse development. J Embryol Exp Morpholog. 1978;48:37–51.Google Scholar
- 29.Kwon HM, Yamauchi A, Uchida S, Preston AS, Garcia-Perez A, Burg MB, et al. Cloning of the cDNA for a Na+/myo-inositol cotransporter, a hypertonicity stress protein. JBC. 1992;267:6297–301.Google Scholar
- 32.Lim JJ, Eum JH, Lee JE, Kim ES, Chung HM, Yoon TK, et al. Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes. J Assist Reprod Genet. 2010;27(11):619–27.PubMedCentralPubMedCrossRefGoogle Scholar