High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations
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This study sought to evaluate the value of motile sperm organelle morphology examination (MSOME) for selecting euploid spermatozoa in six patients who were heterozygous for a reciprocal translocation.
Method of study
We used sperm fluorescence in situ hybridization (FISH) to screen for aneuploidy of the chromosomes involved in the translocations and a putative interchromosomal effect (ICE) for chromosomes 18, X and Y. This procedure was performed on (i) whole sperm (i.e. no selection) and on normal spermatozoa selected (ii) at a magnification typically used for intracytoplasmic sperm injection (ICSI), referred to as “ICSI-like”, and (iii) with MSOME.
The balanced translocation rates did not differ significantly (p = 0.81) when comparing whole sperm (57.2 %) with spermatozoa after ICSI-like selection (56.3 %) or after MSOME (53.7 %). Similarly, the aneuploidy rates for ICEs did not differ significantly (p = 0.14) when comparing whole sperm (1.9 %), ICSI-selected spermatozoa (3.4 %) and MSOME-selected spermatozoa (1.0 %).
For patients who are heterozygous for reciprocal translocations, MSOME does not improve the selection of euploid spermatozoa.
KeywordsSperm FISH IMSI MSOME Reciprocal translocation Interchromosomal effect (ICE)
This work was funded by the French Biomedicine Agency.
- 10.Cassuto NG, Le Foll N, Chantot-Bastaraud S, Balet R, Bouret D, Rouen A, et al. Sperm fluorescence in situ hybridization study in nine men carrying a Robertsonian or a reciprocal translocation: relationship between segregation modes and high-magnification sperm morphology examination. Fertil Steril. 2011;96(4):826–32.PubMedCrossRefGoogle Scholar
- 11.Chelli MH, Albert M, Ray PF, Guthauser B, Izard V, Hammoud I, et al. Can intracytoplasmic morphologically selected sperm injection be used to select normal-sized sperm heads in infertile patients with macrocephalic sperm head syndrome? Fertil Steril. 2009;93(4):1347 e1341–1345.Google Scholar
- 17.Franco JG, Mauri AL, Petersen CG, Massaro FC, Silva LF, Felipe V, Cavagna M, Pontes A, Baruffi RL, Oliveira JB, Vagnini LD (2011) Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int J Androl.Google Scholar
- 24.Palermo G, Hu J, Rienzi L, Maggiulli R, Takeuchi T, Yoshida A, et al. Thoughts on IMSI. New York: Springer; 2011. p. 277–89.Google Scholar
- 26.Pellestor F, Puechberty J, Weise A, Lefort G, Anahory T, Liehr T, Sarda P (2011) Meiotic segregation of complex reciprocal translocations: direct analysis of the spermatozoa of a t(5;13;14) carrier. Fertil Steril.Google Scholar
- 29.Rousseaux S, Chevret E, Monteil M, Cozzi J, Pelletier R, Devillard F, et al. Meiotic segregation in males heterozygote for reciprocal translocations: analysis of sperm nuclei by two and three colour fluorescence in situ hybridization. Cytogenet Cell Genet. 1995;71(3):240–6.PubMedCrossRefGoogle Scholar
- 33.Wilding M, Coppola G, di Matteo L, Palagiano A, Fusco E, Dale B. Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J Assist Reprod Genet. 2011;28(3):253–62.PubMedCrossRefGoogle Scholar
- 34.World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th ed. Cambridge: Cambridge University Press; 2009.Google Scholar