Journal of Assisted Reproduction and Genetics

, Volume 30, Issue 4, pp 525–530 | Cite as

High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations

  • Mohamed Hassen Chelli
  • Fatma Ferfouri
  • Florence Boitrelle
  • Martine Albert
  • Denise Molina-Gomes
  • Jacqueline Selva
  • François Vialard
Genetics

Abstract

Problem

This study sought to evaluate the value of motile sperm organelle morphology examination (MSOME) for selecting euploid spermatozoa in six patients who were heterozygous for a reciprocal translocation.

Method of study

We used sperm fluorescence in situ hybridization (FISH) to screen for aneuploidy of the chromosomes involved in the translocations and a putative interchromosomal effect (ICE) for chromosomes 18, X and Y. This procedure was performed on (i) whole sperm (i.e. no selection) and on normal spermatozoa selected (ii) at a magnification typically used for intracytoplasmic sperm injection (ICSI), referred to as “ICSI-like”, and (iii) with MSOME.

Results

The balanced translocation rates did not differ significantly (p = 0.81) when comparing whole sperm (57.2 %) with spermatozoa after ICSI-like selection (56.3 %) or after MSOME (53.7 %). Similarly, the aneuploidy rates for ICEs did not differ significantly (p = 0.14) when comparing whole sperm (1.9 %), ICSI-selected spermatozoa (3.4 %) and MSOME-selected spermatozoa (1.0 %).

Conclusion

For patients who are heterozygous for reciprocal translocations, MSOME does not improve the selection of euploid spermatozoa.

Keywords

Sperm FISH IMSI MSOME Reciprocal translocation Interchromosomal effect (ICE) 

Notes

Acknowledgment

This work was funded by the French Biomedicine Agency.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Mohamed Hassen Chelli
    • 1
    • 3
  • Fatma Ferfouri
    • 1
    • 2
  • Florence Boitrelle
    • 1
    • 2
  • Martine Albert
    • 1
    • 2
  • Denise Molina-Gomes
    • 1
  • Jacqueline Selva
    • 1
    • 2
  • François Vialard
    • 1
    • 2
  1. 1.Department of Reproductive Biology, CytogeneticsGynaecology and Obstetrics, CHIPS, Centre Hospitalier Poissy Saint-GermainPoissyFrance
  2. 2.EA 2493, University of Versailles Saint-Quentin en Yvelines (UVSQ)VersaillesFrance
  3. 3.Department of Reproductive BiologyNouvelle Clinique du ParcTunisTunisia

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