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Journal of Assisted Reproduction and Genetics

, Volume 31, Issue 4, pp 435–442 | Cite as

Altered cleavage patterns in human tripronuclear embryos and their association to fertilization method: A time-lapse study

  • Mette Warming JoergensenEmail author
  • Inge Agerholm
  • Johnny Hindkjaer
  • Lars Bolund
  • Lone Sunde
  • Hans Jakob Ingerslev
  • Kirstine Kirkegaard
Embryo Biology

Abstract

Purpose

To analyze the cleavage patterns in dipronuclear (2PN) and tripronuclear (3PN) embryos in relation to fertilization method.

Method

Time-lapse analysis.

Results

Compared to 2PN, more 3PN IVF embryos displayed early cleavage into 3 cells (p < 0.001), displayed longer duration of the 3-cell stage (p < 0.001), and arrested development from the compaction stage and onwards (p < 0.001). For the IVF embryos, the 2nd and 3rd cleavage cycles were completed within the expected time frame. However, timing of the cell divisions within the cleavage cycles differed between the two groups. In contrast, the completion of the 1st, 2nd, and 3rd cleavage cycle was delayed, but with a similar division pattern for 3PN ICSI compared with the 2PN ICSI embryos. 3PN, more often than 2PN ICSI embryos, displayed early cleavage into 3 cells (p = 0.03) and arrested development from the compaction stage and onwards (p = 0.001). More 3PN IVF than ICSI embryos displayed early cleavage into 3 cells (p < 0.001).

Conclusions

This study reports differences in cleavage patterns between 2PN and 3PN embryos and for the first time demonstrates differences in the cleavage pattern between 3PN IVF and ICSI embryos.

Keywords

Cell division Embryo Humans Triploidy Time-lapse 

Notes

Acknowledgments

The authors wish to thank the clinical, paramedical, and laboratory team of the Fertility Clinic, Aarhus University Hospital. The study was supported by Aarhus University, University of Southern Denmark, Aase and Einar Danielsen Foundation, Lipperts Foundation, the Augustinus Foundation and the Toyota Foundation. Research at the Fertility Clinic is funded by an unrestricted grant from Ferring and MSD.

Conflict of interest

M.W.J., J.H., L.B., L.S., H.J.I, and K.K, declare no conflicts of interest. I.A. works part-time as a scientific consultant for Unisense FertiliTech and holds stocks in the company.

Supplementary material

ESM 1

(MPEG 13782 kb)

10815_2014_178_MOESM2_ESM.pdf (32 kb)
ESM 2 (PDF 31 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Mette Warming Joergensen
    • 1
    • 2
    Email author
  • Inge Agerholm
    • 3
  • Johnny Hindkjaer
    • 4
  • Lars Bolund
    • 5
  • Lone Sunde
    • 5
    • 6
  • Hans Jakob Ingerslev
    • 4
  • Kirstine Kirkegaard
    • 4
    • 7
  1. 1.Department of Clinical GeneticsVejle HospitalVejleDenmark
  2. 2.Institute of Regional Health ResearchUniversity of Southern DenmarkOdenseDenmark
  3. 3.The Fertility ClinicHorsens HospitalHorsensDenmark
  4. 4.The Fertility Clinic and Centre for Preimplantation Genetic DiagnosisAarhus University HospitalSkejbyDenmark
  5. 5.Department of BiomedicineAarhus UniversityAarhusDenmark
  6. 6.Department of Clinical GeneticsAarhus University HospitalSkejbyDenmark
  7. 7.Department of Clinical BiochemistryAarhus University HospitalSkejbyDenmark

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