Journal of Assisted Reproduction and Genetics

, Volume 30, Issue 3, pp 305–314 | Cite as

A review of 15 years of ovarian tissue bank activities

  • Marie-Madeleine Dolmans
  • Pascale Jadoul
  • Sébastien Gilliaux
  • Christiani A. Amorim
  • Valérie Luyckx
  • Jean Squifflet
  • Jacques Donnez
  • Anne Van Langendonckt
Fertility Preservation



To review 15 years of activities in ovarian tissue cryobanking from medical database files, including patient indications, histological evaluation and clinical characteristics.


Retrospective longitudinal analysis of data from an ovarian tissue bank in an academic hospital. Five hundred and eighty-two patients had their ovarian tissue cryobanked between April 1997 and January 2012. Analysis of cryobanking database: precryopreservation patient characteristics, indications and safety issues, laboratory files and postcryopreservation clinical data.


Of the 582 patients who had their ovarian tissue cryopreserved, 106 patients donated for research purposes and 476 patients for fertility preservation and long-term cryopreservation. Clinical data analysis of the 476 patients revealed a mean age at the time of cryopreservation of 23 ± 8.5 years (range: 9 months – 39 years), with 96.2 % of subjects aged ≤35 years (n = 458). Among 391 cases of malignant disease, hematological malignancies (39.9 %, n = 156) and breast cancer (21.7 %, n = 85) were the two main indications. At histology, malignant cells were found in ovarian tissue from leukemia patients (n = 3) and non-Hodgkin’s lymphoma patients (n = 2). Eleven patients underwent autotransplantation, resulting in 5 live births and 1 ongoing pregnancy.


This is the largest and most comprehensive study to describe and analyze indications and clinical patient characteristics before and after ovarian tissue cryopreservation. The procedure is safe, easy and promising. The database concept is a useful tool in patient selection for autotransplantation.


Chemotherapy Cancer Premature ovarian failure Ovarian tissue cryopreservation Database Malignant cells Cryobank 



The present study was supported by grants from the Fonds National de la Recherche Scientifique de Belgique (grant Télévie N0 7.4507.10, grant 3.4.590.08 awarded to Marie-Madeleine Dolmans), the Fonds Spéciaux de Recherche, the Fondation Saint-Luc, the Foundation Against Cancer, and donations from Mr Pietro Ferrero, Baron Frère and Viscount Philippe de Spoelberch. We thank Mélanie Gorez and Cristina Marinescu for their help with data completion of the database.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Marie-Madeleine Dolmans
    • 1
    • 2
  • Pascale Jadoul
    • 1
    • 2
  • Sébastien Gilliaux
    • 1
  • Christiani A. Amorim
    • 1
  • Valérie Luyckx
    • 1
    • 2
  • Jean Squifflet
    • 1
    • 2
  • Jacques Donnez
    • 1
    • 2
  • Anne Van Langendonckt
    • 1
  1. 1.Department of Gynecology, Institut de Recherche Expérimentale et Clinique (IREC)Université Catholique de LouvainBrusselsBelgium
  2. 2.Department of GynecologyCliniques Universitaires Saint LucBrusselsBelgium

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