Comparison of clinical outcomes between single and double vitrified-warmed blastocyst embryo transfer according to the day of vitrification

  • Sang Min Kang
  • Sang Won Lee
  • San Hyun Yoon
  • Joo Cheol Kim
  • Jin Ho Lim
  • Seong Goo Lee



To compare the efficacy of single vitrified-warmed blastocyst embryo transfer (SVBT) versus double vitrified-warmed blastocyst embryo transfer (DVBT) according to the day of vitrification.


This retrospective study included a total of 1,051 cycles in women less than 37 years of age with their autologous SVBT cryopreserved on day 5 (5d-SVBT, n = 737) or day 6 (6d-SVBT, n = 154) and DVBT on day 5 (5d-DVBT, n = 129) or day 6 (6d-DVBT, n = 31) from January 2009 to December 2011.


The clinical pregnancy rate (41.8 % vs. 48.1 %, p = 0.184) and ongoing pregnancy rate (36.6 % vs. 45.0 %, p = 0.072) were not significantly different between the 5d-SVBT group and the 5d-DVBT group. However, the clinical pregnancy (29.9 % vs. 58.1 %, p = 0.003) and ongoing pregnancy rates (23.4 % vs. 51.6 %, p = 0.001) were significantly lower in the 6d-SVBT group compared with those in the 6d-DVBT group. The implantation rate (42.2 % vs. 34.5 %, p = 0.03) of the 5d-SVBT group was significantly higher than that of the 5d-DVBT group, while the implantation rate (29.9 % vs. 37.1 %, p = 0.303) of the 6d-SVBT group was not statistically different compared with that in the 6d-DVBT group. The multiple pregnancy rates (1.0 % in the 5d-SVBT group vs. 38.7 % in the 5d-DVBT group, p < 0.001 and 0 % in the 6d-SVBT group vs. 22.2 % in the 6d-DVBT group, p = 0.001) were statistically significantly lower in the SVBT group compared with those in the DVBT group regardless of the day of vitrification.


This study showed that the 5d-SVBT resulted in comparable clinical outcomes compared to the 5d-DVBT while the 6d-SVBT yielded significantly lower clinical outcomes compared to the 6d-DVBT.


Single embryo transfer Single vitrified-warmed blastocyst embryo transfer Double vitrified-warmed blastocyst embryo transfer Vitrification Multiple pregnancies 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Sang Min Kang
    • 1
  • Sang Won Lee
    • 1
  • San Hyun Yoon
    • 2
  • Joo Cheol Kim
    • 1
  • Jin Ho Lim
    • 2
  • Seong Goo Lee
    • 1
  1. 1.Maria Fertility ClinicDaeguRepublic of Korea
  2. 2.Seoul Maria Fertility HospitalSeoulRepublic of Korea

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