Spontaneous in vitro maturation of oocytes prior to ovarian tissue cryopreservation in natural cycles of oncologic patients
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To evaluate the recovery rate and spontaneous in vitro maturation (IVM) of immature oocytes enclosed within or released from follicles during the processing of ovarian tissue prior to its cryopreservation.
Thirty-three oncologic patients who had not previously undergone chemo or radiotherapy underwent ovarian tissue cryopreservation (OTC) during natural menstrual cycles. Immature oocytes, enclosed within follicles or released during ovarian cortex processing, were collected and matured spontaneously in vitro for 48 h. Nuclear maturation was assessed every 24 h and the ability of the IVM oocytes to display a normal activation response following parthenogenetic activation was evaluated. The following outcome measures were also evaluated: disease, age, FSH, LH, E2, P4 and AMH serum levels, menstrual cycle day, recovery and spontaneous IVM and parthenogenetic activation rates.
Oocytes recovered per patient were 3.3 ± 0.7 (1.8–4.7 oocytes, 95CI), regardless of the menstrual phase. The mean number of IVM oocytes per patient was 1.3 ± 0.2 oocytes (95CI: 0.8–1.8), regardless of menstrual phase (p = 0.86) and oocyte origin (p = 0.61). Forty-one percent of oocytes extruded the second polar body and formed one pronucleus after parthenogenetic activation.
Twenty-one of the 33 women (63.6 %) requesting OTC produced at least one mature oocyte.
KeywordsOvarian tissue cryopreservation Immature oocyte Spontaneous in vitro maturation Human Oncologic patient Natural cycles
The authors would like to thank Mr. Normanly for editing this manuscript. Authors would also like to thank the Hospital Universitario Dr. Peset de Valencia for providing the biological samples and analyzing blood samples. This work was funded by IMPIVA (Instituto de la Mediana y Pequeña Empresa Valenciana; IMIDTF/2009/133; IMIDTF/2010/82, IMIDTF/2011/203 and Generalitat Valenciana) and Instituto Universitario IVI Valencia. The authors declare that they have no conflict of interest.
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