Journal of Assisted Reproduction and Genetics

, Volume 30, Issue 9, pp 1231–1238 | Cite as

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

  • Bayasula
  • Akira IwaseEmail author
  • Hiroharu Kobayashi
  • Maki Goto
  • Tatsuo Nakahara
  • Tomoko Nakamura
  • Mika Kondo
  • Yoshinari Nagatomo
  • Tomomi Kotani
  • Fumitaka Kikkawa
Technological Innovations



Human follicular fluid constitutes the microenvironment of follicles and includes various biological active proteins that can affect follicle growth and oocyte fertilization. Conducting proteomic evaluations of human follicular fluid may be helpful for identifying potential biomarkers possibly possessing a predictive value for oocyte quality and the success of in vitro fertilization.


We performed proteomic profiling of human follicular fluids containing oocytes that were fertilized and resulted in pregnancy and follicular fluids containing oocytes that were not fertilized in the same patients undergoing intracytoplasmic sperm injection using the LTQ Orbitrap coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses.


We identified a total of 503 proteins in human follicular fluids containing fertilized and non-fertilized oocytes obtained from 12 patients. We also found that 53 proteins exhibited significantly different spectral counts between the two groups, including heparan sulfate proteoglycan perlecan, which showed significant upregulation in the follicular fluids containing fertilized oocytes in comparison with that observed in the follicular fluids containing non-fertilized oocytes.


Our results suggest a possibility that proteins identified by LC/MS/MS in follicular fluid might not only be involved in folliculogenesis, but also function as biomarkers possessing predictive potential for oocyte maturation and the success of IVF when their expression levels are significantly different between fertilized and non-fertilized oocytes, although no distinctive biomarkers were identified in the current study.


Follicular fluid Heparan sulfate proteoglycan perlecan IVF LC/MS/MS Proteomic analysis 



This study was supported by Yamaguchi Endocrine Research Foundation.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10815_2013_4_MOESM1_ESM.doc (418 kb)
Supplemental Table 1 (DOC 417 kb)


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Bayasula
    • 1
  • Akira Iwase
    • 1
    • 2
    Email author
  • Hiroharu Kobayashi
    • 1
  • Maki Goto
    • 1
    • 2
  • Tatsuo Nakahara
    • 1
  • Tomoko Nakamura
    • 1
  • Mika Kondo
    • 1
  • Yoshinari Nagatomo
    • 1
  • Tomomi Kotani
    • 1
    • 2
  • Fumitaka Kikkawa
    • 1
  1. 1.Department of Obstetrics and GynecologyNagoya University Graduate School of MedicineNagoyaJapan
  2. 2.Department of Maternal and Perinatal MedicineNagoya University HospitalNagoyaJapan

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